Temporal Aquaporin 11 Expression and Localization during Preimplantation Embryo Development
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Environmental conditions during early mammalian embryo development are critical and some adaptational phenomena are observed. However, the mechanisms underlying them remain largely masked. Previously, we reported that AQP5 expression is modified by the environmental condition without losing the developmental potency. In this study, AQP11 was examined instead. To compare expression pattern between in vivo and in vitro, we conducted quantitative RT-PCR and analyzed localization of the AQP11 by whole mount immunofluorescence. When the fertilized embryos were developed in the maternal tracts, the level of Aqp11 trascripts was decreased dramatically until 2-cell stage. Its level increased after 2-cell stage and peaked at 4-cell stage, but decreased again dramatically until morula stage. Its transcript level increased again at blastocyst stage. In contrast, the levels of Aqp11 transcript in embryos cultured in vitro were as follows. The patterns of expression were similar but the overall levels were low compared with those of embryos grown in the maternal tracts. AQP11 proteins were localized in submembrane cytoplasm of embryos collected from maternal reproductive tracts. The immune-reactive signals were detected in both trophectoderm and inner cell mass. However, its localization was altered in in vitro culture condition. It was localized mainly in the plasma membrane of the blastocysts contacting with external environment. The present study suggests that early stage embryo can develop successfully by themselves adapting to their environmental condition through modulation of the expression level and localization of specific genes like AQP11.
Abstract
Environmental conditions during early mammalian embryo development are critical and some adaptational phenomena are observed. However, the mechanisms underlying them remain largely masked. Previously, we reported that AQP5 expression is modified by the environmental condition without losing the developmental potency. In this study, AQP11 was examined instead. To compare expression pattern between in vivo and in vitro, we conducted quantitative RT-PCR and analyzed localization of the AQP11 by whole mount immunofluorescence. When the fertilized embryos were developed in the maternal tracts, the level of Aqp11 trascripts was decreased dramatically until 2-cell stage. Its level increased after 2-cell stage and peaked at 4-cell stage, but decreased again dramatically until morula stage. Its transcript level increased again at blastocyst stage. In contrast, the levels of Aqp11 transcript in embryos cultured in vitro were as follows. The patterns of expression were similar but the overall levels were low compared with those of embryos grown in the maternal tracts. AQP11 proteins were localized in submembrane cytoplasm of embryos collected from maternal reproductive tracts. The immune-reactive signals were detected in both trophectoderm and inner cell mass. However, its localization was altered in in vitro culture condition. It was localized mainly in the plasma membrane of the blastocysts contacting with external environment. The present study suggests that early stage embryo can develop successfully by themselves adapting to their environmental condition through modulation of the expression level and localization of specific genes like AQP11.
참고문헌 (32)
- Andrew J, "Regulation of blastocyst formation", Fron Bio, pp.d708~d730 (2001)
- Bell CE, "Mitogen-activated protein kinase (MAPK) pathways mediate embryonic responses to culture medium osmolarity by regulating aquaporin 3 and 9 expression and localization, as well as embyonic apoptosis", Hum Reprod, 24, pp.1373~1386 (2009)
- Borgnia M, "Cellular and molecular biology of the aquaporin water channels", Annual Rev Biochem, 68, pp.425~458 (1999)
- Calvanese L, "In silico study of human aquaporin AQP11 and AQP12 channels", Protein Sci, 22, pp.455~466 (2013)
