Evaluation of the Protective Effect of Beta Glucan on Amikacin Ototoxicity Using Distortion Product Otoacoustic Emission Measurements in Rats
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Objectives. This experimental study investigated the possible protective effect of beta glucans on amikacin ototoxicity. Methods. Thirty-eight rats with normal distortion product otoacoustic emissions (DPOAEs) were divided into four groups. Group K was the control group. Group A was injected intramuscularly (i.m.) with amikacin 600 mg/kg/day between days 1-15. Group AB was given beta glucan gavage 1 mg/kg/day on days 0-15 and given amikacin 600 mg/kg/day i.m. on days 1-15. Group B was administered only beta glucan gavage, 1 mg/kg/day, on days 0-15. The DPOAEs were elicited in different frequency regions between 2,003 and 9,515 Hz, as distortion product diagrams (DPgrams), before and after the medication was administered, in all groups, on days 1, 5, 10, and 15. Results. No significant changes in the DPgrams were observed in group K. In group A, significant deterioration was observed at the 8,003 and 9,515 Hz frequencies on day 10, and at the 3,991, 4,557, 5,660, 6,726, 8,003, and 9,515 Hz frequencies on day 15. For group AB, statistically significant deterioration was observed at the 2,824, 8,003, and 9,515 Hz frequencies on day 15. The results for group B showed a significant improvement of hearing at the 2,378, 2,824, 3,363, and 3,991 Hz frequencies on day 1, at the 3,363, 3,991, and 8,003 Hz frequencies on day 10, and at the 8,003 Hz frequency on day 15. Conclusion. This study suggests that amikacin-induced hearing loss in rats may be limited to some extent by concomitant use of beta glucan.
Abstract
Objectives. This experimental study investigated the possible protective effect of beta glucans on amikacin ototoxicity. Methods. Thirty-eight rats with normal distortion product otoacoustic emissions (DPOAEs) were divided into four groups. Group K was the control group. Group A was injected intramuscularly (i.m.) with amikacin 600 mg/kg/day between days 1-15. Group AB was given beta glucan gavage 1 mg/kg/day on days 0-15 and given amikacin 600 mg/kg/day i.m. on days 1-15. Group B was administered only beta glucan gavage, 1 mg/kg/day, on days 0-15. The DPOAEs were elicited in different frequency regions between 2,003 and 9,515 Hz, as distortion product diagrams (DPgrams), before and after the medication was administered, in all groups, on days 1, 5, 10, and 15. Results. No significant changes in the DPgrams were observed in group K. In group A, significant deterioration was observed at the 8,003 and 9,515 Hz frequencies on day 10, and at the 3,991, 4,557, 5,660, 6,726, 8,003, and 9,515 Hz frequencies on day 15. For group AB, statistically significant deterioration was observed at the 2,824, 8,003, and 9,515 Hz frequencies on day 15. The results for group B showed a significant improvement of hearing at the 2,378, 2,824, 3,363, and 3,991 Hz frequencies on day 1, at the 3,363, 3,991, and 8,003 Hz frequencies on day 10, and at the 8,003 Hz frequency on day 15. Conclusion. This study suggests that amikacin-induced hearing loss in rats may be limited to some extent by concomitant use of beta glucan.
참고문헌 (40)
- Priuska EM, "Mechanism and prevention of aminoglycoside ototoxicity: outer hair cells as targets and tools", Ear Nose Throat J, 76(3), pp.164~171 (1997)
- Roland PS, "Characteristics of systemic and topical agents implicated in toxicity of the middle and inner ear", Ear Nose Throat J, 82(Suppl 1), pp.3~8 (2003)
- Rizzi MD, "Aminoglycoside ototoxicity", Curr Opin Otolaryngol Head Neck Surg, 15(5), pp.352~357 (2007)
- Dai CF, "Uptake of fluorescent gentamicin by vertebrate sensory cells in vivo", Hear Res, 213(1-2), pp.64~78 (2006)
- Tran Ba Huy P, "Kinetics of gentamicin uptake and release in the rat: comparison of inner ear tissues and fluids with other organs", J Clin Invest, 77(5), pp.1492~1500 (1986)
- Dulon D, "Pharmacokinetics of gentamicin in the sensory hair cells of the organ of Corti: rapid uptake and long term persistence", C R Acad Sci III, 316(7), pp.682~687 (1993)
- Hashino E, "Endocytosis of aminoglycoside antibiotics in sensory hair cells", Brain Res, 704(1), pp.135~140 (1995)
- Marcotti W, "The aminoglycoside antibiotic dihydrostreptomycin rapidly enters mouse outer hair cells through the mechano-electrical transducer channels", J Physiol, 567(Pt 2), pp.505~521 (2005)
