PCR Analysis for Genetic Distances of Two Charybdis Crab Populations
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Genomic DNA (gDNA) set apart from two populations of Korean Charybdis crab (Charybdis japonica) was augmented by PCR experiments. The five oligonucleotides primers (ONTprimers) were spent to yield the number of unique loci shared to each crab population (ULSECP) and number of loci shared by the two crab populations (LSTCP). 305 fragments (FRAGs) were identified in the Charybdis crab population A (CCPA), and 344 in the Charybdis crab population B (CCPB): 44 number of ULSECP (14.43%) in the CCPA and 110 (31.98%) in the CCPB. 44 number of LSTCP, with an average of 8.8 per primer, were detected in the two crab populations. The bandsharing (BS) value between entity’s no. 01 and no. 10 was the lowest (0.371) between the two CCPs. The average bandsharing (ABS) values of individuals in the CCPA (0.575±0.014) were lesser than in those originated from the CCPB (0.705±0.011) (p < 0.05). The polar hierarchical dendrogram (PHD) achieved by the five ONT-primers denotes three genetic clusters (GCs): cluster I (CHARYBCRAB 01, 04, 05, 06, and 08), cluster II (CHARYBCRAB 02, 03, 07, 09, 10, and 11) and cluster III (CHARYBCRAB 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, and 22). The shortest genetic distance (GD) displaying significant molecular difference (MD) was between individuals CHARYBCRAB no. 18 and CHARYBCRAB no. 17 (0.055).
Abstract
Genomic DNA (gDNA) set apart from two populations of Korean Charybdis crab (Charybdis japonica) was augmented by PCR experiments. The five oligonucleotides primers (ONTprimers) were spent to yield the number of unique loci shared to each crab population (ULSECP) and number of loci shared by the two crab populations (LSTCP). 305 fragments (FRAGs) were identified in the Charybdis crab population A (CCPA), and 344 in the Charybdis crab population B (CCPB): 44 number of ULSECP (14.43%) in the CCPA and 110 (31.98%) in the CCPB. 44 number of LSTCP, with an average of 8.8 per primer, were detected in the two crab populations. The bandsharing (BS) value between entity’s no. 01 and no. 10 was the lowest (0.371) between the two CCPs. The average bandsharing (ABS) values of individuals in the CCPA (0.575±0.014) were lesser than in those originated from the CCPB (0.705±0.011) (p < 0.05). The polar hierarchical dendrogram (PHD) achieved by the five ONT-primers denotes three genetic clusters (GCs): cluster I (CHARYBCRAB 01, 04, 05, 06, and 08), cluster II (CHARYBCRAB 02, 03, 07, 09, 10, and 11) and cluster III (CHARYBCRAB 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, and 22). The shortest genetic distance (GD) displaying significant molecular difference (MD) was between individuals CHARYBCRAB no. 18 and CHARYBCRAB no. 17 (0.055).
참고문헌 (36)
- Araneda C, "Identification of a dominant SCAR marker associated with colour traits in coho salmon(Oncorhynchus kisutch)", Aquaculture, 247, pp.67~73 (2005)
- Archak S, "Comparative assessment of DNA fingerprinting techniques(RAPD, ISSR and AFLP)for genetic analysis of cashew(Anacardium occidentale L. )accessions of India", Genome, 46, pp.362~369 (2003)
- Bernardet JF, "Polyphasic study of Chryseobacterium strains isolated from diseased aquatic animals", Syst Appl Microbiol, 28, pp.640~660 (2005)
- Diaz-Jaimes P, "Allozyme and RAPD variation in the eastern Pacific yellowfin tuna(Thunnus albacares)", Fish Bull, 101, pp.769~777 (2003)
- Dixon BA, "Genetic characterization of populations of the ectoparasitic caligid, Lepeophtheirus salmonis(Krøyer 1837)using randomly amplified polymorphic DNA", Aquac Res, 35, pp.730~741 (2004)
- Esselman EJ, "RAPD marker diversity within and divergence among species of Dendroseris(Asteraceae : Lactuceae)", Am J Bot, 87, pp.591~596 (2000)
- García G, "Genetic differentiation among annual fishes of the genus Cynolebias(Cyprinodontiformes, Rivuluidae)in a biosphere reserve site from Uruguay", Environ Biol Fishes, 70, pp.247~256 (2004)
- Ghatak S, "Species identification of clinically important Aeromonas spp. by restriction fragment length polymorphism of 16S rDNA", Lett Appl Microbiol, 44, pp.550~554 (2007)
