DNA Marker를 이용한 벼멸구 저항성 품종의 분류
Classification of Cultivars Resistant to Brown Planthopper Using DNA Markers in Rice
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참고문헌
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초록
본 연구에서는 벼멸구 저항성과 연관된 DNA marker를 이용하여 벼멸구 저항성 품종들의 저항성원을 구명하고자 저항성 품종들에 대한 벼멸구 저항성 연관 marker들의 band pattern을 비교 분석하였다. Bph1과 연관된 4개의 DNA marker들 중 STS marker BpE18-3과 RFLP marker RG413 및 G258은 벼멸구 저항성 donor 품종 Mudgo, TKM6, ASD7간에 서로 다형성 band pattern을 나타내었다. 24개 벼 품종들에 대한 분류실험에서 BpE18-3과 RG413은 TKM6를 저항성원으로 하는 품종들과 그렇지 않은 품종들을 특이적으로 구분하였다. Bph1의 저항성원을 특이적으로 구분하고 있는 BpE18-3과 RG413은 MAS에 의한 벼멸구 저항성 유전자의 집적에 유용하게 활용될 수 있을 것이다.
Abstract
The DNA markers such as a STS marker, BpE18-3, and three RFLP markers, RG413, RG901, and G258 linked to Bph1 were employed to classify the source of resistance to brown planthopper (BHP) in rices. The markers, BpE18-3, RG413, and G258, showed distinct polymorphic patterns among the donors, Mudgo, TKM6, and ASD7 of BPH resistance. The marker BpE18-3 and RG413 among 4 markers tested clearly separated rice cultivars with resistant source derived from TKM 6. These markers will be applied to facilitate the pyramiding of resistant genes to BPH in rice breeding programme.
참고문헌 (15)
- Ha WH, "Detection of resistance gene to brown planttopper using RFLP and Isozyme marker in rice", 32(4), pp.319~322 (1999)
- Hirabayashi H, "RFLP mapping of Bph-1 in rice.", 45, pp.369~371 (1995)
- Huang Z, "Identification and mapping of two brown planthopper resistance genes in rice", 102, pp.929~934 (2001)
- Martinez CR, "Sources and inheritance to the brown planthopper", pp.264~267
- Murai H, "Construction of high-resolution linkage map of a rice brown planthopper resistance gene bph2", 103, pp.526~532 (2001)
- Nair S, "PCR-based DNA markers linked to a gall midge resistance gene has potential for marker-aided selection in rice", 92, pp.660~665 (1996)
- Pathak PK, "Selection of biotype populations 2 and 3 of Nilaparvata lugens by exposure to resistant rice varieties", pp.85~89, Heinrichs EA 1982
- Pejic I, "Comparative analysis of genetic similarity among maize inbred lines detected by RFLPs RAPDs, SSRs, and AFLPs, Theor Appl Genet", 97(g), pp.1248~1255 (1998)
- Ramalingam J, "STS and microsatellite marker-assisted selection for bacterial blight resistance and waxy genes in rice Oryza sativa L.", 127, pp.255~260 (2002), Euphytica
- Sharma PN, "Marker-assisted pyramiding of brown planthopper resistance genes Bph1 and Bph2 on rice chromosome 12", 140, pp.61~69 (2004), Hereditas
- Simioniuc D, "Genetic diversity and relationships among pea cultivars revealed by RAPDs and AFLPs", 121, pp.429~435 (2002)
- Taura S, "analysis of yams collected in the Yap islands and the Ulithi islands Kagoshima University Research Center for the Pacific Islands", 39, pp.77~81 (2003)
- Witcombe JR, "Resistance gene deployment strategies in cereal hybrids using marker-assisted selection", 112, pp.175~186 (2000)
- Xu XF, "RFLP-facilitated investigation of the quantitative resistance of rice to brown planthopper", 104, pp.248~253 (2002)
- Yeo US, "Effective screening methods and inheritance of resistance to brown planthopper in rice.", 27(4), pp.372~379 (1995)