산소유발망막병증 쥐 모델에서 커큐민의 효과
Effect of Curcumin in a Mouse Model of Oxygen-Induced Retinopathy
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목적: Hypoxia-inducible factor-1을 억제하는 것으로 알려진 커큐민을 산소유발망막병증 쥐 모델에 투여하여 커큐민의 망막 신생 혈관에 미치는 영향을 알아보고자 하였다. 대상과 방법: C57BL/6 mouse 를 생후 7일(P7)부터 5일 동안은 75%의 고산소 환경에, 이후 5일간 정상 산소 환경에 노출시켜 산소유발망막병증 모델을 제작하였다. 전신 투여군은 P12부터 5일간 하루 한 번 커큐민을 복강내 주사하고, 유리체강내 주사군은 P13에 커큐민을 1회 유리체강내 주사하였다. P17에 안구를 적출해 망막 신생혈관 발달 정도를 커큐민을 투여하지 않은 각 대조군과 비교하였다. 결과: 복강내 및 유리체강내 커큐민 투여군 모두에서 망막 flat mount상 신생혈관의 발생 정도는 대조군에 비해 유의한 차이를 보이지않았다. 망막 단면의 신생혈관 정량 결과에서도 커큐민 투여군과 대조군간의 유의한 차이가 나타나지 않았다. 결론: 산소유발망막병증 동물 모델에서 복강내 및 유리체강내 커큐민 투여는 망막 신생혈관을 감소시키지 못했다. 커큐민을 망막 신생혈관 억제제로 개발하기 위해서는 제형 개선 등 추가 연구가 필요하다.
Abstract
Purpose: To investigate the effect of curcumin, known to inhibit hypoxia-inducible factor-1, on retinal neovascularization in a mouse model of oxygen-induced retinopathy (OIR). Methods: OIR was induced by exposing C57BL/6 mice on postnatal day 7 (P7) to 75% hyperoxia for 5 days, followed by 5days in a room with normal oxygen level. Curcumin was administered intraperitoneally once a day for 5 days from P12 or intravitreally once on P13. Mice retinas on P17 were analyzed for retinal neovascularization, which was compared between curcumin-treated and control mice. Results: After intraperitoneal and intravitreal administration of curcumin, qualitative assessment of retinal neovascularization of flat-mounted retina showed no significant difference compared to control retinas. Quantitative assessment of retinal neovascularization also showed no significant difference between curcumin-treated and control mice. Conclusions: Both intraperitoneal and intravitreal administration of curcumin did not reduce retinal neovascularization in an OIR mouse model. Further investigation including development of new formulations is required for the use of curcumin as an anti-angiogenic agent for retinal neovascularization.
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