miRNA-222 Modulates Differentiation of Mouse Embryonic Stem Cells
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
MicroRNAs (miRNAs) function as a key regulator of diverse cellular functions. To find out novel miRNAs that promote the differentiation of mouse embryonic stem cells (mESCs), we compared the miRNAs expression profiles of mESCs under self-renewal vs. differentiation states. We noticed that miR-222 was highly expressed during the differentiation of mESCs. Quantitative RT-PCR analysis revealed that expression of miR-222 was up-regulated during the embryonic bodies formation and retinoic acid -dependent differentiation. When miR-222 was suppressed by antogomiR-222,the differentiation of mESCs was delayed compared to control. Self-renewal marker expression or cell proliferation was not affected but the expression of lineage specific marker was suppressed by the treatment of miR-222 inhibitor during the differentiation of mESCs. Taken together, these results suggest that miR-222 functions to promote the differentiation of mESCs by regulating expression of differentiation related genes.
Abstract
MicroRNAs (miRNAs) function as a key regulator of diverse cellular functions. To find out novel miRNAs that promote the differentiation of mouse embryonic stem cells (mESCs), we compared the miRNAs expression profiles of mESCs under self-renewal vs. differentiation states. We noticed that miR-222 was highly expressed during the differentiation of mESCs. Quantitative RT-PCR analysis revealed that expression of miR-222 was up-regulated during the embryonic bodies formation and retinoic acid -dependent differentiation. When miR-222 was suppressed by antogomiR-222,the differentiation of mESCs was delayed compared to control. Self-renewal marker expression or cell proliferation was not affected but the expression of lineage specific marker was suppressed by the treatment of miR-222 inhibitor during the differentiation of mESCs. Taken together, these results suggest that miR-222 functions to promote the differentiation of mESCs by regulating expression of differentiation related genes.
참고문헌 (26)
- Acunzo M, "MiR-130a targets MET and induces TRAIL-sensitivity in NSCLC by downregulation miR-221 and 222", Oncogene Epub (2011)
- Barroso-delJesus A, "Embryonic stem cell-specific miR302- 367 cluster: human gene structure and functional characterization of its core promoter", Mol Cell Biol, 28, pp.6609~6619 (2008)
- Card DA, "Oct4", Sox2-regulated miR-302 targets cyclin D1 in human embryonic stem cells, 2008, pp.28, 6426~6438
- Cardinali B, "Microrna-221 and microrna- 222 modulate differentiation and maturation of skeletal muscle cells", PLoS One, 4, pp.7607~ (2009)
- Evans MJ, "Establishment in culture of pluripotential cells from mouse embryos", Nature, 292, pp.154~156 (1981)
- Fraichard A, "In vitro differentiation of embryonic stem cells into glial cells and functional neurons", J Cell Science, 108, pp.3181~3188 (1995)
- Gustavo T, "MicroRNAs in embryonic stem cell function and fate", Gene & Development, 24, pp.2732~2741 (2010)
- Junlin Q, "microRNAs regulate human embryonic stem cell division", Cell Cycle, 8, pp.3729~3741 (2009)
- Kanellopoulou C, "Dicer-deficient mouse embryonic stem cells are defective in differentiation and centromeric silencing", Genes Dev, 19, pp.489~501 (2005)
- Kathryn N, "MicroRNAs as regulators of differentiation and cell fate decisions", Cell Stem Cell, 7, pp.36~41 (2010)
- Kato M, "microRNAs: small molecules with big roles - C. elegans to human cancer", Biol Cell, 100, pp.71~81 (2008)
- 김영국, "Functional links between clustered microRNAs: suppression of cell-cycle inhibitors by microRNA clusters in gastric cancer", NUCLEIC ACIDS RESEARCH, 37, pp.1672~1681, OXFORD UNIVERSITY PRESS
- 이소담, "Ganglioside GT1b Mediates Neuronal Differentiation of Mouse Embryonic Stem Cells", 발생과 생식, 13(3), pp.155~161 (2009), 한국발생생물학회
- Lin CH, "Myc-regulated microRNAs attenuate embryonic stem cell differentiation", EMBO J, 28, pp.3157~3170 (2009)
- Marson A, "Connecting microRNA genes to the core transcriptional regulatory circuitry of embryonic stem cells", Cell, 134, pp.521~533 (2008)
- Murchison EP, "Characterization of Dicer-deficient murine embryonic stem cells", Proc Natl Acad Sci USA, 102, pp.12135~12140 (2005)
- Qi J, "microRNAs regulate human embryonic stem cell division", Cell Cycle, 8, pp.3729~3741 (2009)
- Quintavalle C, "miR-221", 222 overexpression in human glioblastoma increases invasiveness by targeting the protein phosphate PTPu, 2011, ~
- Shah MY, "MicroRNAs miR-221 and miR-222: a new level of regulation in aggressive breast cancer", Genome Med, 3, pp.56~ (2011)
- Silvia G, "NF-kB and c-Jun induce the expression of the oncogenic miR-221 and miR-222 in prostate carcinoma and glioblastoma cells", Nucleic Acids Research, 39, pp.3892~3902 (2011)
- Staszel T, "Role of microRNAs in endothelial cell pathophysiology", Pol Arch Med Wewn, 121, pp.361~367 (2011)
- Stinson S, "TRPS1 targeting by miR-221", 222 promotes the epithelial-to-mesenchymal transition in breast cancer, 2011, pp.4, 41~
- Tarantino C, "miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic stem cells", FASEB J, 24, pp.3255~3263 (2010)
- Tay Y, "MicroRNAs to Nanog, Oct4 and Sox2 coding regions modulate embryonic stem cell differentiation", Nature, pp.1124~1128 (2008)
- Thomson JA, "Embryonic stem cell lines derived from human blastocysts", Science, 282, pp.1145~1147 (1998)
- Tsunoda T, "Oncogenic KRAS regulates miR-200c and miR-221", 222 in a 3D-specific manner in colorectal cancer cells, 2011, pp.31, 2453~2459