- Hirose K, "Dynamic studies of ototoxicity in mature avian auditory epithelium", Ann N Y Acad Sci, 884, pp.389~409 (1999)
- Hirose K, "Reactive oxygen species in chick hair cells after gentamicin exposure in vitro", Hear Res, 104(1-2), pp.1~14 (1997)
- Lopez-Gonzalez MA, "Aminoglycosides activate oxygen metabolites production in the cochlea of mature and developing rats", Hear Res, 136(1-2), pp.165~168 (1999)
- Priuska EM, "Formation of free radicals by gentamicin and iron and evidence for an iron", gentamicin complex, 1995, pp.50(11), 1749~1752
- Wu WJ, "Recent advances in understanding aminoglycoside ototoxicity and its prevention", Audiol Neurootol, 7(3), pp.171~174 (2002)
- Ross GD, "Therapeutic intervention with complement and beta-glucan in cancer", Immunopharmacology, 42(1-3), pp.61~74 (1999)
- Lee KY, "Macrophage activation by polysaccharide fraction isolated from Salicornia herbacea", J Ethnopharmacol, 103(3), pp.372~378 (2006)
- Kim MK, "Higher intracellular levels of uridinemonophosphate under nitrogen-limited conditions enhance metabolic flux of curdlan synthesis in Agrobacterium species", Biotechnol Bioeng, 62(3), pp.317~323 (1999)
- Janeway CA Jr, "Innate immune recognition", Annu Rev Immunol, 20, pp.197~216 (2002)
- Rao KM, "MAP kinase activation in macrophages", J Leukoc Biol, 69(1), pp.3~10 (2001)
- Brown GD, "Immune recognition: a new receptor for beta-glucans", Nature, 413(6851), pp.36~37 (2001)
- Tsiapali E, "Glucans exhibit weak antioxidant activity, but stimulate macrophage free radical activity", Free Radic Biol Med, 30(4), pp.393~402 (2001)
- Patchen ML, "Glucan: mechanisms involved in its “radioprotective” effect", J Leukoc Biol, 42(2), pp.95~105 (1987)
- Abdollahi M, "Increasing intracellular cAMP and cGMP inhibits cadmium-induced oxidative stress in rat submandibular saliva", Comp Biochem Physiol C Toxicol Pharmacol, 135C(3), pp.331~336 (2003)
- Feldman L, "Gentamicin-induced ototoxicity in hemodialysis patients is ameliorated by N-acetylcysteine", Kidney Int, 72(3), pp.359~363 (2007)
- Young SG, "Why are low-density lipoproteins atherogenic?", West J Med, 160(2), pp.153~164 (1994)
- Ichikawa I, "Renal antioxidant enzymes: their regulation and function", Kidney Int, 45(1), pp.1~9 (1994)
- Cheeseman KH, "An introduction to free radical biochemistry", Br Med Bull, 49(3), pp.481~493 (1993)
- Karaduman D, "The protective effect of beta-1,3-D-glucan on taxol-induced hepatotoxicity: a histopathological and stereological study", Drug Chem Toxicol, 33(1), pp.8~16 (2010)
- Cleary JA, "The effect of molecular weight and beta-1,6-linkages on priming of macrophage function in mice by (1,3)-beta-D-glucan", Immunol Cell Biol, 77(5), pp.395~403 (1999)
- Aquino TJ, "Ototoxicity and otoprotection in the inner ear of guinea pigs using gentamicin and amikacin: ultrastructural and functional aspects", Braz J Otorhinolaryngol, 74(6), pp.843~852 (2008)
- Conlon BJ, "Supplemental iron exacerbates aminoglycoside ototoxicity in vivo", Hear Res, 115(1-2), pp.1~5 (1998)
- Sinswat P, "Protection from ototoxicity of intraperitoneal gentamicin in guinea pig", Kidney Int, 58(6), pp.2525~2532 (2000)
- Song BB, "Iron chelators protect from aminoglycoside-induced cochleo- and vestibulo-toxicity", Free Radic Biol Med, 25(2), pp.189~195 (1998)
- Chen Y, "Aspirin attenuates gentamicin ototoxicity: from the laboratory to the clinic", Hear Res, 226(1-2), pp.178~182 (2007)
- Campbell KC, "Prevention of noise- and drug-induced hearing loss with D-methionine", Hear Res, 226(1-2), pp.92~103 (2007)
- Kopke RD, "Use of organotypic cultures of Corti’s organ to study the protective effects of antioxidant molecules on cisplatin-induced damage of auditory hair cells", Am J Otol, 18(5), pp.559~571 (1997)
- Fetoni AR, "Protective effects of alpha-tocopherol against gentamicin-induced oto-vestibulo toxicity: an experimental study", Acta Otolaryngol, 123(2), pp.192~197 (2003)
- Horiike O, "Effect of edaravone on streptomycin-induced vestibulotoxicity in the guinea pig", Laryngoscope, 114(9), pp.1630~1632 (2004)
- Asplund MS, "Protective effect of edaravone against tobramycin-induced ototoxicity", Acta Otolaryngol, 129(1), pp.8~13 (2009)
- McFadden SL, "M40403, a superoxide dismutase mimetic, protects cochlear hair cells from gentamicin, but not cisplatin toxicity", Toxicol Appl Pharmacol, 186(1), pp.46~54 (2003)
- 김형숙, "Stimulatory Effect of β-glucans on Immune Cells", Immune Network, 11(4), pp.191~195 (2011), 대한면역학회