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발근력이 향상된 사과 대목 M.26 형질전환체

The Apple Rootstock Transgenic M.26 (Malus pumila) with Enhanced Rooting Ability

조강희|김현란|권순일|허성|신일섭|김정희
한국육종학회 (The Korean Society of Breeding Science)|2009.12|한국육종학회지 (KOREAN JOURNAL OF BREEDING SCIENCE)|제41권 제4호|pp. 482-487

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참고문헌

주제분류농학

초록

Abstract

The apple rootstock M.26 (Malus pumila) is very popular apple rootstock with semi-dwarf habit and the trees on M.26 produce high quality fruit at a young age. Since it is prone to poor prop ability to soil, however, young trees require staking in windy locations. The rolC gene was introduced into M.26 by Agrobacterium tumefaciens LBA4404 harboring pBI121 to obtain its transformants with dwarfism and enhanced rooting ability. One regenerated transgenic line was confirmed by polymerase chain reaction (PCR) analysis and Southern blot analysis of genomic DNA for the existence of rolC gene. The characteristics of transgenic line in vitro were not significantly different from non-transgenic line except for the active root formation and lateral root number. The rolC transgenic line showed reduced stem length and increased root number in vitro. Rooting ability was examined in the isolated greenhouse after mound layering. Compared to non-transgenic M.26, rolC transgenic line showed significantly higher rooting ability. The transgenic line did not show any other observable variation in shoot phenotype compared with non-transgenic line excepting increased branching.

참고문헌 (24)

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