Bone Marrow-derived Mesenchymal Stem Cells Affect Immunologic Profiling of Interleukin-17-secreting Cells in a Chemical Burn Mouse Model
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Purpose: This study investigated interleukin (IL)-17-secreting cell involvement in sterile inflammation, and evaluatedthe effect of mesenchymal stem cells (MSCs) on IL-17-secreting cell immunologic profiling. Methods: Twenty mice were sacrificed at time points of 6 hours, 1 day, 1 week, and 3 weeks (each group, n =5) after the cornea was chemically injured with 0.5N NaOH; IL-17 changes in the cornea were evaluated usingenzyme-linked immunosorbent assay. Further, IL-17 secreting cells were assessed in the cervical lymph nodesby a flow cytometer. Rat MSCs were applied intraperitoneally in a burn model (n = 10), IL-17-secreting T helper17 (Th17) cell and non-Th17 cell changes were checked using a flow cytometer in both cornea and cervicallymph nodes at 1week, and compared with those in the positive control (n = 10). Results: IL-17 was highest in the cornea at 1 week, while, in the cervical lymph nodes, IL-17-secreting cellsshowed early increase at 6 hours, and maintained the increase through 1 day to 1 week, and levels returnedto the basal level at 3 weeks. Specifically, the non-Th17 cells secreted IL-17 earlier than the Th17 cells. Whenthe MSCs were applied, IL-17 secretion was reduced in CD3(+)CD4(-)CD8(-), CD3(+)CD4(+)CD8(-), and CD3(+)CD4(-)CD8(+) cells of the cervical lymph nodes by 53.7%, 43.8%, and 50.8%, respectively. However, in thecornea, IL-17 secretion of CD3(+)CD4(-)CD8(-) cells was completely blocked. Conclusions: The results indicated that both IL-17-secreting non-Th17 and Th17 cells were involved in the chemicalburn model, and MSCs appeared to mainly modulate non-Th17 cells and also partially suppress the Th17 cells.
Abstract
Purpose: This study investigated interleukin (IL)-17-secreting cell involvement in sterile inflammation, and evaluatedthe effect of mesenchymal stem cells (MSCs) on IL-17-secreting cell immunologic profiling. Methods: Twenty mice were sacrificed at time points of 6 hours, 1 day, 1 week, and 3 weeks (each group, n =5) after the cornea was chemically injured with 0.5N NaOH; IL-17 changes in the cornea were evaluated usingenzyme-linked immunosorbent assay. Further, IL-17 secreting cells were assessed in the cervical lymph nodesby a flow cytometer. Rat MSCs were applied intraperitoneally in a burn model (n = 10), IL-17-secreting T helper17 (Th17) cell and non-Th17 cell changes were checked using a flow cytometer in both cornea and cervicallymph nodes at 1week, and compared with those in the positive control (n = 10). Results: IL-17 was highest in the cornea at 1 week, while, in the cervical lymph nodes, IL-17-secreting cellsshowed early increase at 6 hours, and maintained the increase through 1 day to 1 week, and levels returnedto the basal level at 3 weeks. Specifically, the non-Th17 cells secreted IL-17 earlier than the Th17 cells. Whenthe MSCs were applied, IL-17 secretion was reduced in CD3(+)CD4(-)CD8(-), CD3(+)CD4(+)CD8(-), and CD3(+)CD4(-)CD8(+) cells of the cervical lymph nodes by 53.7%, 43.8%, and 50.8%, respectively. However, in thecornea, IL-17 secretion of CD3(+)CD4(-)CD8(-) cells was completely blocked. Conclusions: The results indicated that both IL-17-secreting non-Th17 and Th17 cells were involved in the chemicalburn model, and MSCs appeared to mainly modulate non-Th17 cells and also partially suppress the Th17 cells.
