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Insulin Enhances Nitric Oxide Production in Trabecular Meshwork Cells via De Novo Pathway for Tetrahydrobiopterin Synthesis

김재우
대한안과학회 (The Korean Ophthalmological Society)|2007.03|Korean Journal of Ophthalmology|제21권 제1호|pp. 39-44

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참고문헌

주제분류안과학

초록

Abstract

Purpose: To investigate the effect of insulin on the production of nitric oxide (NO) in the trabecular meshwork (TM) cells and the enzymatic synthetic pathway of tetrahydrobiopterin (BH4) synthesis. Methods: Primarily cultured human TM cells were exposed to 1, 10, and 100 &micro;g/ml of insulin and 0, 1, 10, 100 and 1000 nM dexamethasone for 3 days. To evaluate the enzymatic pathway of BH4 synthesis, 10 &micro;M dexamethasone, 5 mM diaminopyrimidinone, 100 &micro;M ascorbic acid, 100 &micro;M sepiapterin, or 10 &micro;M methotrexate were also co-administered respectively. Cellular survival and NO production were measured with MTT and Griess assay. Results: Insulin enhanced NO production in a dose-dependent manner significantly (p<0.05) without affecting cell viability, whereas dexamethasone inhibited NO production. With co-exposure of insulin, diaminopyrimidinone and sepiapterin inhibited insulin-induced NO production. Ascorbic acid increased NO production independent of insulin and methotrexate did not affect to the action of insulin in NO production. Conclusions: Insulin increases NO production in TM cells via de novo synthetic pathway for BH4 synthesis. Insulin could be involved in the regulation of trabecular outflow by enhancing NO production in TM cells.

참고문헌 (50)

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관련 논문AI추천

Effect of Advanced Glycation End Products on Oxidative Stress and Senescence of Trabecular Meshwork Cells김재우, 박철호대한안과학회 · Korean Journal of Ophthalmology2012 · 제26권 제2호Effect of Nitric Oxide on the Expression of Matrix Metalloproteinase and Its Association with Migration of Cultured Trabecular Meshwork Cells김재우대한안과학회 · Korean Journal of Ophthalmology2016 · 제30권 제1호Comparative Study of the Effects of Trabecular Meshwork Outflow Drugs on the Permeability and Nitric Oxide Production in Trabecular Meshwork Cells김재우대한안과학회 · Korean Journal of Ophthalmology2017 · 제31권 제5호Effect and Mechanism of Phosphodiesterase Inhibitors on Trabecular Outflow김재우, 이소형, 이종빈대한안과학회 · Korean Journal of Ophthalmology2019 · 제33권 제5호섬유주세포에서 니트로글리세린에 의한 일산화질소의 생성경로에 관한 연구김재우, 윤숙현대한안과학회 · 대한안과학회지2013 · 제54권 제9호Maintenance of cellular tetrahydrobiopterin homeostasis김혜림, 박영식생화학분자생물학회 · BMB Reports2010 · 제43권 제9호

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