- Gelin A, "Species identification and ecological study of the genus Palaemonetes(Decapoda : Caridea)in the French Mediterranean", J Crustac Biol, 26, pp.124~133 (2006)
- Godhe A, "Comparison of three common molecular tools for distinguishing among geographically separated clones of the diatom Skeletonema marinoi Sarno et Zingone(Bacillariophyceae)", J Phycol, 42, pp.280~291 (2006)
- Ho CT, "Flavor constituents in enzyme hydrolysates from shore swimming crab and spotted shrimp", J Korean Soc Food Sci Nutr, 30, pp.787~795 (2001)
- 허만규, "해양 갑각류 민꽃게(Charybdis japonica) 집단의 형태학적 변이성", 생명과학회지, 16(4), pp.672~675 (2006), 한국생명과학회
- Huang BX, "Analysis of genetic structure of blacklip abalone(Haliotis rubra)populations using RAPD, minisatellite and microsatellite markers", Mar Biol, 136, pp.207~216 (2000)
- 조수근, "Genetic Distances between Tailfin Anchovy (Coilia nasus) Populations Analyzed by PCR", 발생과 생식, 25(1), pp.59~65 (2021), 한국발생생물학회
- 윤종만, "Genetic Variation in Geographic Crayfish (Cambaroides similis) Populations", 한국어병학회지, 19(2), pp.141~153 (2006), 한국어병학회
- 강서경, "Geographic Variations of Three Fulvia mutica Populations", The Korean Journal of Malacology, 29(3), pp.163~169 (2013), 한국패류학회[DOI]
- Kim DA, "Fishing mechanism of pots and their modification", Bull Korean Fish Soc, 20, pp.348~354 (1987)
- 김종연, "Genetic Differences and DNA Polymorphism in Oyster (Crassostrea spp.) Analysed by PAPD-PCR", Genes & Genomics, 26(2), pp.123~134 (2004), 한국유전학회
- 김영식, "Genetic Distances in Two Gracilaria Species (Gracilariaceae, Rhodophyta) Identified by PCR Technique", 발생과 생식, 22(4), pp.393~402 (2018), 한국발생생물학회
- Koh MC, "Random amplified polymorphic DNA(RAPD)fingerprints for identification of red meat animal species", Meat Sci, 48, pp.275~285 (1998)
- McCormack GP, "Comparative analysis of two populations of the brittle star Amphiura filiformis(Echinodermata : Ophiuroidea)with different life history strategies using RAPD markers", Mar Biotechnol, 2, pp.100~106 (2000)
- Nagarajan M, "Genetic variability of Channa punctatus populations using randomly amplified polymorphic DNA", Aquac Res, 37, pp.1151~1155 (2006)
- Oh KS, "The character impact compounds of odor evolved from cooked shore swimming crab flesh", Korean J Fish Aquat Sci, 35, pp.122~129 (2002)
- 박일웅, "민꽃게(Charybdis japonica)장의 식품 성분", 수산해양교육연구, 20(1), pp.95~106 (2008), 한국수산해양교육학회
- 서인수, "민꽃게, Charybdis japonica (A. Milne-Edwards)의 섭식생태", 환경생물, 27(3), pp.297~305 (2009), 한국환경생물학회
- Upadhyay SK, "Genetic diversity of yellow grouper(Epinephelus awoara)determined by random amplified polymorphic DNA(RAPD)analysis", Fish Bull, 104, pp.638~642 (2006)
- Wang CH, "Phylogenetic relationships of ornamental(koi)carp, Oujiang color carp and long-fin carp revealed by mitochondrial DNA COII gene sequences and RAPD analysis", Aquaculture, 231, pp.83~91 (2004)
- Wasko AP, "Genetic monitoring of the Amazonian fish matrinchã(Brycon cephalus)using RAPD markers : Insights into supportive breeding and conservation programmes", J Appl Ichthyol, 20, pp.48~52 (2004)
- 연인자, "연평해역 민꽃게(Charybdis japonica) 유생의 출현 시기와 분포", 한국수산과학회지, 44(2), pp.162~166 (2011), 한국수산과학회
- Yoon JM, "Wide marsh clam(Corbicula spp. )populations from three sites analysed by RAPD-PCR-AGE", Bull Electrochem, 19, pp.337~348 (2003)
- Jong-ManYoon, "Genetic Differences between Cultured and Wild Penaeid Shrimp (Penaeus chinensis) Populations Analysed by RAPD-PCR", Genes & Genomics, 25(1), pp.4~32 (2003), 한국유전학회
- 윤종만, "Genetic Differences and Variation in Black Rockfish (Sebastes schlegeli) and Hwanghae Rockfish (S. koreanus) from the YellowSea", Genes & Genomics, 29(4), pp.437~445 (2007), 한국유전학회
- 윤종만, "Genetic Variations of Intra- and between-razor Clam Solen corneus Population Identified by PCR Analysis", 발생과 생식, 22(2), pp.193~198 (2018), 한국발생생물학회
- 윤종만, "Genetic Differences in Natural and Cultured River Pufferfish Populations by PCR Analysis", 발생과 생식, 24(4), pp.327~335 (2020), 한국발생생물학회
- 윤종만, "Analysis of Geographical Genetic Differences of Arkshell Populations in Korea", 발생과 생식, 25(2), pp.105~111 (2021), 한국발생생물학회
- 윤종만, "Genetic Distances for Intra- and Between-Group of Scapharca subcrenata from Yeosu of the Korea", 발생과 생식, 25(4), pp.305~311 (2021), 한국발생생물학회
