종피색이 다른 벼 품종의 영과 쌀겨 추출물의 항산화 및 항암활성 변이
Variation of Antioxidant and Anticancer Activities of Hull and Bran Extracts in Different Colored Rices
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
In order to confirm the biological activities of rice bran and hull, total phenolic content, antioxidant and anticancer activities were examined in three rice varieties that have different seed coat colors such as brown, black, and purple. The antioxidant activity was measured by free radical scavenging assay including DPPH, ABTS+ and reducing power activity. The anticancer activity was evaluated by WST assay with human HepG2 cell lines. As the result, Huegjinju, black seed coat rice,showed higher of the total phenolics contents in both bran and hull, than purple Jeojinju and brown Ilpum. In the antioxidant activity, Heugjinju showed the highest among the bran extracts, but the activities of hull extracts were similar in three varieties. In the anticancer activity to HepG2 cell line, the hull extract was 20% higher than that of bran extract in the average of three varieties. The hull extracts of Ilpum and Jeokjinju showed similar anticancer activites about 92%, and that of Heugjinju showed the lowest activity of 30.4%. The anticancer activities of hull extracts in three varieties showed positive correlation with total phenolics contents, DPPH radical scavenging activities and reducing power activities. In conclusion, the rice bran and hull showed potent antioxidant and anticancer activities varied in three varieties that have different seed coat colors.
Abstract
In order to confirm the biological activities of rice bran and hull, total phenolic content, antioxidant and anticancer activities were examined in three rice varieties that have different seed coat colors such as brown, black, and purple. The antioxidant activity was measured by free radical scavenging assay including DPPH, ABTS+ and reducing power activity. The anticancer activity was evaluated by WST assay with human HepG2 cell lines. As the result, Huegjinju, black seed coat rice,showed higher of the total phenolics contents in both bran and hull, than purple Jeojinju and brown Ilpum. In the antioxidant activity, Heugjinju showed the highest among the bran extracts, but the activities of hull extracts were similar in three varieties. In the anticancer activity to HepG2 cell line, the hull extract was 20% higher than that of bran extract in the average of three varieties. The hull extracts of Ilpum and Jeokjinju showed similar anticancer activites about 92%, and that of Heugjinju showed the lowest activity of 30.4%. The anticancer activities of hull extracts in three varieties showed positive correlation with total phenolics contents, DPPH radical scavenging activities and reducing power activities. In conclusion, the rice bran and hull showed potent antioxidant and anticancer activities varied in three varieties that have different seed coat colors.
참고문헌 (28)
- Asamarai AM, "Wild rice hull antioxidants", J Agric Food Chem, 44, pp.126~130 (1996)
- Buxiang S, "Effects of co-administration of butylated hydroxytoluene, butylated hy droxy anisole and flavonoide on the activation of mutagens and drugmetabo- lizing enzyme in mice", Toxicology, 122, pp.61~72 (1997)
- 최원균, "국내산 식물의 항균활성 검색", 한국식품영양학회지, 15(4), pp.300~306 (2002), 한국식품영양학회
- Choi SW, "Isolation and identification of anthocyanin pigments in black rice", Foods Biotechnol, 3, pp.131~135 (1994)
- 이동진, "한국 재래종 벼 종실의 항산화 및 항암 활성", 한국국제농업개발학회지, 18(4), pp.264~269 (2006), 한국국제농업개발학회
- Halliwell, B, "Antioxidant in human health and disease", Annual Review in Nutrition, 16, pp.33~49 (1996)
- Hirose M, "Carcinogenicity of antioxidants BHA, caffeic acid, sesamol, 4-methoxyphenol and catechol at low doses, either alone or in", ~ (1998)
- Jeong JW, "Flavor components of citron juice as affected by the extraction method", Korean J. Food Sci Technol, 26, pp.709~712 (1994)
- 박지희, "왕겨 추출물의 항산화능에 대한 원적외선 처리의 효과", 한국식품영양과학회지, 34(1), pp.131~134 (2005), 한국식품영양과학회
- Kang MC, "Isolation and purification of r-Oryzanol in Rice Bran Using Accelerated Solvent Extractor" (2002), Seoul National University of Technology
- Ki-Min Woo, "Antioxidant Effect of Tocotrienol in Rice Bran", Korean. J. Crop Sci, 50, pp.4~7 (2005)
- Morrissey, P. A, "Dietary antioxidants in health and disease", Intermational Diary Journal, 8, pp.463~472 (1998)
- Namiki M, "Antioxidants", antimutagens in food, 1990, pp.29, 273~300
- Osawa T, "Novel natural antioxidants for utilization in food and biological systems, In Postharvest Biochemistry of Plant Food-Materials in the Tropics", pp.241~251 (1994), Japan Scientific Societies Press
- Osawa T, "Antioxidative defense systems in rice hull against damage caused by oxygen radecals", Agric Biol Chem, 49, pp.3085~3087 (1985)
- Oyaizu M, "Studies on products of browning reactions: anti antioxidant activity of products of browning reaction prepared from glucose amine", Japanes J. Nutrition, 44, pp.307~315 (1986)
- Pokorny J, "Natural antioxidant for food use", Trends food Sci Technol, 9, pp.223~227 (1991)
- Ramarathnam N, "Chimical studies on novel rice hull antioxydants Isolation, fractionation, and partial characterization", J Agric food chem, 36, pp.723~727 (1988)
- Ramarathnam N, "Studied on the relationship between antioxidative activity of rice hull and germination ability of rice seeds", J Sci food Agric, 37, pp.719~726 (1986)
- Re R, "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radical Biology and Medicine, 26, pp.1231~1237 (1999)
- -Jung Kim, "Cytotoxi and Antitumor Activity of Momilactone B from Rice Hulls", J Agric Food Chem, 55, pp.2702~2706 (2007)
- 공수현, "흑미의 도정분획 메탄올 추출물의 항산화 성분 및 항산화 효과", 한국식품영양과학회지, 37(7), pp.815~819 (2008), 한국식품영양과학회
- Su Noh Ryu, "Antioxidant Activity in Rice Cultivar , Wild Rice , and Barley", 한국작물학회지, 47(1), pp.54~61 (2002), 한국작물학회
- Tsuda T, "Antioxidative activity of the anthocyanin pigments cyandin 3-O-β-D-glucoside and cyaniding", J Agric Food Chem, 42, pp.2407~2411 (1994)
- Wu K, "Antioxidant properties of wild rice", J Agric food Chem, 42, pp.34~37 (1994)
- Xu Z, "Antioxidant activity of tocopherols, tocotrienols and r-oryzanol components from rice bran against cholesterol oxidation accelerated by 2,2'- azobis (2-methylpropionamidine) dihydrochloride", J Agric Food Chem, 49, pp.2077~2081 (2001)
- Byun-Woo Lee, "Response of Grain Yield and Milled-Rice Protein Content to Nitrogen Topdress Timing at Panicle Initiation Stage of Rice", 한국작물학회지, 51(1), pp.1~13 (2006), 한국작물학회
- 김용호, "근적외선분광분석기를 이용한 미강의 Tocopherol과 Tocotrienol 함량 분석", 한국작물학회지, 49(3), pp.211~215 (2004), 한국작물학회