The Expressional Pattern of Epididymal Protease Inhibitor (EPPIN) in the Male Syrian Hamsters
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The spermatogenesis is the process by which spermatozoa are generated in the testes. The spermatozoa travel male reproductive tract during which they meet many substances secreted from reproductive organs. One of the substances is epididymal protease inhibitor (EPPIN) that is involved in the post-testicular maturation including capability of fertilizing the eggs. The expression of EPPIN gene was investigated in various tissues of sexually mature and regressed male Syrian ham-sters by reverse transcription polymerase chain reaction (RT-PCR). The EPPIN gene was identified in the testis and epididy-mis of the male Syrian hamsters and compared to the genes reported previously. There was no expression of EPPIN gene in reproductively and completely regressed testes of Syrian hamster. These results suggest that the expressions of the EPPIN gene are associated with the reproductive capability in the Syrian hamsters.
Abstract
The spermatogenesis is the process by which spermatozoa are generated in the testes. The spermatozoa travel male reproductive tract during which they meet many substances secreted from reproductive organs. One of the substances is epididymal protease inhibitor (EPPIN) that is involved in the post-testicular maturation including capability of fertilizing the eggs. The expression of EPPIN gene was investigated in various tissues of sexually mature and regressed male Syrian ham-sters by reverse transcription polymerase chain reaction (RT-PCR). The EPPIN gene was identified in the testis and epididy-mis of the male Syrian hamsters and compared to the genes reported previously. There was no expression of EPPIN gene in reproductively and completely regressed testes of Syrian hamster. These results suggest that the expressions of the EPPIN gene are associated with the reproductive capability in the Syrian hamsters.
참고문헌 (31)
- Bian ZH, "Localization of epididymal protease inhibitor in adult rat and its transcription profile in testis during postnatal development", Asian J Androl, 11, pp.731~739 (2009)
- 최돈찬, "Potency of Melatonin in Living Beings", 발생과 생식, 17(3), pp.149~177 (2013), 한국발생생물학회
- 최돈찬, "Continuous Melatonin Attenuates the Regressing Activities of Short Photoperiod in Male Golden Hamsters", 발생과 생식, 17(2), pp.111~119 (2013), 한국발생생물학회
- 최돈찬, "The Impacts of Photoperiods on Hypothalamic Proteins in the Reproductive Activities of Golden Hamsters", 발생과 생식, 14(3), pp.185~197 (2010), 한국발생생물학회
- 최돈찬, "Neuroendocrine System in Seasonal Breeder: Focusing on the Reproductive Activity of Male Golden Hamster", 발생과 생식, 16(1), pp.1~8 (2012), 한국발생생물학회[DOI]
- Clauss A, "A locus on human chromosome 20 contains several genes expressing protease inhibitor domains with homology to whey acidic protein", Biochem J, 368, pp.233~242 (2002)
- Cohen DJ, "Evidence that human epididymal protein ARP plays a role in gamete fusion through complementary sites on the surface of the human egg", Biol Reprod, 65, pp.1000~1005 (2001)
- Dacheux JL, "Contribution of epididymal secretory proteins for spermatozoa maturation", Microsc Res Tech, 61, pp.7~17 (2003)
- Ding X, "Variants of the EPPIN gene affect the risk of idiopathic male infertility in the Han-Chinese population", Hum Reprod, 25, pp.1657~1665 (2010)
- Du Plessis SS, "Semen hyperviscosity: Causes, consequences, and cures", Front Biosci Elite Ed, 5, pp.224~231 (2013)
- Emami N, "Association between kallikreinrelated peptidases (KLKs) and macroscopic indicators of semen analysis: Their relation to sperm motility", Biol Chem, 390, pp.921~929 (2009)
- Esfandiari N, "Sem-inal hyperviscosity is associated with poor outcome of in vitro fertilization and embryo transfer: A prospective study", Fertil Steril, 90, pp.1739~1743 (2008)
- Jalkanen J, "Novel epididymal protease inhibitors with Kazal or WAP family domain", Biochem Biophys Res Commun, 349, pp.245~254 (2006)
- Mitra A, "Analysis of recombinant human semenogelin as an inhibitor of human sperm motility", Biol Reprod, 82, pp.489~496 (2010)
- Nixon B, "The identification of mouse sperm-surface associated proteins and characterization of their ability to act as decapacitation factors", Biol Reprod, 74, pp.275~287 (2006)
- O'Rand MG, "In-hibition of sperm motility in male macaques with EP055, a potential non-hormonal male contraceptive", PLoS ONE, 13, pp.e0195953~ (2018)
- O'Rand MG, "Non-hormonal male contraception: A review and development of an Eppin based contraceptive", Pharmacol Ther, 157, pp.105~111 (2016)
- O'Rand MG, "Inhibition of human sperm motility by contraceptive anti-Eppin antibodies from infertile male monkeys: Ef-fect on cyclic adenosine monophosphate", Biol Reprod, 80, pp.279~285 (2009)
- O'Rand MG, "Epididymal protein targets: A brief his-tory of the development of epididymal protease inhibi-tor as a contraceptive", J Androl, 32, pp.698~704 (2011)
- O'Rand MG, "Reversible immunocontraception in male monkeys immunized with Eppin", Science, 306, pp.1189~1190 (2004)
- O'Rand MG, "Eppin: An effective target for male contraception", Mol Cell Endocrinol, 250, pp.157~162 (2006)
- Paasch U, "Identification of in-creased amounts of eppin protein complex components in sperm cells of diabetic and obese individuals by dif-ference gel electrophoresis", Mol Cell Proteomics, 10, pp.M110.00718~ (2011)
- Reiter RJ, "Photoperiod: Its importance as an impel-ler of pineal and seasonal reproductive rhythms", Int J Biometeorol, 24, pp.57~63 (1980)
- Richardson RT, "Cloning and sequencing of human Eppin: A novel fam-ily of protease inhibitors expressed in the epididymis and testis", Gene, 270, pp.93~102 (2001)
- Silva EJR, "Interacting pro-teins on human spermatozoa: Adaptive evolution of the binding of smenogelin I to EPPIN", PLoS ONE, 8, pp.e82014~ (2013)
- Sivashanmugam P, "Characterization of mouse Eppin and a gene cluster of similar protease in-hibitors on mouse chromosome 2", Gene, 312, pp.125~134 (2003)
- Stetson MH, "The Pineal Gland", pp.109~153 (1984), Raven Press
- Stetson MH, "Effects of exog-enous and endogenous melatonin on gonadal function in hamsters", J Neural Transm, 21(Suppl), pp.55~80 (1986)
- Wang Z, "Association of Eppin with se-menogelin on human spermatozoa", Biol Reprod, 72, pp.1064~1070 (2005)
- Yenugu S, "Antimicrobial activity of human EPPIN, an androgen-regulated, sperm- bound protein with a whey acidic protein motif", Biol Reprod, 71, pp.1484~1490 (2004)
- Zhang X, "Correlation of epididymal protease inhibitor and fibronectin in human semen", PLoS ONE, 8, pp.e82600~ (2013)
