Cinnamon Clownfish Amphiprion melanopus의 난발생과 자치어 변태에 미치는 먹이생물과 갑상선호르몬의 영향
Egg Development and Effects of Livefood and Thyroid Hormone on the Amphiprion melanopus Larvae
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Amphiprion melanopus의 난발생과 자치어의 형태학적 발달에 대하여 기초 조사를 실시하였고, 먹이생물과 갑상선호르몬이 자치어의 성장과 체색에 미치는 영향에 대하여 조사하였다. 수정 후, 부화까지 총 168시간이 소요되었다. 갓 부화 자어의 크기는 평균 3.5±0.3 ㎜였으며, 대부분의 자어는 부화 후 경과일 수에 따라 체색 변화(변태)를 나타내었다; DAH(days after hatching) 10: 체색 흑화, DAH 15～20: 3개의 흰 줄무늬 출현, DAH 30: 등과 꼬리지느러미의 흰 줄무늬가 사라지기 시작, DAH 90: 체색 주황색; 성어: 체색은 진한 계피색이며 머리에만 흰 줄무늬 남음. Artemia와 T. japonicus를 함께 공급한 실험구의 성장은 Artemia만 공급한 실험구보다 빨랐다. T. japonicus 만을 공급한 실험구의 지느러미 체색은 진하고 선명한 주황색이었다. 갑상선호르몬을 처리한 4 ppm구의 성장이 다른 실험구(0, 2, 6 ppm)보다 빨랐고 6 ppm구가 가장 느렸다. 본 연구의 결과, 주요 먹이생물로 알려져 있는 Artemia와 함께 T. japonicus를 공급함으로써 A. melanopus의 성장과 체색을 개선시킬 수 있었고, 갑상선호르몬 또한 변태 및 체색 형성에 직접적으로 관여할 것으로 사료된다.
Abstract
The aim of this study was to investigate the egg development and the color change of Amphiprion melanopus, by the live food and thyroid hormone. After fertilizing, it took 168 h until hatching. The size of hatching larvae were 3.5±0.3 ㎜, and some individuals had small yolk sac. As reaching the particular age, they underwent the color change (metamorphosis) - Days after hatching (DAH) 10: black, DAH 15～20: the 3 white stripes appear, DAH 30: the dorsal & the caudal white stripe begin to disappear. DAH 90: body color become red and orange, adult: cinnamon body color and a white stripe on head. - The group fed enriched Artemia with Tigriopus japonicus grew the faster than with only Artemia. The fin coloration of the one fed the only T. japonicus has a vivid red color and orange color. T3-treated group (4 ppm) grew the faster than the others (0, 2, 6 ppm). The size of the 6 ppm group was the smallest. The results suggest T. japonicus can be used as a supplement with rotifer and Artemia for the juvenile development and coloration. T3 is considered to be involved in the metamorphosis and color formation of Amphipirion sp.
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