Temperature Dependent of Mitotic Interval for Grass Puffer, Takifugu niphobles
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초록
The objective of this study was to determine the mitotic intervals (τ0) of two consecutive cell divisions and synchronous embryonic cleavage in grass puffer, Takifugu niphobles at different water temperatures (18, 20, 22, and 24℃). The color of the fertilized egg was light yellowish. The egg type was demersal and unadhesive. Egg weight was 0.09±0.002 mg. The sizes of unfertilized eggs were smaller than fertilized eggs in major axis and minor axis at 20℃ (p<0.05). The size of the fertilized egg of 18℃ water temperature group at the blastodisc stage was the smallest (p<0.05), but no significant differ-ences were observed in the other water temperatures group except 18℃ water temperature group (p>0.05). The first cleavage stages at 18, 20, 22, and 24℃ were at 75, 90, 105, and 120 mins, respectively. As water temperature was increased, embryonic development and formation time of the first cleavage furrow were accelerated. There were negative correlation between τ0 and water temperature for grass puffer (Y=–1.225X+70.05, R2=0.988, n=10, where Y was τ0 and X was temperature). This study confirmed that successful hatching of grass puffer was related to water temperature. Chromosome manipulation will be help-ful for this species using cleavage frequency and τ0.
Abstract
The objective of this study was to determine the mitotic intervals (τ0) of two consecutive cell divisions and synchronous embryonic cleavage in grass puffer, Takifugu niphobles at different water temperatures (18, 20, 22, and 24℃). The color of the fertilized egg was light yellowish. The egg type was demersal and unadhesive. Egg weight was 0.09±0.002 mg. The sizes of unfertilized eggs were smaller than fertilized eggs in major axis and minor axis at 20℃ (p<0.05). The size of the fertilized egg of 18℃ water temperature group at the blastodisc stage was the smallest (p<0.05), but no significant differ-ences were observed in the other water temperatures group except 18℃ water temperature group (p>0.05). The first cleavage stages at 18, 20, 22, and 24℃ were at 75, 90, 105, and 120 mins, respectively. As water temperature was increased, embryonic development and formation time of the first cleavage furrow were accelerated. There were negative correlation between τ0 and water temperature for grass puffer (Y=–1.225X+70.05, R2=0.988, n=10, where Y was τ0 and X was temperature). This study confirmed that successful hatching of grass puffer was related to water temperature. Chromosome manipulation will be help-ful for this species using cleavage frequency and τ0.
참고문헌 (26)
- Ahn CM, "Effects of photoperiod and water temperature on the reproductive cycle of the spring-spawning bitterling, Rhodeus uyekii (Pisces: Cyprinidae)", Korean J Ichthyol, 7, pp.33~42 (1995)
- Dettlaff TA, "The rate of development in poikilothermic animals calculated in astronomical and relative time units", J Therm Biol, 11, pp.1~7 (1986)[DOI]
- 길현우, "Anesthetic and Physiological Effects of Clove oil and Lidocaine-HCl on the Grass Puffer, Takifugu niphobles", Ocean and Polar Research, 39(1), pp.1~11 (2017), 한국해양과학기술원[DOI]
- 길현우, "태반성 성선 자극 호르몬 처리에 의한 복섬, Takifugu niphobles의 인공 산란유도", 수산해양교육연구, 29(2), pp.409~414 (2017), 한국수산해양교육학회
- 구인본, "Stimulation of Spermiation by Human Chorionic Gonadotropin and Carp Pituitary Extract in Grass Puffer, Takifugu niphobles", 발생과 생식, 19(4), pp.253~258 (2015), 한국발생생물학회
- Han KH, "Spawaning behavior and early life history of Takifugu pardalis (Teleostei: Tetraodontidae) in Korea", Kor J Ichthyol, 3, pp.181~189 (2001)
- Han KN, "Development of eggs, larvae and juveniles of the puffer, Takifugu rubripes reared in the laboratory", Japan J Ichthyol, 33, pp.186~194 (1999)
- Honma Y, "Maturation and spawning behavior of the puffer, Fugu niphobles, occurring on the coast of Sado Island in the Sea of Japan (a preliminary report)", Jpn J Ichthyol, 27, pp.129~138 (1980)
- Ignat'eva GM, "Temperature dependence of cleavage rates in carp, pike, and whitefish", Sov J Dev Biol, 5, pp.24~28 (1975)
- Jang SI, "Embryonic, larval and juvenile stages in yellow puffer, Takifugu obscurus", J Aquacult, 1, pp.11~18 (1996)
- 김봉석, "Temperature-dependent Index of Mitotic Interval (τ_0) for Chromosome Manipulation in Korean Rose Bitterling Rhodeus uyekii", Fisheries and Aquatic Sciences, 14(4), pp.429~433 (2011), 한국수산과학회
- 김동수, "복섬, Takifugu niphobles의 산란기 및 비산란기에 따른 성적이형 비교", 수산해양교육연구, 29(2), pp.447~452 (2017), 한국수산해양교육학회
- Masaomi H, "Gonadal development and fertility of triploid grass puffer, Takifugu niphobles induced by cold shock treatment", Mar Biotechnol, 15, pp.133~144 (2013)[DOI]
- Mims SD, "Induced meiotic gynogenesis of paddlefish, Polyodon spathula", J World Aquacult Soc, 28, pp.334~343 (1997)[DOI]
- Nozaki M, "Spawning habit of the puffer, Fugu niphobles (Jordanet et Snyder)", Zool Mag, 85, pp.156~168 (1976)
- Oh SH, "Spawning behavior and early life history of grass puffer, Takifugu niphobles", Kor J Ichthyol, 4, pp.236~243 (2000)
- 박인석, "Egg Development and Mitotic Interval (τ0) in Black Plaice, Pleuronectes obscurus (Herzenstein)", Fisheries and Aquatic Sciences, 13(4), pp.278~283 (2010), 한국수산과학회
- Park I-S, "Effect of starvation on the just hatched grass puffer, Takifugu niphobles larvae", JMLS, 2, pp.27~33 (2017)
- Park I-S, "Determination of the temperature dependent index of mitotic interval (<TEX>${\tau}_{0}$<", TEX>) for chromosome manipulation in far eastern catfish, Silurus asotus, 2001, pp.13, 85~88
- Saat T, "Duration of synchronous cleavage cycles and rate of development at different temperatures in the Baltic herring", J Fish Biol, 48, pp.658~663 (1996)[DOI]
- Shelton WL, "Determination of the developmental duration (<TEX>${\tau}_{0}$<", TEX>) for ploidy manipulation in carps, 1993, pp.45, 73~81
- Shelton WL, "A temperature-dependent index of mitotic interval (<TEX>${\tau}_{0}$<", TEX>) for chromosome manipulation in paddlefish and shovelnose sturgeon, 1997, pp.59, 229~234
- Thorgaard GH, "Fish Physiology. Vol. 9B", pp.405~434 (1983), Academic Press
- Thorgaard GH, "Population Genetics and Fishery Management", pp.319~331 (1987), University of Washington Press
- Thorgaard GH, "Polyploidy induced by heat shock in rainbow trout", Trans Amer Fish Soc, 110, pp.546~550 (1981)[DOI]
- Yoo GY, "Cytogenetic analysis of hybrid and hybrid triploid between river puffer, Takifugu obscures and tiger puffer, T. rubripes", Aquacult Res, 49, pp.637~650 (2018)[DOI]