Expression of resveratrol synthase gene and accumulation of resveratrol in transgenic potatoes (Solanum tuberosum L.)
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
A resveratrol synthase (RS) gene was isolated from peanut (Arachis hypogaea, L. cv. Jinpoong) plants. This gene was placed under the control of the cauliflower mosaic virus 35S promoter (CaMV35S) and introduced into two Korean varieties of potato (Solanum tuberosum L. cvs. Jasim and Jowon) plants by Agrobacterium-mediated gene transfer. Putative transformants were screened by PCR with primers designed from CaMV 35S promoter, NOS terminator and RS gene. Most of selected transgenic potato plants showed the amplification of expected fragments by PCR of genomic DNA with gene-specific primers, while they were absent in untransformed control plants. Expression of the resveratrol synthase gene was also examined by northern blot analysis. The transformants showed a band which was lacking in the control plant, confirming that the introduced gene is transcribed into mRNA in the transformants. The strength of the band, which reflected the level of mRNA expression, differed among the individual transformants. Among the transformants obtained, the highest trans-resveratrol content in the transgenic young leaves of purple-fleshed “Jashim” was 2.11 μgg-1 fresh weight and that in the microtubers in vitro of purple fleshed “Jashim” was 8.31 μgg-1 fresh weight. This amount of resveratrol may have a positive biological effect on human health.
Abstract
A resveratrol synthase (RS) gene was isolated from peanut (Arachis hypogaea, L. cv. Jinpoong) plants. This gene was placed under the control of the cauliflower mosaic virus 35S promoter (CaMV35S) and introduced into two Korean varieties of potato (Solanum tuberosum L. cvs. Jasim and Jowon) plants by Agrobacterium-mediated gene transfer. Putative transformants were screened by PCR with primers designed from CaMV 35S promoter, NOS terminator and RS gene. Most of selected transgenic potato plants showed the amplification of expected fragments by PCR of genomic DNA with gene-specific primers, while they were absent in untransformed control plants. Expression of the resveratrol synthase gene was also examined by northern blot analysis. The transformants showed a band which was lacking in the control plant, confirming that the introduced gene is transcribed into mRNA in the transformants. The strength of the band, which reflected the level of mRNA expression, differed among the individual transformants. Among the transformants obtained, the highest trans-resveratrol content in the transgenic young leaves of purple-fleshed “Jashim” was 2.11 μgg-1 fresh weight and that in the microtubers in vitro of purple fleshed “Jashim” was 8.31 μgg-1 fresh weight. This amount of resveratrol may have a positive biological effect on human health.
참고문헌 (22)
- An G, "Binary Ti vectors for plant transformation and promoter analysis", Methods Enzymol., 153, pp.292~305 (1987)
- An G, "Organization of the genes encoding chalcone synthase in Pisum sativum", Plant Mol. Biol., 21, pp.789~803 (1993)
- Baek SH, "Development of transgenic rice producing resveratrol", Korean J of Crop Science, 51, pp.141~ (2006)
- Bergmann H, "Activation of stress resistance in plants and consequences for product quality", Journal of Applied Botany, 73, pp.153~161 (1999)
- Bilodeau P, "A prolonged cold treatment-induced cytochrome P450 gene from Arabidopsis thaliana", Plant Cell Environ, 22, pp.791~800 (1999)
- Bokkenheuser VD, "Hydrolysis of dietary flavonoid glycosides by strains of intestinal Bacteroides from humans", Biochem J, 248, pp.953~956 (1987)
- Destefano-Beltran L, "Enhancing bacterial and fungal disease resistance in plants. Application to potato", pp.205~221 (1990), CABI. Wallingford
- Hain R, "Disease resistance results from foreign phytoalexin expression in a novel plant", Nature, 361, pp.153~156 (1993)
- Hart JH, "Role of phytostilbenes in decay and disease resistance", Annu Rev Phytopathol, 19, pp.437~458 (1981)
- Ingham JL, "3,5,4’-Trihydroxystilbene as a phytoalexin from groundnuts (Arachis hypogaea)", Phytochemistry, 15, pp.1791~1793 (1976)
- Jang M, "Cancer chemopreventive activity of resveratrol, a natural product derived from grapes", Scinece, 275, pp.218~220 (1997)
- Jeandet P, "HPLC analysis of grapevine phytoalexins coupling photodiode array detection and fluorometry", Anal Chem, 69, pp.5172~5177 (1999)
- 전태욱, "한국산 유색감자 색소의 이화학적 특성 및 생리 활성에 관한 연구", 한국식품과학회지, 37(2), pp.247~254 (2005), 한국식품과학회
- Kobayash S, "Kiwifruits (Actinidia deliciosa) transformed with a Vitis stilbene synthase gene produce piceid (resveratro-glucoside)", Plant Cell Reports, 19, pp.904~910 (2000)
- Langcake P, "The production of resveratrol by Vitis vinifera and other members of the Vitaceae as a response to infection of injury", Physiol Plant Pathol, 9, pp.77~86 (1976)
- Lawton MA, "Silencer region of a chalcone synthase promoter contains multiple sites for a factor SBF-1", closely related to GT-1 Plant Mol Biol, 16, pp.235~249 (1991)
- Leckband G, "Transformation and expression of a stilbene synthase gene of Vitis vinifera L", Theor Appl Genet, 96, pp.104~192 (1998)
- Murashige T, "A revised medium for rapid growth and bioassays with tobacco tissue cultures", Physiol Plant, 15, pp.473~497 (1962)
- Ruhmann S, "Piceid (resvertrol glucoside) synthesis in stilbene synthase transgenic apple fruit", J Agric food Chem, 54, pp.4633~4640 (2006)
- Stark-Lorenzen P, "Transfer of a grapevine stilbene synthase gene to rice (Oryza sativa L.)", Plant Cell Reports, 16, pp.668~673 (1997)
- Thomzik JE, "Gene transfer in plants", Pflanzenschutz Nachrichten Bayer, 48, pp.5~23 (1995)
- Wu JM, "Mechanism of cardioprotection by resveratrol, a phenolic antioxidant present in red wine", Int J Mol Med, 8, pp.3~17 (2001)