Expression of Wee1 Gene in the Ascidian, Halocynthia roretzi Embryo
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ABSTRACT : It is suggested that FGF/Ras/MEK signaling plays crucial roles in specification and cell division of the mesodermal precursor cells in ascidian embryos. To investigate how the number of cell division in tissue precursor cells is determined, we have characterized Wee1 homolog, Hr-Wee1 of the ascidian Halocynthia roretzi. We found that the Hr-Wee1 mRNA is expressed both maternally and zygotically. Maternal transcript is localized to the cytoplasm in the animal cells, while zygotic expression is seen in cells of the endoderm lineage from 32-cell to 110-cell stages. Zygotic in situ signal is detected in the A-line neural plate cells of neurulae, and in epidermal cells of the head region of tailbud embryos. Embryos treated with MEK signaling inhibitor showed a similar pattern to normal embryos in expression of Hr-Wee1. Therefore, it is likely that MEK signaling does not affect the maternal and zygotic expression of Hr-Wee1.
Abstract
ABSTRACT : It is suggested that FGF/Ras/MEK signaling plays crucial roles in specification and cell division of the mesodermal precursor cells in ascidian embryos. To investigate how the number of cell division in tissue precursor cells is determined, we have characterized Wee1 homolog, Hr-Wee1 of the ascidian Halocynthia roretzi. We found that the Hr-Wee1 mRNA is expressed both maternally and zygotically. Maternal transcript is localized to the cytoplasm in the animal cells, while zygotic expression is seen in cells of the endoderm lineage from 32-cell to 110-cell stages. Zygotic in situ signal is detected in the A-line neural plate cells of neurulae, and in epidermal cells of the head region of tailbud embryos. Embryos treated with MEK signaling inhibitor showed a similar pattern to normal embryos in expression of Hr-Wee1. Therefore, it is likely that MEK signaling does not affect the maternal and zygotic expression of Hr-Wee1.
참고문헌 (21)
- Aligue R, "Regulation of Schizosaccharomyces pombe Wee1 tyrosine kinase", J Biol Chem, 20, pp.13320~13325 (1997)
- Busino L, "Cdc25A phosphatase: combinatorial phosphorylation, ubiquitylation and proteolysis", Oncogene, 11, pp.2050~2056 (2004)
- Campbell SD, "Drosophila Wee1 kinase rescues fission yeast from mitotic catastrophe and phosphorylates Drosophila Cdc2 in vitro", Mol Biol Cell, 10, pp.1333~1347 (1995)
- Coleman TR, "Cdc2 regulatory factors", Curr Opin Cell Biol, 6, pp.877~882 (1994)
- Favata MF, "Identification of a novel inhibitor of mitogen-activated protein kinase kinase", J Biol Chem, 273, pp.18623~18632 (1998)
- Ferguson AM, "Normal cell cycle and checkpoint responses in mice and cells lacking Cdc25B and Cdc25C protein phosphatases", Mol Cell Biol, 7, pp.2853~2860 (2005)
- Hutchins JR, "Many fingers on the mitotic trigger: post-translational regulation of the Cdc25C phosphatase", Cell Cycle, 1, pp.41~45 (2004)
- Kawashima T, "MAGEST: Maboya gene expression patterns and sequence tags", Nucleic Acids Res, 1, pp.133~135 (2000)
- Kim GJ, "Role of the FGF and MEK signaling pathway in the ascidian embryo", Dev Growth Differ, 5, pp.521~533 (2001)
- Leise WF 3rd, "Multiple Cdk1 inhibitory kinases regulate the cell cycle during development", Dev Biol, 1, pp.156~173 (2002)
- Lew DJ, "Regulatory roles of cyclin dependent kinase phosphorylation in cell cycle control", Curr Opin Cell Biol, 6, pp.795~804 (1996)
- McGowan CH, "Human Wee1 kinase inhibits cell division by phosphorylating p34cdc2 exclusively on Tyr15", EMBO J, 1, pp.75~85 (1993)
- Miya T, "Functional analysis of an ascidian homologue of vertebrate Bmp-2", Bmp-4 suggests its role in the inhibition of neural fate specification, 1997, pp.24, 5149~5159
- Mueller PR, "Cell cycle regulation of a Xenopus Wee1-like kinase", Mol Biol Cell, 1, pp.119~134 (1995)
- Nishida H, "Cell lineage analysis in ascidian embryos by intracellular injection of a tracer enzyme. III. Up to the tissue restricted stage", Dev Biol, 2, pp.526~541 (1987)
- Nishida H, "Specification of embryonic axis and mosaic development in ascidians", Dev Dyn, 4, pp.1177~1193 (2005)
- Nurse P, "Universal control mechanism regulating onset of M-phase", Nature, 344, pp.503~508 (1990)
- Okamoto K, "The existence of two distinct Wee1 isoforms in Xenopus: implications for the developmental regulation of the cell cycle", EMBO J, 10, pp.2472~2484 (2002)
- Russell P, "Negative regulation of mitosis by wee1+, a gene encoding a protein kinase homolog", Cell, 4, pp.559~567 (1987)
- Saitou N, "The neighbor-joining method: a new method for reconstructing phylogenetic trees", Mol Biol Evol, 4, pp.406~425 (1987)
- 서형주, "멍게의 수압수용체세포 형성에서 FGF와 MEK 신호의 역할", 발생과 생식, 13(4), pp.291~296 (2009), 한국발생생물학회