Th2 Responses Elicited by Nasal Epithelial Cells Exposed to House Dust Mite Extract
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Objectives. Respiratory epithelial cells are the first site of interaction of allergens with the immune system. The aim of this study was to examine the effect of epithelial cells, which were stimulated with house dust mite (HDM) extracts, on the immune response of peripheral blood mononuclear cells (PBMCs). Methods. Primary nasal polyp epithelial cells were exposed to dermatophagoides pteronyssinus and dermatophagoides farina for 48 hr, and then the supernatant and cells were collected. After stimulation with HDM extract, the epithelial cells were co-cultured with PBMCs for 72 hr and then the supernatant was collected. We measured the interleukin (IL)-8 and granulocyte-macrophage colony stimulating factor to determine the activation of the epithelial cells. The tumor necrosis factor (TNF)-a, IL-5 and interferon-g were measured to evaluate the interaction between the epithelial cells and the PBMCs. The mRNA expression of intercellular adhesion molecule 1 (ICAM-1) was assessed using the anti-ICAM-1 antibody. Results. The HDM extracts activated the nasal epithelial cells and enhanced the expression of ICAM-1 mRNA and cell membrane ICAM-1. When the activated epithelial cells were co-cultured with PBMCs, the PBMCs produced lager amounts of TNF-a and IL-5. However the cytokine production was not inhibited by pretreatment with ICAM-1 antibody. Conclusion. HDM allergens induce allergic inflammation by activating nasal epithelial cells, yet the interaction of the epitheila cells and the PBMCs may not be associated with an ICAM-1 medicated mechanism.
Abstract
Objectives. Respiratory epithelial cells are the first site of interaction of allergens with the immune system. The aim of this study was to examine the effect of epithelial cells, which were stimulated with house dust mite (HDM) extracts, on the immune response of peripheral blood mononuclear cells (PBMCs). Methods. Primary nasal polyp epithelial cells were exposed to dermatophagoides pteronyssinus and dermatophagoides farina for 48 hr, and then the supernatant and cells were collected. After stimulation with HDM extract, the epithelial cells were co-cultured with PBMCs for 72 hr and then the supernatant was collected. We measured the interleukin (IL)-8 and granulocyte-macrophage colony stimulating factor to determine the activation of the epithelial cells. The tumor necrosis factor (TNF)-a, IL-5 and interferon-g were measured to evaluate the interaction between the epithelial cells and the PBMCs. The mRNA expression of intercellular adhesion molecule 1 (ICAM-1) was assessed using the anti-ICAM-1 antibody. Results. The HDM extracts activated the nasal epithelial cells and enhanced the expression of ICAM-1 mRNA and cell membrane ICAM-1. When the activated epithelial cells were co-cultured with PBMCs, the PBMCs produced lager amounts of TNF-a and IL-5. However the cytokine production was not inhibited by pretreatment with ICAM-1 antibody. Conclusion. HDM allergens induce allergic inflammation by activating nasal epithelial cells, yet the interaction of the epitheila cells and the PBMCs may not be associated with an ICAM-1 medicated mechanism.
참고문헌 (18)
- Mullol J, "Comparative study of the effects of different glucocorticoids on eosinophil survival primed by cultured epithelial cell supernatants obtained from nasal mucosa and nasal polyps", Thorax, 50(3), pp.270~274 (1995)
- Roca-Ferrer J, "Effect of topical anti-inflammatory drugs on epithelial cell induced eosinophil survival and GM-CSF secretion", Eur Respir J, 10(7), pp.1489~1495 (1997)
- Robinson C, "On the potential significance of the enzymatic activity of mite allergens to immunogenicity: clues to structure and function revealed by molecular characteriz", Clin Exp Allergy, 27(1), pp.10~21 (1997)
- Thomas WR, "Characterization and immunobiology of house dust mite allergens", Int Arch Allergy Immunol, 129(1), pp.1~18 (2002)
- King C, "Dust mite proteolytic allergens induce cytokine release from cultured airway epithelium", J Immunol, 161(7), pp.3645~3651 (1998)
- Herbert CA, "Augmentation of permeability in the bronchial epithelium by the house dust mite allergen Der p1", Am J Respir Cell Mol Biol, 12(4), pp.369~378 (1995)
- Mckay DM, "T cell-monocyte interactions regulate epithelial physiology in a coculture model of inflammation", Am J Physiol, 270(2), pp.C418~C428 (1996)
- Agace WW, "Tlymphocyte-epithelial-cell interaction: integrin aE(CD103)b7, LEEPCAM and chemokines", Curr Opin Cell Biol, 12(5), pp.563~568 (2000)
- Tovey ER, "Mite faeces are a major source of house dust allergens", Nature, 289(5798), pp.592~593 (1981)
- De Wit D, "Helper T-cell responses elicited by Der p1-pulsed dendritic cells and recombinant IL-12 in atopic and healthy subjects", J Allergy Clin Immunol, 105(2), pp.346~352 (2000)
- Pichavant M, "Asthmatic bronchial epithelium activated by the proteolytic allergen Der p 1 increases selective dendritic cell recruitment", J Allergy Clin Immunol, 115(4), pp.771~778 (2005)
- Asokananthan N, "House dust mite allergens induce proinflammatory cytokines from respiratory epithelial cells: The cytokine protease allergen, Der p 1, activated protease-activated receptor (PAR)-2 and inactivates PAR-1", J Immunol, 169(8), pp.4572~4578 (2002)
- Stacey MA, "The allergen Der p 1 induces NF-kB activation through interference with IkB a function in asthmatic bronchial epithelial cells", Biochem Biophys Res Commun, 236(2), pp.522~526 (1997)
- Shin SH, "Protease-dependent activatio of nasal polyp epithelial cells by airborne fungi leads to migration of eosinophils and neutrophils", Acta Otolaryngol, 126(12), pp.1286~1294 (2006)
- Rosseau S, "Monocyte migration through the alveolar epithelial barrier: adhesion molecule mechanisms and impact of chemokines", J Immunol, 164(1), pp.427~435 (2000)
- Perdue MH, "Intergrative immunophysiology in the intestinal mucosa", Am J Physiol, 267(2), pp.G151~G165 (1994)
- Kimura M, "Correlation of house dust mite-specific lymphocyte proliferation with IL-5 production, eosinophilila, and the severity of symptoms in infants with atopic dermatitis", J Allergy Clin Immunol, 101(1), pp.84~89 (1998)
- Nurse B, "PBMCs from both atopic asthmatic and nonatopic children show a TH2 cytokine response to house dust mite allergen", J Allergy Clin Immunol, 106(1), pp.84~91 (2000)