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Epigenetic modification of retinoic acid-treated human embryonic stem cells

김혜민|김용성|한용만|정현섭|Han Chul Lee|Byung Lae Park|Mi Jin Jang|Seun-Young Kim|신형두
생화학분자생물학회 (Korean Society Of Biochemistry And Molecular Biology)|2010.12|BMB Reports|제43권 제12호|pp. 830-835

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참고문헌

키워드

DNA methylationGene expressionRetinoic acidEpigenetic modificationHuman embryonic stem cell
주제분류화학

초록

Epigenetic modification of the genome through DNA methylation is the key to maintaining the differentiated state of human embryonic stem cells (hESCs), and it must be reset during differentiation by retinoic acid (RA) treatment. A genome-wide methylation/gene expression assay was performed in order to identify epigenetic modifications of RA-treated hESCs. Between undifferentiated and RA-treated hESCs, 166 differentially methylated CpG sites and 2,013 differentially expressed genes were discovered. Combined analysis of methylation and expression data revealed that 19 genes (STAP2, VAMP8, C10orf26,WFIKKN1, ELF3, C1QTNF6, C10orf10, MRGPRF, ARSE, LSAMP,CENTD3, LDB2, POU5F1, GSPT2, THY1, ZNF574, MSX1,SCMH1, and RARB) were highly correlated with each other. The results provided in this study will facilitate future investigations into the interplay between DNA methylation and gene expression through further functional and biological studies.

Abstract

Epigenetic modification of the genome through DNA methylation is the key to maintaining the differentiated state of human embryonic stem cells (hESCs), and it must be reset during differentiation by retinoic acid (RA) treatment. A genome-wide methylation/gene expression assay was performed in order to identify epigenetic modifications of RA-treated hESCs. Between undifferentiated and RA-treated hESCs, 166 differentially methylated CpG sites and 2,013 differentially expressed genes were discovered. Combined analysis of methylation and expression data revealed that 19 genes (STAP2, VAMP8, C10orf26,WFIKKN1, ELF3, C1QTNF6, C10orf10, MRGPRF, ARSE, LSAMP,CENTD3, LDB2, POU5F1, GSPT2, THY1, ZNF574, MSX1,SCMH1, and RARB) were highly correlated with each other. The results provided in this study will facilitate future investigations into the interplay between DNA methylation and gene expression through further functional and biological studies.

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