Blood-Testis Barrier and Sperm Delayed in the Cauda Epididymis of the Reproductively Regressed Syrian Hamsters
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
The Syrian (golden) hamsters are seasonal breeders whose reproductive functions are active in summer and inactive in winter. In experimental facility mimicking winter climate, short photoperiod (SP) induces gonadal regression. The blood-testis barrier (BTB) of the sexually involuted animals have been reported to be permeable, allowing developing germ cells to be engulfed or sloughed off the epithelium of the seminiferous tubules. The expressions of genes related to the tight junction composing of BTB were investigated in the reproductive active and inactive testes. Claudin-11, occludin, and junctional adhesion molecule (JAM) were definitely expressed in the active testes but not discernably detected in the inactive testes. And spermatozoa (sperm) were observed in the whole lengths of epididymides in the active testes. They were witnessed in only cauda region of the epididymides but not in caput and corpus regions in animals with the inactive testes. The results imply that the disorganization of BTB is associated with the testicular regression. The developing germ cells are swallowed into the Sertoli cells or travel into the lumen, as supported by the presence of the sperm delayed in the last region of the epididymis. These outcomes suggest that both apoptosis and desquamation are the processes that eliminate the germ cells during the regressing stage in the Syrian hamsters.
Abstract
The Syrian (golden) hamsters are seasonal breeders whose reproductive functions are active in summer and inactive in winter. In experimental facility mimicking winter climate, short photoperiod (SP) induces gonadal regression. The blood-testis barrier (BTB) of the sexually involuted animals have been reported to be permeable, allowing developing germ cells to be engulfed or sloughed off the epithelium of the seminiferous tubules. The expressions of genes related to the tight junction composing of BTB were investigated in the reproductive active and inactive testes. Claudin-11, occludin, and junctional adhesion molecule (JAM) were definitely expressed in the active testes but not discernably detected in the inactive testes. And spermatozoa (sperm) were observed in the whole lengths of epididymides in the active testes. They were witnessed in only cauda region of the epididymides but not in caput and corpus regions in animals with the inactive testes. The results imply that the disorganization of BTB is associated with the testicular regression. The developing germ cells are swallowed into the Sertoli cells or travel into the lumen, as supported by the presence of the sperm delayed in the last region of the epididymis. These outcomes suggest that both apoptosis and desquamation are the processes that eliminate the germ cells during the regressing stage in the Syrian hamsters.
참고문헌 (38)
- Andersen Berg K, "Seasonal changes in spermatogenic activity and in plasma levels of FSH, LH and testosterone, and the effect of immunization against inhibin in the male silver fox (Vulpes vulpes)", Int J Androl, 24, pp.284~294 (2001)
- Cheng CY, "The blood-testis barrier and its implications for male contraception", Pharmacol Rev, 64, pp.16~64 (2012)
- 최돈찬, "Potency of Melatonin in Living Beings", 발생과 생식, 17(3), pp.149~177 (2013), 한국발생생물학회
- 최돈찬, "Neuroendocrine System in Seasonal Breeder: Focusing on the Reproductive Activity of Male Golden Hamster", 발생과 생식, 16(1), pp.1~8 (2012), 한국발생생물학회[DOI]
- Dadhich RK, "Identification of live germ-cell desquamation as a major mechanism of seasonal testis regression in mammals : A study in the Iberian mole(Talpa occidentalis)", Biol Reprod, 88, pp.101~ (2013)
- Dadhich RK, "Role of apoptosis and cell proliferation in the testicular dynamics of seasonal breeding mammals : A study in the Iberian mole, Talpa occidentalis", Biol Reprod, 83, pp.83~91 (2010)
- de Kretser DM, "Spermatogenesis", Hum Reprod, 13(Suppl 1), pp.1~8 (1998)
- Elliott JA, "Circadian rhythms and photoperiodic time measurement in mammals", Fed Proc, 35, pp.2339~2346 (1976)
- Gaston S, "Photoperiodic control of hamster testis", Science, 158, pp.925~928 (1967)
