벼멸구 저항성 유전자의 유묘기 저항성 반응
Response to Brown Planthopper Resistance Genes at Rice Seedling Stage
1
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
본 연구는 생태형 1에 대해 각각의 단일 저항성 유전자를가지고 있는 벼멸구 저항성 품종의 반응을 알아보고자 수행하였다. 실험에 사용한 벼멸구는 저항성 유전자가 없는 ‘남평벼’에서 10세대 이상을 키운 것을 사용하였다. 유묘저항성 검정은 4 ~ 5엽기에 개체 당 10마리의 벼멸구를 접종하였다. Bph1, Bph3, Bph18 유전자를 가진 품종은 매우 강한 저항성반응을, bph2, Bph6, bph7, Bph9을 가진 품종은 중도저항성을 보였다. ‘남평벼’와 함께 bph5, bph8를 가진 품종은 매우약한 반응을 보였다. 식이선호성은 전체 72시간동안 24시간마다 3반복 조사하였는데 72시간 후 ‘남평벼’와 ‘IR72’는 각각 690%, 0%를 나타냈다. 식이선호성과 유묘저항성 반응은특정 유전자에 대해 비슷한 그룹을 형성하였다. 접종 10일후벼멸구 생존율은 저항성 유전자를 가진 품종은 30% 이하 였지만 ‘남평벼’는 70% 였다. 본 실험 결과 Bph3와 Bph18 유전자는 벼멸구 저항성 품종을 육성하는데 매우 유용할 것으로 판단된다.
Abstract
This experiments were carried out to know the response to Brown Planthopper(BPH) resistance genes at rice seedling stage using Biotype 1 for develoment of resistant cultivars. Varieties with Bph1, Bph3 and Bph18 genes showed a very strong resistance response, Bph2, Bph6, bph7 and Bph9 genes exhibited moderate resistance. bph5 and bph8 gene retention varieties and Nampyeongbyeo showed a very weak sensitivity in response to BPH. After 72 hours, Nampyeong(no gene) and IR72(Bph3 gene) were showed a feed-preference 690% and 0%, respectively. Results of Antixenosis and seedling resistance response to BPH were grouped into similar by specific resistance genes. Ten days after inoculation, BPH survival rate of vareities with resistance genes were below 30%, whereas Nampyeongbyeo was more than 70%. The results showed that Bph3 and Bph18 genes are highly resistant response against BPH, these genes are very useful for improve the rice cultivars with various resistance genes.
참고문헌 (32)
- Abo ME, "Rice virus diseases:epidemiology and management strategies", J. Sustain. Agr, 11, pp.113~134 (1997)
- Alam SN, "Detection and analysis of QTLs for resistance to the brown planthopper, Nilaparvata lugens, in a doubled haploid rice population", Theor. Appl. Genet, 97, pp.1370~1379 (1998)
- Bing L, "Potential resistance of tricin in rice against brown planthopper Nilaparvata lugens (Stål)", Acta Ecol. Sin, 27, pp.1300~1307 (2007)
- Brar DS, "Planthoppers: New Threats to the Sustainability of Intensive Rice Production Systems in Asia", pp.401~409 (2009), International Rice Research Institute
- Cho CI, "Resistance aspects of tongil and japonica varieties to brwon planthopper (Nilaparvata lgens Stal.)", Res. Rept. RDA(R), 30, pp.1~6 (1988)
- Claridge MF, "The biotype concept and its application to insect pests of agriculture", Crop Prot, 2, pp.85~95 (1983)
- Cruz AP, "Aspects of brown planthopper adaptation to resistant rice varieties with the Bph3 gene", Entomol, 141, pp.245~257 (2011)
- Denno RF, "Planthoppers: Their Ecology and Management" (1994), Chapman and Hall
- Dupo ALB, "Planthoppers: New Threats to the Sustainability of Intensive Rice Production Systems in Asia", pp.3~156 (2009), International Rice Research Institute
- Fujita D, "Rice resistance to planthoppers and leafhoppers", Critical Reviews in Plnat Science, 32, pp.162~191 (2013)
- Study on host plant resistance, "Goh HG", Agri. Sci. Ins., RDA, 606~613 (1988)
