Genetic Distances of Rainbow Trout and Masu Salmon as Determined by PCR-Based Analysis
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초록
This study used a PCR-based genetic analysis platform to create a hierarchical polar dendrogram of Euclidean genetic distances for two salmonid species, Oncorhynchus mykiss (rainbow trout, RT) and Oncorhynchus masou (masu salmon, MS). The species were distantly related to other fish species based on PCR results from using the designed oligonucleotide primer series. Five oligonucleotide primers were used to generate 330 and 234 scorable fragments in the RT and MS populations, respectively. The DNA fragments ranged in size from approximately 50 bp to more than 2,000 bp. The bandsharing (BS) results showed that the RT population had a higher average BS value (0.852) than that for the MS population (0.704). The genetic distance between individuals supported the presence of adjacent affiliation in cluster I (RT 01–RT 11). The observation of a significant genetic distance between the two Oncorhynchus species verifies that this PCR-based technique can be a useful approach for individual- and population-based biological DNA investigations. The results of this type of investigation can be useful for species safekeeping and the maintenance of salmonid populations in the mountain streams of Korea.
Abstract
This study used a PCR-based genetic analysis platform to create a hierarchical polar dendrogram of Euclidean genetic distances for two salmonid species, Oncorhynchus mykiss (rainbow trout, RT) and Oncorhynchus masou (masu salmon, MS). The species were distantly related to other fish species based on PCR results from using the designed oligonucleotide primer series. Five oligonucleotide primers were used to generate 330 and 234 scorable fragments in the RT and MS populations, respectively. The DNA fragments ranged in size from approximately 50 bp to more than 2,000 bp. The bandsharing (BS) results showed that the RT population had a higher average BS value (0.852) than that for the MS population (0.704). The genetic distance between individuals supported the presence of adjacent affiliation in cluster I (RT 01–RT 11). The observation of a significant genetic distance between the two Oncorhynchus species verifies that this PCR-based technique can be a useful approach for individual- and population-based biological DNA investigations. The results of this type of investigation can be useful for species safekeeping and the maintenance of salmonid populations in the mountain streams of Korea.
참고문헌 (30)
- 백국기, "무지개송어, Oncorhynchus mykiss의 종묘생산에 관한 연구 - 미국산 무지개송어의 부화 및 자치어 성장", 한국양식학회지, 20(2), pp.85~89 (2007), 한국양식학회
- Cagigas ME, "Combined assessment of genetic variability in populations of brown trout(Salmo trutta L. )based on allozymes, microsatellites, and RAPD markers", Mar Biotechnol, 1, pp.286~296 (1999)
- Callejas C, "Identification of Spanish barbel species using the RAPD technique", J Fish Biol, 53, pp.208~215 (1998)
- S. W. Chenyambuga, "Genetic Characterization of Indigenous Goats of Sub-saharan Africa Using Microsatellite DNA Markers", Animal Bioscience, 17(4), pp.445~452 (2004), 아세아·태평양축산학회
- Gwakisa PS, "Characterization of zebu cattle breeds in Tanzania using random amplified polymorphic DNA markers", Anim Genet, 25, pp.89~94 (1994)
- Huang BX, "Analysis of genetic structure of blacklip abalone(Haliotis rubra)populations using RAPD, minisatellite and microsatellite markers", Mar Biol, 136, pp.207~216 (2000)
- M. S. Islam, "Genetic Analysis of Three River Populations of Catla catla (HAMILTON) Using Randomly Amplified Polymorphic DNA Markers", Animal Bioscience, 18(4), pp.453~457 (2005), 아세아·태평양축산학회
- Jeffreys AJ, "DNA fingerprints of dogs and cats", Anim Genet, 18, pp.1~15 (1987)
