Histological Effect of Basic Fibroblast Growth Factor on Chronic Vocal Fold Scarring in a Rat Model
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Objectives. Vocal fold scarring is one of the most challenging laryngeal disorders to treat and there are currently no consistently effective treatments available. Our previous studies have shown the therapeutic potential of basic fibroblast growth factor (bFGF) for vocal fold scarring. However, the histological effects of bFGF on scarred vocal fold have not been elucidated. The aim of this study was to examine the histological effects of bFGF on chronic vocal fold scarring. Methods. Sprague-Dawley rats were divided into phosphate buffered saline (sham) and bFGF groups. Unilateral vocal fold stripping was performed and the drug was injected into the scarred vocal fold for each group 2 months postoperatively. Injections were performed weekly for 4 weeks. Two months after the last injection, larynges were harvested and histologically analyzed. Results. A significant increase of hyaluronic acid was observed in the vocal fold of the bFGF group compared with that of the sham group. However, there was no remarkable change in collagen expression nor in vocal fold contraction. Conclusion. Significant increase of hyaluronic acid by local bFGF injection was thought to contribute to the therapeutic effects on chronic vocal fold scarring.
Abstract
Objectives. Vocal fold scarring is one of the most challenging laryngeal disorders to treat and there are currently no consistently effective treatments available. Our previous studies have shown the therapeutic potential of basic fibroblast growth factor (bFGF) for vocal fold scarring. However, the histological effects of bFGF on scarred vocal fold have not been elucidated. The aim of this study was to examine the histological effects of bFGF on chronic vocal fold scarring. Methods. Sprague-Dawley rats were divided into phosphate buffered saline (sham) and bFGF groups. Unilateral vocal fold stripping was performed and the drug was injected into the scarred vocal fold for each group 2 months postoperatively. Injections were performed weekly for 4 weeks. Two months after the last injection, larynges were harvested and histologically analyzed. Results. A significant increase of hyaluronic acid was observed in the vocal fold of the bFGF group compared with that of the sham group. However, there was no remarkable change in collagen expression nor in vocal fold contraction. Conclusion. Significant increase of hyaluronic acid by local bFGF injection was thought to contribute to the therapeutic effects on chronic vocal fold scarring.
참고문헌 (23)
- Gray SD., "Biomechanical and histologic observations of vocal fold fibrous proteins", Ann Otol Rhinol Laryngol, 109(1), pp.77~85 (2000)
- Thibeault SL., "Histologic and rheologic characterization of vocal fold scarring", J Voice, 16(1), pp.96~104 (2002)
- Chan RW., "The importance of hyaluronic acid in vocal fold biomechanics", Otolaryngol Head Neck Surg, 124(6), pp.607~614 (2001)
- Benninger MS., "Vocal fold scarring : current concepts and management", Otolaryngol Head Neck Surg, 115(5), pp.474~482 (1996)
- Woo P., "Diagnosis and treatment of persistent dysphonia after laryngeal surgery : a retrospective analysis of 62 patients", Laryngoscope, 104(9), pp.1084~1091 (1994)
- Rousseau B., "Characterization of vocal fold scarring in a canine model", Laryngoscope, 113(4), pp.620~627 (2003)
- Tateya T., "Histologic characterization of rat vocal fold scarring", Ann Otol Rhinol Laryngol, 114(3), pp.183~191 (2005)
- Tateya T., "Histological study of acute vocal fold injury in a rat model", Ann Otol Rhinol Laryngol, 115(4), pp.285~292 (2006)
- Ford CN., "Studies of injectable soluble collagen for vocal fold augmentation", Rev Laryngol Otol Rhinol(Bord), 108(1), pp.33~36 (1987)
- Charles N. Ford, "Autologous collagen vocal fold injection: A preliminary clinical study", The Laryngoscope, 105(9), pp.944~948 (1995), Wiley-Blackwell[DOI]
- Kriesel KJ., "Treatment of vocal fold scarring : rheological and histological measures of homologous collagen matrix", Ann Otol Rhinol Laryngol, 111(10), pp.884~889 (2002)
- Wexler DB., "Phonosurgical studies : fat-graft reconstruction of injured canine vocal cords", Ann Otol Rhinol Laryngol, 98(9), pp.668~673 (1989)
- Hirano S., "Growth factor therapy for vocal fold scarring in a canine model", Ann Otol Rhinol Laryngol, 113(10), pp.777~785 (2004)
- Hong HH., "Cytokine regulation of gingival fibroblast lysyl oxidase, collagen, and elastin", J Periodontol, 73(2), pp.145~152 (2002)
- Palmon A., "Basic fibroblast growth factor suppresses tropoelastin gene expression in cultured human periodontal fibroblasts", J Periodontal Res, 36(2), pp.65~70 (2001)
- Suehiro A., "Effects of basic fibroblast growth factor on rat vocal fold fibroblasts", Ann Otol Rhinol Laryngol, 119(10), pp.690~696 (2010)
- Welham NV., "A rat excised larynx model of vocal fold scar", J Speech Lang Hear Res, 52(4), pp.1008~1020 (2009)
- Hirano S., "Regenerative phonosurgical treatments for vocal fold scar and sulcus with basic fibroblast growth factor", Laryngoscope, 123(11), pp.2749~2755 (2013)
- Inagi K., "Physiologic assessment of botulinum toxin effects in the rat larynx", Laryngoscope, 108(7), pp.1048~1054 (1998)
- Suzuki T., "Laryngeal-respiratory kinematics are impaired in aged rats", Ann Otol Rhinol Laryngol, 111(8), pp.684~689 (2002)
- Hirano S., "Prevention of vocal fold scarring by topical injection of hepatocyte growth factor in a rabbit model", Laryngoscope, 114(3), pp.548~556 (2004)
- Heldin P., "Effect of growth factors on hyaluronan synthesis in cultured human fibroblasts", Biochem J, 258(3), pp.919~922 (1989)
- Hirano S., "Therapeutic potential of growth factors for aging voice", Laryngoscope, 114(12), pp.2161~2167 (2004)