A Simple Confocal Microscopy-based Method for Assessing Sperm Movement
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In the field of reproductive medicine, assessment of sperm motility is a key factor for achieving successful artificial insemination, in vitro fertilization, or intracellular sperm injection. In this study, the motility of boar sperms was estimated using real-time imaging via confocal microscopy. To confirm this confocal imaging method, flagellar beats and whiplash- like movement angles were compared between fresh and low-temperature-preserved (17℃ for 24 h) porcine sperms. Low-temperature preservation reduced the number of flagellar beats from 11.0±2.3 beats/s (fresh sperm) to 5.7±1.8 beats/s and increased the flagellar bending angle from 19.8°±13.8° (fresh) to 30.6°±15.6°. These data suggest that sperm activity can be assessed using confocal microscopy. The observed motility patterns could be used to develop a sperm evaluation index and automated confocal microscopic sperm motility analysis techniques.
Abstract
In the field of reproductive medicine, assessment of sperm motility is a key factor for achieving successful artificial insemination, in vitro fertilization, or intracellular sperm injection. In this study, the motility of boar sperms was estimated using real-time imaging via confocal microscopy. To confirm this confocal imaging method, flagellar beats and whiplash- like movement angles were compared between fresh and low-temperature-preserved (17℃ for 24 h) porcine sperms. Low-temperature preservation reduced the number of flagellar beats from 11.0±2.3 beats/s (fresh sperm) to 5.7±1.8 beats/s and increased the flagellar bending angle from 19.8°±13.8° (fresh) to 30.6°±15.6°. These data suggest that sperm activity can be assessed using confocal microscopy. The observed motility patterns could be used to develop a sperm evaluation index and automated confocal microscopic sperm motility analysis techniques.
참고문헌 (32)
- Amann RP, "Can the fertility potential of a seminal sample be predicted accurately?", J Androl, 10, pp.89~98 (1989)
- Amann RP, "Computer-assisted sperm analysis (CASA): Capabilities and potential developments", Theriogenology, 81, pp.5~17 (2014)
- Amelar RD, "Semen analysis. An Office technique", Urology, 2, pp.607~611 (1973)
- Barros C, "Failure of zona penetration of hamster spermatozoa after prolonged preincubation in a blood serum fraction", J Reprod Fertil, 35, pp.89~95 (1973)
- Betancourt M, "Effect of two insecticides and two herbicides on the porcine sperm motility patterns using computer-assistedsemen analysis (CASA) in vitro", Reprod Toxicol, 22, pp.508~512 (2006)
- Bonilla E, "Identification and characterization of novel mammalian spermatogenic genes conserved from fly to human", Mol Hum Reprod, 14, pp.137~142 (2008)
- Brokaw CJ, "Calcium ion regulation of flagellar beat symmetry in reactivated sea urchin spermatozoa", Biochem Biophys Res Commun, 58, pp.795~800 (1974)
- Calhim S, "Postcopulatory sexual selection is associated with reduced variation in sperm morphology", PLoS ONE, 2(5), pp.e413~ (2007)
- Coetzee K, "Repeatability and variance analysis on multiple computer-assisted (IVOS) sperm morphology readings", Andrologia, 31, pp.163~168 (1999)
- Cummins JM, "The Sperm Cell", pp.395~398 (1982), The Hague
- David I, "Mass sperm motility is associated with fertility in sheep", Anim Reprod Sci, 161, pp.75~81 (2015)
- Eisenbach M, "Sperm chemotaxis", Rev Reprod, 4, pp.56~66 (1999)
- Farrel PB, "Quantification of bull sperm characteristics measured by computer-assisted sperm analysis (CASA) and the relationship to fertility", Theriogenology, 49, pp.871~879 (1998)
- Feldmann J, "Canine and feline endocrinology and reproduction", pp.718~733 (1996), W.B. Saunders
- Fetterlof PM, "Prediction of human sperm penetrating ability using computerized motion parameters", Mol Reprod Dev, 27, pp.326~331 (1990)
- Foote RH, "Some biases to consider in measuring fertility in artificial insemination", J Dairy Sci, 34, pp.490~ (1951)
- Holt C, "Objectively measured boar sperm motility parameters correlate with the outcomes of on-farm inseminations:Results of two fertility trials", J Androl, 18, pp.312~323 (1997)
- Immler S, "Pronounced within-individual plasticity in sperm morphometry across social environments", Evolution, 64, pp.1634~1643 (2010)
- King GJ, "A comparison of two methods for boar semen collection", J Anim Sci, 36, pp.563~565 (1973)
- Kozdrowski R, "Two protocols of cryopreservation of goat semen with the use of computer-assisted semen analysis system", Acta Veterinaria Brno, 76, pp.601~604 (2007)
- Lavara R. Moce E, "Do parameters of seminal quality correlate with the results of on-farm inseminations in rabbits?", Theriogenology, 64, pp.1130~1141 (2005)
- Menkveld R, "Measurement and significance of sperm morphology", Asian J Androl, 13, pp.59~68 (2011)
- Morales PK, "The relationship between the motility and morphology of spermatozoa in human semen", J Androl, 9, pp.241~247 (1988)
- Patel DV, "Contemporary in vivo confocal microscopy of the living human cornea using white light and laser scanning techniques: a major review", Clin Experiment Ophthalmol, 35, pp.71~88 (2007)
- Phillips DM, "Comparative analysis of mammalian sperm motility", J Cell Biol, 53, pp.561~573 (1972)
- Rikmenspoel R, "The tail movements of bull spermatozoa. Observations and model calculations", Biophys J, 5, pp.365~392 (1965)
- Smith KD, "Relation between indices of sperm analysis and pregnancy rate in infertile couples", Fertil Steril, 28, pp.1314~1319 (1977)
- Suttle JM, "Quantitative studies on variation in sperm head morphology of the hopping mouse, Notomys alexis", J Exp Zool, 247, pp.166~171 (1988)
- Verstegen J, "Computer assisted semen analyzers in andrology research and veterinary practice", Theriogenology, 57, pp.149~179 (2002)
- Vyt P, "Motility Assessment of porcine spermatozoa:A comparison of methods", Reprod Dom Anim, 39, pp.447~453 (2004)
- Ward PI, "Intraspecific variation in sperm size characters", Heredity, 80, pp.655~659 (1998)
- Yoshida M, "Sperm chemotaxis and regulation of flagellar movement by Ca2+", Mol Hum Repord, 17, pp.457~465 (2011)