Temperature-dependent Index of Mitotic Interval (τ0) for Chromosome Manipulation in Korean Bullhead, Pseudobagrus fulvidraco
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참고문헌
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초록
Korean bullhead (Pseudobagrus fulvidraco) was collected from the Kum River areas of Kangkyung-eup,Nonsan city, Chungcheongnam-do, Korea, from April to June, 2012 and was fertilized in order to observe egg development and temperature-related cleavage rates and mitotic intervals (τ0). The fertilized eggs were separative, demersal and light yellowish with 1.5±0.06 mm in diameter, and did not contain oil globules. The first cleavage stages were 90 min, 80 min,60 min and 50 min at 21℃, 24℃, 27℃ and 30℃, respectively. At higher temperatures, eggs developed faster and underwent further identical development. For Korean bullhead, τ0 were 33.4±2.08 min at 21℃, 31.5±3.06 min at 24℃, 28.1±2.11 min at 27℃ and 26.4±3.35 min at 30℃. There were strong negative correlations between the τ0 and water temperatures at all points studied (Y=－1.13X+58.15, R^2=0.98, n=30, where Y is τ0 and X is temperature). The results obtained in this work will be helpful for chromosome manipulation by use of cleavage frequency data and τ0 data in Korean bullhead.
Abstract
Korean bullhead (Pseudobagrus fulvidraco) was collected from the Kum River areas of Kangkyung-eup,Nonsan city, Chungcheongnam-do, Korea, from April to June, 2012 and was fertilized in order to observe egg development and temperature-related cleavage rates and mitotic intervals (τ0). The fertilized eggs were separative, demersal and light yellowish with 1.5±0.06 mm in diameter, and did not contain oil globules. The first cleavage stages were 90 min, 80 min,60 min and 50 min at 21℃, 24℃, 27℃ and 30℃, respectively. At higher temperatures, eggs developed faster and underwent further identical development. For Korean bullhead, τ0 were 33.4±2.08 min at 21℃, 31.5±3.06 min at 24℃, 28.1±2.11 min at 27℃ and 26.4±3.35 min at 30℃. There were strong negative correlations between the τ0 and water temperatures at all points studied (Y=－1.13X+58.15, R^2=0.98, n=30, where Y is τ0 and X is temperature). The results obtained in this work will be helpful for chromosome manipulation by use of cleavage frequency data and τ0 data in Korean bullhead.
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