- Gliki G, "Spermatid differentiation requires the assembly of a cell polarity complex downstream of junctional adhesion molecule-C", Nature, 431, pp.320~324 (2004)
- Hollenbach J, "Loss of connexin43in murine Sertoli cells and its effect on blood-testis barrier formation and dynamics", PLOS ONE, 13, pp.e0198100~ (2018)
- Geon Hyung Jeon, "The Effects of Daily Melatonin Gavage on Reproductive Activity in the Male Syrian Hamsters", 발생과 생식, 24(4), pp.263~275 (2020), 한국발생생물학회
- Jiménez R, "Circannual testis changes in seasonally breeding mammals", Sex Dev, 9, pp.205~215 (2015)
- 이지수, "The Effects of Cynomorium songaricum on the Reproductive Activity in Male Golden Hamsters", 발생과 생식, 17(1), pp.37~43 (2013), 한국발생생물학회
- Luaces JP, "Spermatogenesis is seasonal in the large hairy armadillo, Chaetophractus villosus(Dasypodidae, Xenarthra, Mammalia)", Reprod Fertil Dev, 25, pp.547~557 (2013)
- Luaces JP, "Loss of Sertoli-germ cell adhesion determines the rapid germ cell elimination during the seasonal regression of the seminiferous epithelium of the large hairy armadillo Chaetophractus villosus", Biol Reprod, 90, pp.48~ (2014)
- Massoud D, "Germ cell desquamation-based testis regression in a seasonal breeder, the Egyptian long-eared hedgehog, Hemiechinus auritus", PLOS ONE, 13, pp.e0204851~ (2018)
- Mazaud Guittot S, "Claudin 11 deficiency in mice results in loss of the Sertoli cell epithelial phenotype in the testis", Biol Reprod, 82, pp.202~213 (2010)
- Meng J, "Androgens regulate the permeability of the blood–testis barrier", Proc Natl Acad Sci USA, 102, pp.16696~16700 (2005)
- Merico V, "Sertoli–immature spermatids disengagement during testis regression in the armadillo", Reproduction, 157, pp.27~42 (2019)
- Morales E, "Apoptosis and molecular pathways in the seminiferous epithelium of aged and photoinhibited Syrian hamsters(Mesocricetus auratus)", J Androl, 28, pp.123~135 (2007)
- Morales E, "Proliferation and apoptosis in the seminiferous epithelium of photoinhibited Syrian hamsters(Mesocricetus auratus)", Int J Androl, 25, pp.281~287 (2002)
- Morita K, "Claudin 11", OSP based tight junctions of myelin sheaths in brain and Sertoli cells in testis, 1999, pp.145, 579~588
- Morrow CMK, "Claudin 5 expression in mouse seminiferous epithelium is dependent upon the transcription factor ets variant 5 and contributes to blood–testis barrier function", Biol Reprod, 81, pp.871~879 (2009)
- Mruk DD, "The mammalian blood-testis barrier : Its biology and regulation", Endocr Rev, 36, pp.564~591 (2015)
- Muñoz E, "Seasonal changes of the Leydig cells of viscacha (Lagostomus maximus maximus). A light and electron microscopy study", Tissue Cell, 29, pp.119~128 (1997)
- Pan J, "Expression of claudin 11 in a rat model of varicocele and its effects on the blood testis barrier", Mol Med Rep, 18, pp.5647~5651 (2018)
- Reiter RJ, "The pineal and its hormones in the control of reproduction in mammals", Endocr Rev, 1, pp.109~131 (1980)
- Saitou M, "Complex phenotype of mice lacking occludin, a component of tight junction strands", Mol Biol Cell, 11, pp.4131~4142 (2000)
- Seco-Rovira V, "Testicular histomorphometry and the proliferative and apoptotic activities of the seminiferous epithelium in Syrian hamster(Mesocricetus auratus)during regression owing to short photoperiod", Andrology, 3, pp.598~610 (2015)
- Silva ND, "Macrophages and dendritic cells in the post-testicular environment", Cell Tissue Res, 363, pp.97~104 (2016)
- Smith TB, "Mononuclear phagocytes rapidly clear apoptotic epithelial cells in the proximal epididymis", Andrology, 2, pp.755~762 (2014)
- Stetson MH, "The Pineal Gland", pp.109~153 (1984), Raven Press
- Stetson MH, "Effects of exogenous and endogenous melatonin on gonadal function in hamsters", J Neural Transm, 21, pp.S55~S80 (1986)
- Watson-Whitmyre M, "A mathematical method for estimating paired testes weight from in situ testicular measurements in three species of hamster", Anat Rec, 213, pp.473~476 (1985)
- Wen Q, "Regulation of bloodtestis barrier(BTB)dynamics, role of actin-, and microtubule-based cytoskeletons", Methods Mol Biol, 1748, pp.229~243 (2018)
- Young KA, "Photoperiod-induced testicular apoptosis in European starlings(Sturnus vulgaris)", Biol Reprod, 64, pp.706~713 (2001)
- Zhou W, "Characteristics of the epididymal luminal environment responsible for sperm maturation and storage", Front Endocrinol, 9, pp.59~ (2018)
