Expressional Changes of Connexin Isoform Genes in the Rat Caput Epididymis Exposed to Flutamide or Estradiol Benzoate at the Early Postnatal Age
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Direct communication between neighboring cells through connexin (Cx)-based gap junction is a crucial biological manner to regulate functions of a tissue consisting of multi-cell types. The present research evaluated expressional changes of Cx isoforms in the caput epididymis of adult rat exposed to estradiol benzoate (EB) or flutamide (Flu) at the early postnatal age. A single subcutaneous administration of EB at a low-dose [0.015 g /kg body weight (BW)] or a high-dose (1.5 g/kg BW) or Flu at a low-dose (500 g/kg BW) or a high-dose (5 mg/kg BW) was performed to an animal at 1 week of age. Quantitative real-time PCR analysis was employed to determine expressional changes of Cx isoforms. The transcript levels of Cxs30.3 and 37 were decreased by a low-dose EB treatment, while decreases of Cxs31, 31.1, 32, 40, and 45 transcript levels were observed with a low-dose EB treatment. The treatment of a high-dose EB resulted in expressional reduction of Cxs30.3, 31, 31.1, 37, 40, 43, and 45. The Flu treatment at a low dose caused increases of Cxs26, 37, and 40 transcript levels but decreases of Cxs31.1, 43, and 45 transcript levels. Increases of Cxs30.3, 31, 37, and 40 mRNA amounts were induced by a high-dose Flu treatment. However, exposure to a high-dose Flu produced expressional decreases of Cxs31.1, 32, and 43 in the adult caput epididymis. These observations suggest that exposure to EB or Flu at the neonatal period could lead to aberrant expression of Cx isoforms in the adult caput epididymis.
Abstract
Direct communication between neighboring cells through connexin (Cx)-based gap junction is a crucial biological manner to regulate functions of a tissue consisting of multi-cell types. The present research evaluated expressional changes of Cx isoforms in the caput epididymis of adult rat exposed to estradiol benzoate (EB) or flutamide (Flu) at the early postnatal age. A single subcutaneous administration of EB at a low-dose [0.015 g /kg body weight (BW)] or a high-dose (1.5 g/kg BW) or Flu at a low-dose (500 g/kg BW) or a high-dose (5 mg/kg BW) was performed to an animal at 1 week of age. Quantitative real-time PCR analysis was employed to determine expressional changes of Cx isoforms. The transcript levels of Cxs30.3 and 37 were decreased by a low-dose EB treatment, while decreases of Cxs31, 31.1, 32, 40, and 45 transcript levels were observed with a low-dose EB treatment. The treatment of a high-dose EB resulted in expressional reduction of Cxs30.3, 31, 31.1, 37, 40, 43, and 45. The Flu treatment at a low dose caused increases of Cxs26, 37, and 40 transcript levels but decreases of Cxs31.1, 43, and 45 transcript levels. Increases of Cxs30.3, 31, 37, and 40 mRNA amounts were induced by a high-dose Flu treatment. However, exposure to a high-dose Flu produced expressional decreases of Cxs31.1, 32, and 43 in the adult caput epididymis. These observations suggest that exposure to EB or Flu at the neonatal period could lead to aberrant expression of Cx isoforms in the adult caput epididymis.
참고문헌 (17)
- Atanassova N, "Age-, cell- and region-specific immunoexpression of estrogen receptor alpha (but not estrogen receptor beta) during postnatal development of the epididymis and vas deferens of the rat and disruption of this pattern by neonatal treatment with diethylstilbestrol", Endocrinology, 142, pp.874~886 (2001)
- Cyr DG, "Immunocytochemical localization and regulation of connexin43 in the adult rat epididymis", Endocrinology, 137, pp.1474~1484 (1996)
- Dufresne J, "Expression of multiple connexins in the rat epididymis indicates a complex regulation of gap junctional communication", Am J Physiol Cell Physiol, 284, pp.C33~C43 (2003)
- Goodenough DA., "Gap junctions", Cold Spring Harb. Perspect. Biol, 1, pp.a003061~ (2009)
- Gorowska E, "Postnatal exposure to flutamide affects CDH1 and CTNNB1 gene expression in adult pig epididymis and prostate and alters metabolism of testosterone", Andrology, 2, pp.186~197 (2014)
- 한수영, "The Expression Patterns of Connexin Isoforms in the Rat Caput Epididymis During Postnatal Development", 한국축산학회지, 55(4), pp.249~255 (2013), 한국축산학회
- Lawrence TS, "Transmission of hormonal stimulation by cell-to-cell communication", Nature, 272, pp.501~506 (1978)
- 이기호, "Differential Expression of Multiple Connexins in Rat Corpus and Cauda Epididymis at Various Postnatal Stages", 한국축산학회지, 55(6), pp.521~530 (2013), 한국축산학회
- 이기호, "Modification of Gene Expression of Connexins in the Rat Corpus Epididymis by Estradiol Benzoate or Flutamide Exposure at the Early Neonatal Age", 발생과 생식, 19(2), pp.69~77 (2015), 한국발생생물학회
- 이기호, "Changes in Expression of Connexin Isoforms in the Caudal Epididymis of Adult Sprague-Dawley Rats exposed to Estradiol Benzoate or Flutamide at the Neonatal Age", 발생과 생식, 20(3), pp.237~245 (2016), 한국발생생물학회
- Meşe G, "Gap junctions: Basic structure and function", J Invest Dermatol, 127, pp.2516~2524 (2007)
- Putz O, "Neonatal low- and high-dose exposure to estradiol benzoate in the male rat: II. Effects on male puberty and the reproductive tract", Biol Reprod, 65, pp.1506~1517 (2001)
- Robaire B, "The Physiology of Reproduction", pp.999~1080 (1988), Raven Press
- Robaire B, "The Physiology of Reproduction", pp.1071~1148 (2006), Elsevier
- St-Pierre N, "Neonatal hypothyroidism alters the localization of gap junctional protein connexin 43 in the testis and messenger RNA levels in the epididymis of the rat", Biol Reprod, 68, pp.1232~1240 (2003)
- Valiunas V, "Connexin-specific cell-tocell transfer of short interfering RNA by gap junctions", J Physiol, 568, pp.459~468 (2005)
- You L, "Androgen receptor expression in the testes and epididymides of prenatal and postnatal Sprague-Dawley rats", Endocrine, 9, pp.253~261 (1998)