Correlation between Rab3A Expression and Sperm Kinematic Characteristics
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Ras-related (Rab) proteins, integral members of the monomeric G-protein family, play a pivotal role in regulating intracellular vesicular transport. These proteins contribute to male reproductive processes, specifically in acrosome formation, exocytosis, and sperm motility. Although a prior study indicated a correlation between Rab3A and sperm motility, including motion kinematic parameters such as mean dance, this association has only been explored within a limited sample size. Therefore, further verification is required to confirm the correlation between Rab3A and sperm motility parameters. In the present study, Rab3A expression, sperm motility, and motion kinematic parameters were analyzed in 150 boar spermatozoa. Additionally, correlations between Rab3A expression and sperm kinematic characteristics were evaluated statistically. The results revealed significant associations between Rab3A protein expression levels and various motion kinematic parameters. Specifically, Rab3A levels exhibited positive correlations with average path velocity (p<0.05), mean amplitude of lateral head displacement (p<0.05), and curvilinear velocity (p<0.01). Consequently, it is proposed that Rab3A protein plays a crucial role in male fertility through its correlation with sperm kinematic characteristics, making it a potential marker for sperm motility-related assessments.
Abstract
Ras-related (Rab) proteins, integral members of the monomeric G-protein family, play a pivotal role in regulating intracellular vesicular transport. These proteins contribute to male reproductive processes, specifically in acrosome formation, exocytosis, and sperm motility. Although a prior study indicated a correlation between Rab3A and sperm motility, including motion kinematic parameters such as mean dance, this association has only been explored within a limited sample size. Therefore, further verification is required to confirm the correlation between Rab3A and sperm motility parameters. In the present study, Rab3A expression, sperm motility, and motion kinematic parameters were analyzed in 150 boar spermatozoa. Additionally, correlations between Rab3A expression and sperm kinematic characteristics were evaluated statistically. The results revealed significant associations between Rab3A protein expression levels and various motion kinematic parameters. Specifically, Rab3A levels exhibited positive correlations with average path velocity (p<0.05), mean amplitude of lateral head displacement (p<0.05), and curvilinear velocity (p<0.01). Consequently, it is proposed that Rab3A protein plays a crucial role in male fertility through its correlation with sperm kinematic characteristics, making it a potential marker for sperm motility-related assessments.
참고문헌 (33)
- Aghazarian A, "Standard semen parameters vs. sperm kinematics to predict sperm DNA damage", World J Mens Health, 39, pp.116~122 (2021)
- Alory C, "Organization of the Rab-GDI", CHM superfamily : The functional basis for choroideremia disease, 2001, pp.2, 532~543
- Bae JW, "Prediction of male fertility using Ras-related proteins", J Anim Sci Technol, 64, pp.1024~1034 (2022)
- Bae JW, "Rasrelated proteins(Rab)are key proteins related to male fertility following a unique activation mechanism", Reprod Biol, 19, pp.356~362 (2019)
- Bae JW, "Ras-related proteins(Rab)play significant roles in sperm motility and capacitation status", Reprod Biol, 22, pp.100617~ (2022)
- Bongso TA, "Effect of sperm motility on human in vitro fertilization", Arch Androl, 22, pp.185~190 (1989)
- Bradford MM, "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding", Anal Biochem, 72, pp.248~254 (1976)
- Constantin G, "Leukocyte chemotaxis : From lysosomes to motility", Nat Immunol, 11, pp.463~464 (2010)
- Donnelly ET, "In vitro fertilization and pregnancy rates : The influence of sperm motility and morphology on IVF outcome", Fertil Steril, 70, pp.305~314 (1998)
- Goody RS, "Mechanisms of action of Rab proteins, key regulators of intracellular vesicular transport", Biol Chem, 398, pp.565~575 (2017)
- Goud B, "Rab proteins as major determinants of the Golgi complex structure", Small GTPases, 9, pp.66~75 (2018)
- Hinrichs K, "Hyperactivated sperm motility : Are equine sperm different?", J Equine Vet Sci, 32, pp.441~444 (2012)
- Hutagalung AH, "Role of Rab GTPases in membrane traffic and cell physiology", Physiol Rev, 91, pp.119~149 (2011)
- Iida H, "Identification of rab12 as a vesicleassociated small GTPase highly expressed in Sertoli cells of rat testis", Mol Reprod Dev, 71, pp.178~185 (2005)
- Jang SI, "Development of an optimal protocol to induce capacitation of boar spermatozoa in vitro", J Anim Reprod Biotechnol, 37, pp.285~291 (2022)
- Jung EJ, "Reproductive toxicity of ritonavir in male : Insight into mouse sperm capacitation", Reprod Toxicol, 114, pp.1~6 (2022)
- Kumar S, "Testis-specific GTPase(TSG) : An oligomeric protein", BMC Genomics, 17, pp.792~ (2016)
- Kwon WS, "Increased male fertility using fertility-related biomarkers", Sci Rep, 5, pp.15654~ (2015)
- Lau ASN, "Rab8B GTPase and junction dynamics in the testis", Endocrinology, 144, pp.1549~1563 (2003)
- Lin YH, "RAB10 interacts with the male germ cell-specific GTPase-activating protein during mammalian spermiogenesis", Int J Mol Sci, 18, pp.97~ (2017)
- Liu S, "Are Rab proteins the link between Golgi organization and membrane trafficking?", Cell Mol Life Sci, 69, pp.4093~4106 (2012)
- Moreno RD, "Vesicular traffic and Golgi apparatus dynamics during mammalian spermatogenesis : Implications for acrosome architecture", Biol Reprod, 63, pp.89~98 (2000)
- Nakamura Y, "Expression and intracellular localization of TBC1D9, a Rab GTPase-accelerating protein, in mouse testes", Exp Anim, 64, pp.415~424 (2015)
- Pereira-Leal JB, "The mammalian Rab family of small GTPases : Definition of family and subfamily sequence motifs suggests a mechanism for functional specificity in the Ras superfamily", J Mol Biol, 301, pp.1077~1087 (2000)
- Ramalho-Santos J, "Membrane trafficking machinery components associated with the mammalian acrosome during spermiogenesis", Exp Cell Res, 267, pp.45~60 (2001)
- Rodríguez-Gil JE, "Expression of the GM-CSF receptor in ovine spermatozoa : GM-CSF effect on sperm viability and motility of sperm subpopulations after the freezing–thawing process", Theriogenology, 67, pp.1359~1370 (2007)
- Shan MM, "The multiple roles of RAB GTPases in female and male meiosis", Hum Reprod Update, 27, pp.1013~1029 (2021)
- Smith CE, "Ultrastructural distribution of NADPase within the Golgi apparatus and lysosomes of mammalian cells", Prog Histochem Cytochem, 21, pp.1~120 (1990)
- Stenmark H, "Rab GTPases as coordinators of vesicle traffic", Nat Rev Mol Cell Biol, 10, pp.513~525 (2009)
- Suarez SS, "Hyperactivation enhances mouse sperm capacity for penetrating viscoelastic media", Biol Reprod, 46, pp.686~691 (1992)
- Vieira OV, "Rab3a and Rab10 are regulators of lysosome exocytosis and plasma membrane repair", Small GTPases, 9, pp.349~351 (2018)
- Wu YW, "Membrane targeting mechanism of Rab GTPases elucidated by semisynthetic protein probes", Nat Chem Biol, 6, pp.534~540 (2010)
- Yunes R, "Rab3A triggers the acrosome reaction in permeabilized human spermatozoa", Biol Reprod, 62, pp.1084~1089 (2000)