Effects of Explant Source, Media and Growth Regulators on Shoot Regeneration of Soybean (Glycine max (L.) Merr.) in Vitro
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Abstract
This study was carried out to investigate the best explant source and combination of media and growth regulators for the regeneration of multiple shoots in soybean (Glycine max. L. Merr.) cv. Iksannamulkong. Multiple shoots could well be directly induced from both cotyledonary node and hypocotyl explants. However, more shoot were regenerated from cotyledons nodes than from hypocotyl explants. Among 5 different media tested, MSB medium, MS salts combined with B5 vitamins, was most effective for obtaining more regenerated shoots and higher ratio of explants (cotyledonary node) that give rise to regenerated shoots. All the three cytokinins, BA (2.0mg/l), Zeatin riboside (0.05mg/l) and thiazuron (TDZ, 2.0mg/l) were effective for obtaining enough number of regenerated shoot from cotyledonary nodes when added to MSB medium. The most effective cytokinin was zeatin riboside with an average number of 15.5 regenerated shoot per cotyledonary node and shoot regeneration was observed from 75% cotyledonary nodes plated. Therefore, for efficient shoot regeneration of soybean in vitro, it is recommended to plate cotyledonary node on MSB medium supplemented with 0.05mg/l zeatin riboside.
참고문헌 (16)
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