Exogenous Exposure to Estradiol Benzoate or Flutamide at the Weaning Age Alters Expression of Connexin Isoforms in the Initial Segment of Male Rat
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Connexin (Cx) is a complex which allows direct communication between neighboring cells via exchange of signaling molecules and eventually leads to functional harmony of cells in a tissue. The initial segment (IS) is an excurrent duct of male reproductive tract and expression of numerous genes in the IS are controlled by androgens and estrogens. The effects of these steroid hormones on gene expression in the IS during postnatal development have not extensively examined. The present research investigated expressional modulation of Cx isoforms in the IS by exogenous exposure to estrogen agonist, estradiol benzoate (EB), or androgen antagonist, flutamide (Flu), at weaning age. Two different doses of EB or Flu were subcutaneously administrated in 21-day old of male rats, and expressional changes of Cx isoforms in the adult IS were analyzed by quantitative real-time PCR. Treatment of a low-dose EB (0.015 μg/kg body weight) resulted in an increased expression of Cx31 gene and a decreased expression of Cx37 gene. A high-dose EB (1.5 μg/kg body weight) treatment caused an increase of Cx31 gene expression. Increased levels of Cx30.3 and Cx40 transcripts were observed with a low-dose Flu (500 μg/kg body weight) treatment. Treatment of high-dose Flu (50 mg/kg body weight) led to expressional increases of Cx30.3, 40, and 43 genes. Our previous and present findings suggest differential responsiveness on gene expression of Cx isoforms in the IS by androgens and estrogens at different postnatal ages.
Abstract
Connexin (Cx) is a complex which allows direct communication between neighboring cells via exchange of signaling molecules and eventually leads to functional harmony of cells in a tissue. The initial segment (IS) is an excurrent duct of male reproductive tract and expression of numerous genes in the IS are controlled by androgens and estrogens. The effects of these steroid hormones on gene expression in the IS during postnatal development have not extensively examined. The present research investigated expressional modulation of Cx isoforms in the IS by exogenous exposure to estrogen agonist, estradiol benzoate (EB), or androgen antagonist, flutamide (Flu), at weaning age. Two different doses of EB or Flu were subcutaneously administrated in 21-day old of male rats, and expressional changes of Cx isoforms in the adult IS were analyzed by quantitative real-time PCR. Treatment of a low-dose EB (0.015 μg/kg body weight) resulted in an increased expression of Cx31 gene and a decreased expression of Cx37 gene. A high-dose EB (1.5 μg/kg body weight) treatment caused an increase of Cx31 gene expression. Increased levels of Cx30.3 and Cx40 transcripts were observed with a low-dose Flu (500 μg/kg body weight) treatment. Treatment of high-dose Flu (50 mg/kg body weight) led to expressional increases of Cx30.3, 40, and 43 genes. Our previous and present findings suggest differential responsiveness on gene expression of Cx isoforms in the IS by androgens and estrogens at different postnatal ages.
참고문헌 (24)
- Adamali HI, "Apical and narrow cells are distinct cell types differing in their structure, distribution, and functions in the adult rat epididymis", J Androl, 17, pp.208~222 (1996)
- Akingbemi BT, "Oestrogenic and antiandrogenic chemicals in the environment: Effects on male reproductive health", Ann Med, 33, pp.391~403 (2001)
- Carreau S, "Aromatase and estrogens in man reproduction: A review and latest advances", Adv Med Sci, 53, pp.139~144 (2008)
- Cooke PS, "Estrogen receptor expression in developing epididymis, efferent ductules, and other male reproductive organs", Endocrinology, 128, pp.2874~2879 (1991)
- Cosentino MJ, "Structure and function of the epididymis", Urol Res, 14, pp.229~240 (1986)
- Cyr DG, "Immunocytochemical localization and regulation of connexin 43 in the adult rat epididymis", Endocrinology, 137, pp.1474~1484 (1996)
- Dubé E, "Epidermal growth factor regulates connexin 43 in the human epididymis: role of gap junctions in azoospermia", Hum Reprod, 27, pp.2285~2296 (2012)
- Dufresne J, "Expression of multiple connexins in the rat epididymis indicates a complex regulation of gap junctional communication", Am J Physiol Cell Physiol, 284, pp.33~43 (2003)
- Goodenough DA, "Connexins, connexons, and intercellular communication", Annu Rev Biochem, 65, pp.475~502 (1996)
- 한수영, "The Expression Patterns of Connexin Isoforms in the Rat Caput Epididymis During Postnatal Development", 한국동물자원과학회지, 55(4), pp.249~255 (2013), 한국동물자원과학회
- Jones RC, "Changes in protein composition of the luminal fluids along the epididymis of the tammar, Macropus eugenii", J Reprod Fertil, 80, pp.193~199 (1987)
- Joseph A, "Estrogen, efferent ductules, and the epididymis", Biol Reprod, 84, pp.207~217 (2011)
- 이기호, "Differential Expression of Multiple Connexins in Rat Corpus and Cauda Epididymis at Various Postnatal Stages", 한국동물자원과학회지, 55(6), pp.521~530 (2013), 한국동물자원과학회
- 이기호, "Expressional Modulation of Connexin Isoforms in the Initial Segment of Male Rat treated with Estradiol Benzoate or Flutamide", 발생과 생식, 18(4), pp.293~300 (2014), 한국발생생물학회
- Meşe G, "Gap junctions: basic structure and function", J Invest Dermatol, 127, pp.2516~2524 (2007)
- Pointis G, "Gap junctional communication in the male reproductive system", Biochim Biophy Acta, 1719, pp.102~116 (2005)
- Robaire B, "Androgen action in the epididymis", J Androl, 32, pp.592~599 (2011)
- Robaire B, "The Physiology of Reproduction", pp.999~1080 (1988), Raven Press
- Robaire B, "Testis, Epididymis and Technologies in the Year 2000", pp.229~262 (2000), Springer
- Sar M, "Oestrogen receptor alpha and beta in rat prostate and epididymis", Andrologia, 32, pp.295~301 (2000)
- Hee-Jung Seo, "Expressional Profiling of Connexin Isoforms in the Initial Segment of the Male Reproductive Tract during Postnatal Development", Reproductive & Developmental biology, 34(2), pp.103~109 (2010), 한국동물번식학회
- St-Pierre N, "Neonatal hypothyroidism alters the localization of gap junctional protein connexin 43 in the testis and messenger RNA levels in the epididymis of the rat", Biol Reprod, 68, pp.1232~1240 (2003)
- Temple-Smith PD, "Development and use of surgical procedures to bypass selected regions of the mammalian epididymis: effects on sperm maturation", J Reprod Fertil Suppl, 53, pp.183~195 (1998)
- You L, "Androgen receptor expression in the testes and epididymides of prenatal and postnatal Sprague-Dawley rats", Endocrine, 9, pp.253~261 (1998)