한국 대표 밀 품종의 미숙배 채취 시기별 조직배양 효율
The Effects of Harvesting Time on the Efficiency of Tissue Culture Used Immature Embryos from Korean Wheat Cultivars
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초록
The regeneration rate of plantlets cultivated via tissue culture is an important factor for wheat transformation. Similar to othermonocotyledons, the most efficient tissue culture materials for wheat are immature embryos. However, stable year-round production of immatureembryos is not possible in the field where various stress factors co-exist. In this study, we investigated the generation and subsequent plantletincident rates of callus induced from immature embryos obtained from different sowing times in 2020 and compared these among wheatcultivars. We found that the rates of regeneration and plantlet incidence obtained using immature embryos of the Ariheuk cultivar were higherthan those of other domestic cultivars, and that the tissue culture efficiency was similar to that of Bobwhite, which has been establishedas a cultivar with excellent transformation efficiency. Furthermore, the Baekkang cultivar showed high tissue culture efficiency only whensown from early to mid-March, whereas Keumkang showed higher tissue cultivation efficiency only by sowing in mid- and late February. Among the five cultivars assessed in this study, Jopum showed the lowest tissue culture efficiency. It is anticipated that the findings of thisstudy will contribute to enhancing the transformation efficiency of domestic wheat varieties.
Abstract
The regeneration rate of plantlets cultivated via tissue culture is an important factor for wheat transformation. Similar to othermonocotyledons, the most efficient tissue culture materials for wheat are immature embryos. However, stable year-round production of immatureembryos is not possible in the field where various stress factors co-exist. In this study, we investigated the generation and subsequent plantletincident rates of callus induced from immature embryos obtained from different sowing times in 2020 and compared these among wheatcultivars. We found that the rates of regeneration and plantlet incidence obtained using immature embryos of the Ariheuk cultivar were higherthan those of other domestic cultivars, and that the tissue culture efficiency was similar to that of Bobwhite, which has been establishedas a cultivar with excellent transformation efficiency. Furthermore, the Baekkang cultivar showed high tissue culture efficiency only whensown from early to mid-March, whereas Keumkang showed higher tissue cultivation efficiency only by sowing in mid- and late February. Among the five cultivars assessed in this study, Jopum showed the lowest tissue culture efficiency. It is anticipated that the findings of thisstudy will contribute to enhancing the transformation efficiency of domestic wheat varieties.
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