- Hibino H., "Biology and epidemiology of rice viruses", Ann. Rev. Phytopathol, 34, pp.249~274 (1996)
- Horgan F., "Planthoppers:New Threats to the Sustainability of Intensive Rice Production Systems in Asia", pp.281~302 (2009), International Rice Research Institute
- Jena KK, "Current status of brown planthopper (BPH) resistance and genetics", Rice, 3, pp.161~171 (2010)
- Jena KK, "High-resolution mapping of a new brwon planthopper resistance gene, Bph18(t), and marker-assisted selection for BPH resistance in rice(Oryza sativa L.)", Theor. Appl. Genet, 112, pp.288~297 (2006)
- Ketipearachchi Y, "Adaptation of the brown planthopper, Nilaparvata lugens, to BPH resistant rice cultivars carrying bph8 or Bph9", Applied Entomologyand Zoology, 33, pp.497~505 (1998)
- Khush GS, "IR varieties and their impact", International Rice Research Institute, 163~ (2005)
- Kim MK, "Reactions of resistance to brown planthopper in Japonica rice cultivars", RDA. J. Crop Protec, 40, pp.10~15 (1998)
- 김우재, "분자표지를 이용한 Bph18 보유 벼멸구 저항성 자포니카 계통 육성", 한국국제농업개발학회지, 25(4), pp.378~384 (2013), 한국국제농업개발학회[DOI]
- 손재근, "벼멸구 저항성 여교잡 집단의 등숙특성 QTL 분석", 한국육종학회지, 37(5), pp.295~299 (2005), 한국육종학회
- Studies on the biotypes of brown planthopper (Nilaparvata lugens Stål)in Korea: 1. Distribution of brwon planthopper biotypes, "Lee JO", S·P·M·U, 42~46, pp.ORD 26-2 (1984)
- Loka RK, "Biochemical basis of resistance in rice cultivars to brown planthopper Nilaparvata lugens (Stål)", J. Entomol. Res, 28, pp.79~85 (2004)
- Ōya S, "Arrest-deterring effect of resistant parental lines of Japonica rice to immigrant brown planthopper, Nilaparvata lugens Stål (Hemiptera:Delphacidae), in the field", Appl. Entomol. Zool, 22, pp.237~239 (1987)
- Park YD, "Studies on the distribution of the brwon planthopper (Nilaparvata lugens Stål) biotypes migrated in the sourthern regions of Korea", Korean J. Appl. Entomol, 27, pp.63~67 (1988)
- Pathak PK, "Selection of biotype populations 2 and 3 of Nilaparvata lugens by exposure to resistant rice varieties", Environmental Entomology, 11, pp.85~90 (1982)
- Seo BY, "Planthoppers: New threats ot the sustainability of intensive rice production systems in Asia", pp.303~314 (2009), International Rice Research Institute
- 서정필, "분자육종법을 이용한 벼멸구 저항성 고품질 자포니카 벼 신품종 ‘안미’ 육성", 한국육종학회지, 46(2), pp.152~159 (2014), 한국육종학회[DOI]
- Seo JP, "Development of elite breeding lines conferring Bph18gene derived resistance to brown planthopper by marker-assisted selection and genome-wide background analysis in japonica rice(Oryza sativa L.).", Field Crops Research, 120, pp.215~222 (2011)
- Shin MS, "Genetic analysis for resistance to bacterial blight and brown planthopper in japonica rice cultivars bred in Korea", Ph. D., Dep. Of Agr. Bio. Gradu. Sch., pp.1990
- 신문식, "벼멸구 저항성 Bph18 유전자가 벼의 수량구성요소에 미치는 영향", 한국육종학회지, 43(1), pp.56~61 (2011), 한국육종학회
- Yeo US, "Effective screening methods and inheritance of resistance to brown planthopper(Nilaparvata lugens Stal.) in rice", Korean J. Breed. Sci, 27, pp.372~379 (1995)
- Zhang F, "Differential gene expression in response to brown plantopper feeding in rice", J. Plant Physiol, 161, pp.53~62 (2004)