Fus Expression Patterns in Developing Tooth
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Recently, the RNA/DNA-binding protein FUS, Fused in sarcoma, was shown to play a role in growth, differentiation, and morphogenesis in vertebrates. Because little is known about Fus, we investigated its expression pattern in murine tooth development. In situ hybridization of mouse mandibles at specific developmental stages was performed with a DIG-labeled RNA probe. During early tooth development, Fus was detected in the dental epithelium and dental mesenchyme at 11 days postcoitum (dpc) and 12 dpc. From 14 dpc, Fus was strongly expressed in the dental papilla and the cervical loop of the dental epithelium. At postnatal day 4 (PN4), Fus expression was observed in the odontoblasts, ameloblasts, the proliferation zone of the pulp, and the cervical loop. At PN14, the expression pattern of Fus was found to be maintained in the odontoblasts and the proliferation zone of the pulp. Furthermore, Fus expression was especially strong in the Hertwig’s epithelial root sheath (HERS). Therefore, this study suggests that Fus may play a role in the HERS during root development.
Abstract
Recently, the RNA/DNA-binding protein FUS, Fused in sarcoma, was shown to play a role in growth, differentiation, and morphogenesis in vertebrates. Because little is known about Fus, we investigated its expression pattern in murine tooth development. In situ hybridization of mouse mandibles at specific developmental stages was performed with a DIG-labeled RNA probe. During early tooth development, Fus was detected in the dental epithelium and dental mesenchyme at 11 days postcoitum (dpc) and 12 dpc. From 14 dpc, Fus was strongly expressed in the dental papilla and the cervical loop of the dental epithelium. At postnatal day 4 (PN4), Fus expression was observed in the odontoblasts, ameloblasts, the proliferation zone of the pulp, and the cervical loop. At PN14, the expression pattern of Fus was found to be maintained in the odontoblasts and the proliferation zone of the pulp. Furthermore, Fus expression was especially strong in the Hertwig’s epithelial root sheath (HERS). Therefore, this study suggests that Fus may play a role in the HERS during root development.
참고문헌 (25)
- Alappat AR, "Mouse pigpen encodes a nuclear protein whose expression is developmentally regulated during craniofacial morphogenesis", Dev Dyn, 228, pp.59~71 (2003)
- Alliegro MC, "A nuclear protein regulated during the transition from active to quiescent phenotype in cultured endothelial cells", Dev Biol, 174, pp.288~297 (1996)
- Alliegro MC, "Nuclear injection of anti-pigpen antibodies inhibits endothelial cell division", J Biol Chem, 277, pp.19037~19041 (2002)
- Aman P, "Expression patterns of the human sarcoma-associated genes FUS and EWS and the genomic structure of FUS", Genomics, 37(1), pp.1~8 (1996)
- Bertolotti A, "The N-terminal domain of human TAFII68 displays transactivation and oncogenic properties", Oncogene, 18(56), pp.8000~8010 (1999)
- Blank M, "Systematic evolution of a DNA aptamer binding to rat brain tumor microvesses, selective targeting of endothelial regulatory protein pigpen", J Biol Chem, 276, pp.16464~16468 (2001)
- Crozat A, "Fusion of CHOP to a novel RNA-binding protein in human myxoid liposarcoma", Nature, 363, pp.640~644 (1993)
- Dormann D, "Fused in sarcoma (FUS): An oncogene goes awry in neurodegeneration", Mol Cell Neurosci (2013)
- Eblaghie MC, "Interactions between FGF and Wnt signals and Tbx3 gene expression in mammary gland initiation in mouse embryos", WormBook, 14, pp.1~7 (2004)
- Gerber WV, "A role for the RNA-binding protein, hermes, in the regulation of heart development", Dev Biol, 247, pp.116~126 (2002)
- Hicks GG, "Fus deficiency in mice results in defective B-lymphocyte development and activation, high levels of chromosomal instability and perinatal death", Nat Genet, 24(2), pp.175~179 (2000)
- Hosoya A, "BMP4 signaling regulates formation of Hertwig’s epithelial root sheath during tooth development", Cell Tissue Res, 333, pp.503~509 (2008)
- 호소야 아키히로, "Expression of p63 during early craniofacial development of the mouse embryo", 발생과 생식, 13(2), pp.89~95 (2009), 한국발생생물학회
- Kwiatkowski Jr TJ, "Mutations in the FUS", TLS gene on chromosome 16 cause familial amyotrophic lateral sclerosis, 2009, pp.323, 1205~1208
- Morohoshi F, "Genomic structure of the human RBP56", hTAFII68 and FUS, Gene, pp.1998, 221(2)[DOI]
- Peng Y, "Maternal cold inducible RNA binding protein is required for embryonic kidney formation in Xenopus laevis", FEBS Lett, 482, pp.37~43 (2000)
- Rabbitts TH, "Fusion of the dominant negative transcription regulator CHOP with a novel gene FUS by translocation t(12;16) in malignant liposarcoma", Nat Genet, 4, pp.175~180 (1993)
- Tanaka H, "Cloning and expression of the quaking gene in the zebrafish embryo", Mech Dev, 69, pp.209~213 (1997)
- Thesleff I, "Epithelial-Mesenchymal signaling tooth morphogenesis", J Cell Sci, 116, pp.1647~1648 (2000)
- Thesleff I, "Signaling networks regulating dental development", Mech Dev, 67, pp.111~123 (1997)
- Vance C, "Mutations in FUS, an RNA processing protein, cause familial amyotrophic lateral sclerosis type 6", Science, 323, pp.1208~1211 (2009)
- Wakamatsu Y, "Sequential expression and role of Hu RNA-binding proteins during neurogenesis", Development, 124, pp.3449~3460 (1997)
- Wu L, "The 36-kilodalton embryonic-type cytoplasmic polyadenylation elementbinding protein in Xenopus laevis in ElrA, a member of the ELAV family of RNAbinding proteins", Mol Cell Biol, 17, pp.6402~6409 (1997)
- Zhang Y, "Chen YP(2000)A new function of BMP4; dual role for BMP4 in regulation of Sonic hedgehog expression in the mouse tooth germ", Development, 127, pp.1431~1443 (2000)
- Zinszner H, "Ron D(1997)TLS(FUS)binds RNA in vivo and engages in nucleocytoplasmic shuttling", J Cell Sci, 110, pp.1741~1750 (1997)