참고문헌 (33)
- Bassi EJ, "Immune regulatory properties of multipotent mesenchymal stromal cells: where do we stand?", World J Stem Cells, 3, pp.1~8 (2011)
- Bassi EJ, "Exploring the role of soluble factors associated with immune regulatory properties of mesenchymal stem cells", Stem Cell Rev, 8, pp.329~342 (2012)
- kop DJ, "Mesenchymal stem", stromal cells (MSCs): role as guardians of inflammation, 2012, pp.20, 14~20
- Shi Y, "Mesenchymal stem cells: a new strategy for immunosuppression and tissue repair", Cell Res, 20, pp.510~518 (2010)
- Chen SJ, "Current status of the immunomodulation and immunomediated therapeutic strategies for multiple sclerosis", Clin Dev Immunol, 2012, pp.970789~ (2012)
- Raychaudhuri SP, "Role of IL-17 in psoriasis and psoriatic arthritis", Clin Rev Allergy Immunol, 44, pp.183~193 (2013)
- Marwaha AK, "TH17 cells in autoimmunity and immunodeficiency: protective or pathogenic?", Front Immunol, 3, pp.129~ (2012)
- Maddur MS, "Th17 cells: biology, pathogenesis of autoimmune and inflammatory diseases, and therapeutic strategies", Am J Pathol, 181, pp.8~18 (2012)
- Hirota K, "Regulation and function of innate and adaptive interleukin-17-producing cells", EMBO Rep, 13, pp.113~120 (2012)
- Fossiez F, "Interleukin-17", Int Rev Immunol, 16, pp.541~551 (1998)
- Duffy MM, "Mesenchymal stem cell inhibition of T-helper 17 cell- differentiation is triggered by cell-cell contact and mediated by prostaglandin E2 via the EP4 receptor", Eur J Immunol, 41, pp.2840~2851 (2011)
- Duffy MM, "Mesenchymal stem cell effects on T-cell effector pathways", Stem Cell Res Ther, 2, pp.34~ (2011)
- Ghannam S, "Mesenchymal stem cells inhibit human Th17 cell differentiation and function and induce a T regulatory cell phenotype", J Immunol, 185, pp.302~312 (2010)
- Ohshima M, "Systemic transplantation of allogenic fetal membrane-derived mesenchymal stem cells suppresses Th1 and Th17 T cell responses in experimental autoimmune myocarditis", J Mol Cell Cardiol, 53, pp.420~428 (2012)
- Qu X, "Mesenchymal stem cells inhibit Th17 cell differentiation by IL-10 secretion", Exp Hematol, 40, pp.761~770 (2012)
- Prigione I, "Reciprocal interactions between human mesenchymal stem cells and gammadelta T cells or invariant natural killer T cells", Stem Cells, 27, pp.693~702 (2009)
- Sasaki JR, "Burn induces a Th-17inflammatory response at the injury site", Burns, 37, pp.646~651 (2011)
- Ye J, "Mesenchymal stem cell transplantation in a rabbit corneal alkali burn model: engraftment and involvement in wound healing", Eye (Lond), 20, pp.482~490 (2006)
- Ye J, "Bone marrow-derived progenitor cells promote corneal wound healing following alkali injury", Graefes Arch Clin Exp Ophthalmol, 246, pp.217~222 (2008)
- Yao L, "Role of mesenchymal stem cells on cornea wound healing induced by acute alkali burn", PLoS One, 7, pp.e30842~ (2012)
- Oh JY, "The anti-inflammatory and anti-angiogenic role of mesenchymal stem cells in corneal wound healing following chemical injury", Stem Cells, 26, pp.1047~1055 (2008)
- Ma Y, "Reconstruction of chemically burned rat corneal surface by bone marrow-derived human mesenchymal stem cells", Stem Cells, 24, pp.315~321 (2006)
- Church D, "Burn wound infections", Clin Microbiol Rev, 19, pp.403~434 (2006)
- Oh JY, "Identification of the HSPB4", TLR2, EMBO Mol M ed, pp.2012, 4[DOI]
- Tazetdinova NR, "Changes in the T-lymphocyte subpopulations in patients with severe eye burns", Oftalmol Zh, (6), pp.335~338 (1990)
- Zhao M, "Immunologic experimental studies on the alkali burn of cornea in rats", Zhonghua Yan Ke Za Zhi, 36, pp.40~42 (2000)
- Darlington PJ, "Reciprocal Th1and Th17 regulation by mesenchymal stem cells: Implication for multiple sclerosis", Ann Neurol, 68, pp.540~545 (2010)
- Rubio D, "Spontaneous human adult stem cell transformation", Cancer Res, 65, pp.3035~3039 (2005)
- Wang Y, "O utgrowth of a transformed cell population derived from normal human BM mesenchymal stem cell culture", Cytotherapy, 7, pp.509~519 (2005)
- Miura M, "Accumulated chromosomal instability in murine bone marrow mesenchymal stem cells leads to malignant transformation", Stem Cells, 24, pp.1095~1103 (2006)
- Zhou YF, "Spontaneous transformation of cultured mouse bone marrow-derived stromal cells", Cancer Res, 66, pp.10849~10854 (2006)
- Aguilar S, "Murine but not human mesenchymal stem cells generate osteosarcoma-like lesions in the lung", Stem Cells, 25, pp.1586~1594 (2007)
- Tolar J, "Sarcoma derived from cultured mesenchymal stem cells", Stem Cells, 25, pp.371~379 (2007)