양파와 파간의 종간잡종 F1과 여교잡계통의 핵 DNA 함량
Variation of Nuclear DNA Content in Interspecific Allium cepa L.×A. fistulosum L. hybrids and Their Successive Backcross Lines
2
조회
0
다운로드
1
인용
0
참고문헌
초록
Abstract
Interspecific hybrid plants between Allium cepa L. (2n=2X=16) and A. fistulosum L. (2n=2X=16)and their backcross lines were developed by artificial pollination in order to introduce new desirable characters of A, cepa to A. fistulosum. The 2C nuclear DNA content has been estimated by flow cytometry in 5 Allium fistulosum inbreed lines, 2 interspecific hybrid lines of A. cepa×A. fistulosum and 34 their backcross lines BC1F1 to BC2F2, using propidium iodide (PI) as a fluorescence dye. Estimated 2C DNA values ranged from 22.2 pg to 23.7 pg in 5 A. fistulosum inbreed lines, 37.9 pg in F1 hybrid between A. cepa and A. fistulosum, 24.3 pg to 27.3 pg in 7 backcross lines in BC1F1, 21.9 pg to 24.4 pg in 9 BC1F2, 22.9 pg to 25.1 pg in 14 BC2F1, 22.6 pg to 23.4 pg in 4 BC2F2. This study showed mean 2C nuclear DNA content of F1 hybrid was higher than their backcross progeny lines, while it was lower than female parental line, A. cepa (2C DNA=33.2 pg). Mean 2C DNA content of backcross lines, BC1F1 to BC2F2 was not significantly different but their 2C DNA contents in the more progress generation from BC1F1 to BC2F2 were reduced.
참고문헌 (18)
- Baak EJ, "Hybridization and genome size evolution: Timing and magnitude of nuclear DNA content increases in Helianthus homoploid hybrid species", New phytologist., 167, pp.623~630 (2005)
- Bennett MD, "Nuclear DNA amounts in angiosperms", Phil Trans Roy Soc Lond, B., 274, pp.227~274 (1976)
- Dolezel J, "Plant DNA Flow Cytometry and Estimation of Nuclear Genome Size", Annals of Botany., 95, pp.99~110 (2005)
- Emsweller SL, "An interspecific hybrid in Allium", Hilgardia, 9, pp.265~273 (1935)
- Galbraith DW, "Analysis of nuclear DNA content and ploidy in higher plants" (1998), New York: John Wiley & Sons
- Kang H, "Studies on the interspecific hybridization between Allium fistulosum and A", cepa. J Kor Soc Hort Sci., 6, pp.57~72 (1967)
- Khrustaleva LI, "Cytogenetic studies in the bridge cross Allium cepa×(A. fistulosum×A. roylei)", Theor. Appl. Genet., 96, pp.8~14 (1998)
- Mangum PD, "Central cell nuclear-cytoplasmic incongruity: a mechanism for segregation distortion in advanced backcross and selfed generation of (Allium cepa L.×Allium fistulosum L.)×A. cepa interspecific hybrid derivatives", Cytogenet Genome Res., 109, pp.400~407 (2005)
- Pfosser M, "Evaluation of sensitivity of flow cytometry in detecting aneuploidy in wheat using disomic and ditelosomic wheat -rye addition lines", Cytometry., 21, pp.387~393 (1995)
- Ricroch A, "DNA base composition of Allium genomes with different chromosome numbers", Gene, 205, pp.255~260 (1997)
- Ulloa G, "Chromosome characteristics and behavior differences in Allium fistulosum L., A. cepa L., their F1hybrid, and selected backcross progeny", Theor Appl Genet., 89, pp.567~571 (1994)
- Ulloa G, "Evidence for nuclear cytoplasmic incompatibility between Allium fistulosum and A", cepa. Theor Appl Genet., 90, pp.746~754 (1995)
- Umehara M, "Interspecific hybrids between Allium fistulosum and Allium schoenoprasum reveal carotene-rich phenotype", Euphytica., 148, pp.295~301 (2006)
- Van der Valk P, "Independent segregation of two isozyme makers and inter-plant difference in nuclear DNA content in the interspecific backcross (Allium fistulosum × cepa L.) ×A. cepa L", Euphytica, 55, pp.151~156 (1991)
- Van der Meer QP, "Improving the onion crop (Allium cepa L.) by transfer of characters from Allium fistulosum L", Biul Warzywinczy., 22, pp.87~91 (1978)
- Van't Hof J, "relationships between mitotic cycle duration, S period duration and the average rate of DNA synthesis in the root meristem cells of several plants", Exp Cell Res., 39, pp.8~58 (1965)
- Wang ML, "Construction of a chromosome-enriched Hpall library from flow-sorted wheat chromosomes", Nucleic Acids Res., 20, pp.1897~1901
- Yamasita K, "Seed production test of CMS line of Japanese bunching onion (Allium fistulosum L.) possing the cytoplasm of wild species, A. galanthum Kar. et Kir", Euphytica, 136, pp.327~331 (2004)