- 김종연, "Genetic Differences and DNA Polymorphism in Oyster (Crassostrea spp.) Analysed by PAPD-PCR", Genes & Genomics, 26(2), pp.123~134 (2004), 한국유전학회
- 김기현, "해수 순치 무지개 송어(Oncorhynchus mykiss)의 식품학적 품질 특성", 한국수산과학회지, 47(2), pp.114~121 (2014), 한국수산과학회[DOI]
- Liu Z, "Inheritance of RAPD markers in channel catfish (Ictalurus punctatus), blue catfish (I. Furcatus) and their F1, F2 and backcross hybrids", Anim Genet, 29, pp.58~62 (1998)
- Mamuris Z, "Taxonomic relationships between four species of the Mullidae family revealed by three genetic methods : Allozymes, random amplified polymorphic DNA and mitochondrial DNA", J Fish Biol, 55, pp.572~587 (1999)
- McCormack GC, "Comparative analysis of two populations of the brittle star Amphiura filiformis(Echinodermata : Ophiuroidae)with different life history strategies using RAPD markers", Mar Biotechnol, 2, pp.100~106 (2000)
- Muchmore ME, "Direct sequencing of genomic DNA for characterization of a satellite DNA in five species of Eastern Pacific abalone", Mol Mar Biol Biotech, 7, pp.1~6 (1998)
- Myoung JG, "Morphological study of Oncorhynchus spp. (Pisces : Salmonidae)in Korea-IV. Comparison of morphological characters of chum salmon O. keta, masu salmon O. masou and rainbow trout O. mykiss", Korean J Ichthyol, 5, pp.96~112 (1993)
- Myoung JG, "Morphological study of Oncorhynchus spp. in Korea-V. Comparison of skeletal characters of chum salmon O. keta, masu salmon O. masou and rainbow trout O. mykiss", J Korean Fish Soc, 29, pp.208~229 (1996)
- 오현, "Genetic Distances of Three Mollusk Species Investigated by PCR Analysis", 발생과 생식, 18(1), pp.43~49 (2014), 한국발생생물학회
- Su Young Park, "Genetic Differences and Variations in Slipper Lobster (Ibacus ciliatus) and Deep Sea Lobster (Puerulus sewelli) Determined by RAPD Analysis", Genes & Genomics, 27(4), pp.307~317 (2005), 한국유전학회
- Partis L, "Identification of fish species using random amplified polymorphic DNA(RAPD)", Mol Cell Probes, 10, pp.435~441 (1996)
- Sohn SG, "Studies on viral disease of masu salmon, Oncorhynchus masou-II Isolation of infectious hematopoietic necrosis virus from masu salmon fry", J Fish Pathol, 6, pp.87~92 (1993)
- Young-Jae Song, "Genetic Differences of Three Pollicipes mitella Populations Identified by PCR Analysis", 발생과 생식, 17(3), pp.199~205 (2013), 한국발생생물학회
- Tassanakajon A, "Genetic structure in wild populations of black tiger shrimp(Penaeus monodon)using randomly amplified polymorphic DNA analysis", J Mar Biotechnol, 6, pp.249~254 (1998)
- Yoke-Kqueen C, "Random amplified polymorphic DNA analysis of genetically modified organisms", J Biotechnol, 127, pp.161~166 (2006)
- Yoon JM, "Ultrastructural change of osmoregulatory cells during seawater adaptation in rainbow trout (Oncorhynchus mykiss)", Korean J Ichthyol, 12, pp.111~117 (2002)
- 윤종만, "Genetic Distances of Paralichthys olivaceus Populations Investigated by PCR", 발생과 생식, 22(3), pp.283~288 (2018), 한국발생생물학회
- Jong-ManYoon, "Genetic Differences between Cultured and Wild Penaeid Shrimp (Penaeus chinensis) Populations Analysed by RAPD-PCR", Genes & Genomics, 25(1), pp.4~32 (2003), 한국유전학회
- Yoon JM, "Wide marsh clam(Corbicula spp)populations from three sites analysed by RAPD-PCR-AGE", Bull Electrochem, 19, pp.337~348 (2003)
- Jong-Man Yoon, "Genetic Similarity and Variation in the Cultured and Wild Crucian Carp (Carassius carassius) Estimated with Random Amplified Polymorphic DNA", Animal Bioscience, 15(4), pp.470~476 (2002), 아세아·태평양축산학회
- Jong-Man Yoon, "Genetic Differences within and between Populations of Korean Catfish (S. asotus) and Bullhead (P. fulvidraco) Analysed by RAPD-PCR", Animal Bioscience, 17(8), pp.1053~1061 (2004), 아세아·태평양축산학회
- Zhou L, "Analysis of genetic heterogeneity among five gynogenetic clones of silver crucian carp, Carassius auratus gibelio Bloch, based on detection of RAPD molecular markers", Cytogenet Cell Genet, 88, pp.133~139 (2000)