인삼 유전자원 장기보존을 위한 종자 초저온보존 조건 구명
Response of Pre and Post Treatments for Cryopreservation of Korean Ginseng Seeds on Recovering Viability
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We investigated the effect of pre and post-cryopreservation treatment on the dehisced Ginseng (Panax ginseng C.A. Meyer) seeds germination rate. All seeds covered with endocarp and 96.3% of them were opened, initial moisture content (IMC)and germination rate were 55.9% and 84.0%, respectively. According to the pre and post-cryopreservation methods, highest seedsgermination rate (GR) was observed in non-precooled seeds which recovered at 40°C, in which MC was 8-12% and desiccatedat 15℃. However, by prolonged drying the seeds GR comparatively decreased under MC of below 2.2%, reaching below rate4% regardless of pre and post- cryo treatments, whereas the seeds dried at 25℃ airflow cabinet saved significantly high rate. The effects of desiccation and of cryopreservation on the survival and developmental pattern of roots and stems showed that theearliest appearance of the root in control seeds was detected after 10 days of bedding in GA medium, and the stem started todevelop later on the third week of germination test. And, on the 30th day of the investigation stem growth rate significantlyincreased and showed equivalent data with root rate. Almost the same pattern showed the seeds after cryopreservation, but itsignificantly delayed in the development compared to control, where term of the investigation continued till 40 day than control30 day. Additionally, non-precooling seeds in both desiccation and recovery methods were showed high root and stem growthcompared to precooled treatments.
Abstract
We investigated the effect of pre and post-cryopreservation treatment on the dehisced Ginseng (Panax ginseng C.A. Meyer) seeds germination rate. All seeds covered with endocarp and 96.3% of them were opened, initial moisture content (IMC)and germination rate were 55.9% and 84.0%, respectively. According to the pre and post-cryopreservation methods, highest seedsgermination rate (GR) was observed in non-precooled seeds which recovered at 40°C, in which MC was 8-12% and desiccatedat 15℃. However, by prolonged drying the seeds GR comparatively decreased under MC of below 2.2%, reaching below rate4% regardless of pre and post- cryo treatments, whereas the seeds dried at 25℃ airflow cabinet saved significantly high rate. The effects of desiccation and of cryopreservation on the survival and developmental pattern of roots and stems showed that theearliest appearance of the root in control seeds was detected after 10 days of bedding in GA medium, and the stem started todevelop later on the third week of germination test. And, on the 30th day of the investigation stem growth rate significantlyincreased and showed equivalent data with root rate. Almost the same pattern showed the seeds after cryopreservation, but itsignificantly delayed in the development compared to control, where term of the investigation continued till 40 day than control30 day. Additionally, non-precooling seeds in both desiccation and recovery methods were showed high root and stem growthcompared to precooled treatments.
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