The effect of baicalin in a mouse model of retinopathy of prematurity
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Baicalin is a flavonoid derived from the dried root of Scutellaria baicalensis. In this study, oxygen-induced retinopathy was used to characterize the anti-angiogenic properties of baicalin in mice. Pups were exposed to a hyperbaric oxygen environment to induce retinal angiogenesis and were subjected to intraperitoneal injection of baicalin. Avascular area, neovascular tufts, and neovascular lumens were quantified from digital images. Compared to the vehicle, baicalin clearly reduced the central avascular zone and the number of neovascular tufts and lumens. High-dose baicalin (10 mg/kg) significantly reduced the expression of matrix metalloproteinase-2 (MMP-2), MMP-9, angiotensin II, and vascular endothelial growth factor (VEGF). These results show that baicalin is a powerful antiangiogenic compound that attenuates new vessel formation in the retina after systemic administration, and is a candidate substance for therapeutic inhibition of retinal angiogenesis.
Abstract
Baicalin is a flavonoid derived from the dried root of Scutellaria baicalensis. In this study, oxygen-induced retinopathy was used to characterize the anti-angiogenic properties of baicalin in mice. Pups were exposed to a hyperbaric oxygen environment to induce retinal angiogenesis and were subjected to intraperitoneal injection of baicalin. Avascular area, neovascular tufts, and neovascular lumens were quantified from digital images. Compared to the vehicle, baicalin clearly reduced the central avascular zone and the number of neovascular tufts and lumens. High-dose baicalin (10 mg/kg) significantly reduced the expression of matrix metalloproteinase-2 (MMP-2), MMP-9, angiotensin II, and vascular endothelial growth factor (VEGF). These results show that baicalin is a powerful antiangiogenic compound that attenuates new vessel formation in the retina after systemic administration, and is a candidate substance for therapeutic inhibition of retinal angiogenesis.
참고문헌 (19)
- Chow LC, "Can changes in clinical practice decrease the incidence of severe retinopathy of prematurity in very low birth weight infants?", Pediatrics, 111, pp.339~345 (2001)
- Shalev B, "Randomized comparison of diode laser photocoagulation versus cryotherapy for threshold retinopathy of prematurity: seven-year outcome", Am J Ophthalmol, 132, pp.76~80 (2001)
- Autrata R, "Cryotherapy and photocoagulation in the treatment of retinopathy of prematurity", Cesk Slov Oftalmol, 58, pp.30~35 (2002)
- McDonald HR, "Visual loss following panretinal photocoagulation for proliferative diabetic retinopathy", Ophthalmology, 92, pp.388~393 (1985)
- Lerner BC, "Transient myopia and accommodative paresis following retinal cryotherapy and panretinal photocoagulation", Am J Ophthalmol, 97, pp.704~708 (1984)
- Liu JJ, "Baicalein and baicalin are potent inhibitors of angiogenesis: Inhibition of endothelial cell proliferation, migration and differentiation", Int J Cancer, 106, pp.559~565 (2003)
- Miocinovic R, "In vivo and in vitro effect of baicalein on human prostate cancer cells", Int J Oncol, 26, pp.241~246 (2005)
- Zhang K, "Baicalin increases VEGF expression and angiogenesis by activating the ERR{alpha}", PGC-1{alpha} pathway, 2010, pp.89, 426~435
- Yang SJ, "Baicalin Attenuates Laser-Induced Choroidal Neovascularization", Curr Eye Res, 39, pp.745~751 (2014)
- Deng YF, "Inhibitory activities of baicalin against renin and angiotensin-converting enzyme", Pharm Biol, 50, pp.401~406 (2012)
- Sarlos S, "Retinal angiogenesis is mediated by an interaction between the angiotensin type 2 receptor, VEGF, and angiopoietin", Am J Pathol, 163, pp.879~887 (2003)
- Shieh DE, "Antioxidant and free radical scavenging effects of baicalein, baicalin and wogonin", Anticancer Res, 20, pp.2861~2865 (2000)
- Huang WH, "Antioxidative and anti-inflammatory activities of polyhydroxyflavonoids of Scutellaria baicalensis GEORGI", Biosci Biotechnol Biochem, 70, pp.2371~2380 (2006)
- Smith LE, "Oxygeninduced retinopathy in the mouse", Invest Ophthalmol Vis Sci, 35, pp.101~111 (1994)
- 조형, "Sulodexide inhibits retinal neovascularization in a mouse model of oxygen-induced retinopathy", BMB Reports, 47(11), pp.637~642 (2014), 생화학분자생물학회
- Nanni C, "Retro-orbital injection is an effective route for radiopharmaceutical administration in mice during small-animal PET studies", Nucl Med Commun, 28, pp.547~553 (2007)
- Connor KM, "Quantification of oxygen-induced retinopathy in the mouse: a model of vessel loss, vessel regrowth and pathological angiogenesis", Nat Protoc, pp.1565~1573 (2009)
- Stahl A, "Computeraided quantification of retinal neovascularization", Angiogenesis, 12, pp.297~301 (2009)
- 임지홍, "Zinc finger and BTB domain-containing protein 3 is essential for the growth of cancer cells", BMB Reports, 47(7), pp.405~410 (2014), 생화학분자생물학회