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SNP Discovery and Mapping of a Major Gene Rhg4 Conferring Resistance to Soybean Cyst Nematode

장현주|김문영|곽재균|장수연|반규정|이석하
한국육종학회 (The Korean Society of Breeding Science)|2004.06|한국육종학회지 (KOREAN JOURNAL OF BREEDING SCIENCE)|제36권 제2호|pp. 76-80

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참고문헌

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초록

Abstract

Two important loci, designated Rhg1 and Rhg4, are responsible for most resistance to soybean cyst nematode (SCN). The objective of this study was to survey single nucleotide polymorphism (SNP) and to map the SCN-resistant gene, Rhg4, on soybean genetic map. Direct sequencing with two primer sets Rhg4 gene revealed that three SNPs and seven indels were detected among six different soybean genotypes. The designed oligonucleotide complementary to the 5 region from the T(A)/C(G) SNP out of 10 SNPs detected was used in SNaPshot reactions, confirming polymorphism between PI 96188 and Jinju 1. Using a total of 90 recombinant inbred lines (RILs) derived from a cross between PI 96188?´?Jinju 1, the genetic population was mapped with 32 SSR markers, in which thirteen markers were placed into 5 linkage groups, covering 78.6 cM, and average distance between markers was 6.1 cM. Even though the genetic map was not completely saturated with SSR markers, linkage study revealed that Rhg4 was tightly linked to Satt187 on LG A2 with a distance of 6.8 cM. The location of Rhg4 on the genetic map is a good agreement with the previous report in the USDA map. From this study, it was concluded that sequence data availability of the responsible gene for the SCN resistance and sequence polymorphism between two parents map the gene successfully without phenotypic evaluation. In addition, SNP marker for SCN resistance map may be used in a marker-assisted selection for development of SCN-resistant soybean varieties in a breeding program.

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