- Fellous A, "Temperature influences histone methylation and mRNA expression of the Jmj-C histone-demethylase orghologues during the early development of the oyster Crassostrea gigas", Mar Genomics, 19, pp.23~30 (2015)
- Gluckman PD, "The role of developmental plasticity and epigenetics in human health", Birth Defects Res C Embryo Today, 93, pp.12~18 (2011)
- Gonen T, "The structure of aquaporins", Quarterly Rev Biophys, 39, pp.361~396 (2006)
- Gorelick DA, "Aquaporin-11: a channel protein lacking apparent transport function expressed in brain", BMC Biochem, 7, pp.14~ (2006)
- Hua Y, "Expression and significance of aquaporins during pregnancy", Front Biosci, 18, pp.1373~1383 (2013)
- Huang HF, "Funciton of aquaporins in female and male reproductive systems", Hum Reprod Updata, 12, pp.785~795 (2006)
- Ikeda M, "The NPC motif of aquaporin-11, unkike the NPA motif of known aquaporins, is essentai lfor full expression of molecular function", J Biol Chem, 286, pp.3342~3350 (2011)
- Ishibashi K, "Cloning and funcitonal expression of a new water channel abundantly expressed in the testis permeable to water, glycerol, and urea", J Biol Chem, 272, pp.20782~20789 (1997)
- Ishibashi K, "Cloning and identificaiton of a new member of water channel (AQP10) as an aquaglyceroporin", Biochemica et Biophysica Acta, 1576, pp.335~340 (2002)
- Ishibashi K, "The role of mammalian superaquaporins inside the cell", Biochim Biophys Acta, 1840, pp.1507~1512 (2014)
- Itoh T, "Identification of a novel aquaporin, AQP12, expressed in pancreatic acinar cells", Biochem Biophys Res Commun, 330, pp.832~838 (2005)
- Kumari SS, "Aquaporin 5 knockout mouse lens develops hyperglycemic cataract", Biochem Biophys Res Com, 441, pp.333~338 (2013)
- Larsen HS, "Aquaporin 11 in the developing mouse submanibular gland", Eur J Oral Sci, 118, pp.9~13 (2010)
- Ma T, "Defective secretion of saliva in transgenic mice lacking aquaporin-5 water channels", J Biol Chem, 274, pp.20071~20074 (1999)
- Madeira A, "Human aquaporin-11 is a water and glycerol channel and localized in the vicinity of lipid droplets in human adipocytes", Obesity, 22, pp.2010~2017 (2014)
- Magni F, "Proteomic knowledge of human aquaporins", Proteomics, 6, pp.5637~5649 (2006)
- Morishita Y, "Disruption of aquaporin-11 produces polycystic kidneys following vacuolization of the proximal tubule", Mol Cell Biol, 25, pp.7770~7779 (2005)
- Nong YQ, "The expression and distribution of aquaprin 3 in mouse embryos before and after vitrification", J Assist Reprod genet, 30, pp.601~606 (2013)
- Offenberg H, "mRNAs encoding aquaporins are present during murine preimplantation development", Mol Reprod Dev, 57, pp.323~330 (2000)
- Offenberg H, "Functional challenge affects aquaporin mRNA abundance in mouse blastocysts", Mol Reprod Dev, 71, pp.422~430 (2015)
- 박재원, "Adaptive Transition of Aquaporin 5 Expression and Localization during Preimplantation Embryo Development by In Vitro Culture", 발생과 생식, 18(3), pp.153~160 (2014), 한국발생생물학회
- Takahashi S, "The role of cysteine 227 in subcellular localization, water permeability, and multimerization of aquaporin-11", FEBS Open Bio, 4, pp.315~320 (2014)
- Watson AJ, "Regulation of blastocyst formation", Front Biosci, 6, pp.D708~D730 (2001)
- Xiong Y, "Expression of aquaporins in human embryos and potential role of AQP3 and AQP7 in preimplantation mouse embryo development", Cell Physiol Biochem, 31, pp.649~658 (2013)
- Yakata K, "Aquaporin-11 containing a divergent NPA motif has normal water channel activity", Biochim Biophys Acta, 1768, pp.688~693 (2007)
- Yakata K, "Water permeability and characterization of aquaporin-11", J Struct Biol, 174, pp.315~320 (2011)
- Yasui M, "Rapid gating and anion permeability of an intracellular aquaporin", Nature, 402, pp.184~187 (1999)
- Yeung CH, "Aquaporin AQP11 in the testis: molecular identity and association with the processing of residual cytoplsm of elongated spermatids", Reproduction, 139, pp.209~216 (2010)