Relationship of Phenolic Compounds and Free-radical Scavenging Activity in Black and Red Rice Extract
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Total phenolic content, total anthocyanin content and antioxidant activity were analyzed from rice samples collected in Korea, Japan and China. The results showed that the total phenolic content and free-radical scavenging activity differed significantly in these rice lines. The correlation between content and activity was subsequently investigated. The results showed that in black rice, anthocyanin was the major phenolic component and that both phenolic content and anthocyanin content were closely correlated with free-radical scavenging activity. Reverse Phase High Performance Liquid Chromatography (RP-HPLC)data showed that cyanidin-3-O-glucoside and peonidin-3-O-glucoside composed about 90% of the total anthocyanin content in black rice and in red rice. In the red rice extract, the total phenolic content produced a high correlation coefficient with antioxidant activity but correlated very poorly with the total anthocyanin content. The OD458 and the OD500 values which represent the proanthocyanidin content of the rice extract, produced high correlation coefficients with antioxidant activity and total phenolic content. These results suggest that the OD458 and the OD500 values can be used to evaluate the quality of red rice. In addition,based on the data obtained, a competitive accumulation model of anthocyanin and proanthocyanidin in black and red rice was proposed.
Abstract
Total phenolic content, total anthocyanin content and antioxidant activity were analyzed from rice samples collected in Korea, Japan and China. The results showed that the total phenolic content and free-radical scavenging activity differed significantly in these rice lines. The correlation between content and activity was subsequently investigated. The results showed that in black rice, anthocyanin was the major phenolic component and that both phenolic content and anthocyanin content were closely correlated with free-radical scavenging activity. Reverse Phase High Performance Liquid Chromatography (RP-HPLC)data showed that cyanidin-3-O-glucoside and peonidin-3-O-glucoside composed about 90% of the total anthocyanin content in black rice and in red rice. In the red rice extract, the total phenolic content produced a high correlation coefficient with antioxidant activity but correlated very poorly with the total anthocyanin content. The OD458 and the OD500 values which represent the proanthocyanidin content of the rice extract, produced high correlation coefficients with antioxidant activity and total phenolic content. These results suggest that the OD458 and the OD500 values can be used to evaluate the quality of red rice. In addition,based on the data obtained, a competitive accumulation model of anthocyanin and proanthocyanidin in black and red rice was proposed.
참고문헌 (19)
- Aruoma O.I., "Low molecular proanthocyanidin dietary biofactor Oligonol: Its modulation of oxidative stress, bioefficacy, neuroprotection, food application and chemoprevention potentials", Biofactors, 27, pp.245~265 (2006)
- Finocchiaro F., "Characterization of antioxidant compounds of red and white rice and changes in total antioxidant capacity during processing", Mol Nutr Food Res, 51, pp.1006~1019 (2007)
- Finocchiaro F., "A study of biodiversity of flavonoid content in the rice caryopsis evidencing simultaneous accumulation of anthocyanins and proanthocyanidins in a black-grained genotype", J Cereal Sci, 51, pp.28~34 (2010)
- Furukawa T., "The Rc and Rd genes are involved in proanthocyanidin synthesis in rice pericarp", Plant J., 49, pp.91~102 (2006)
- Ichikawa H., "Antioxidant Activity of Anthocyanin Extract from Purple Black Rice", J Med Food, 4, pp.211~218 (2001)
- Ikegami A., "Identification of genes involved in proanthocyanidin biosynthesis of persimmon (Diospyros kaki) fruit", Plant Sci, 172, pp.1037~1047 (2007)
- Kang T.H., "Neuroprotective effects of the cyanidin-3-O-beta- D-glucopyranoside isolated from mulberry fruit against cerebral ischemia", Neurosci Lett, 391, pp.122~126 (2006)
- Bong Gyu Kim, "Anthocyanin Content in Rice Is Related to Expression Levels of Anthocyanin Biosynthetic Genes", Journal of Plant Biology(한국식물학회지), 50(2), pp.156~160 (2007), 한국식물학회
- Linda C. S., "Environmental Significance of Anthocyanins in Plant Stress Responses", Photochem. Photobiol, 70(1), pp.1~9 (1999)
- Ling W.H., "Red and black rice decrease atherosclerotic plaque formation and increase antioxidant status in rabbits", J Nutr, 131, pp.1421~1426 (2001)
- Marles M.A., "New perspectives on proanthocyanidin biochemistry and molecular regulation", Phytochem, 64, pp.367~383 (2003)
- Mellway RD, "The wound-, pathogen-, and ultraviolet B-responsive MYB134 gene encodes an R2R3 MYB transcription factor that regulates proanthocyanidin synthesis in poplar", Plant Physiol., 150(2), pp.924~994 (2009)
- Oki T., "Polymeric procyanidins as radical -scavenging components in red-hulled rice", J Agric Food Chem, 50, pp.7524~7529 (2002)
- Revilla E., "Analysis of catechins and proanthocyanidins in grape seeds by HPLC with photodiode array detection", Chromatographia, 31, pp.465~468 (1991)
- Tanner G.J., "Proanthocyanidin biosynthesis in plants - Purification of legume leucoanthocyanidin reductase and molecular cloning of its cDNA", J Biol Chem, 278, pp.31647~31656 (2003)
- Winkel-Shirley B., "Flavonoid biosynthesis. A colorful model for genetics, biochemistry, cell biology, and biotechnology", Plant Physio., 126, pp.485~493 (2001)
- Xie D.Y., "Role of anthocyanidin reductase, encoded by BANYULS in plant flavonoid biosynthesis", Science, 299, pp.396~399 (2003)
- Xie D.Y., "Anthocyanidin reductases from Medicago truncatula and Arabidopsis thaliana", Arch Biochem Biophys, 422, pp.91~102 (2004)