Analysis of Changes in Retinal Photoreceptors Using Optical Coherence Tomography in a Feline Model of Iodoacetic Acid-induced Retinal Degeneration
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Purpose: We investigated structural changes in the retina by using optical coherence tomography (OCT) in afeline model of retinal degeneration using iodoacetic acid (IAA). Methods: We examined 22 eyes of 11 felines over 2 years of age. The felines had fasted for 12 hours and wereintravenously injected with IAA 20 mg/kg of body weight. OCT (Spectralis OCT) was performed at the pointwhere the ends of the retinal vessels collected in the lateral direction from the optic nerve head and areacentralis. Similarly, OCT was performed four times at 1-week intervals following injections, at which point thefelines were sacrificed and histologic examinations were performed. Using OCT, the thickness of each layer ofthe retina was measured. Results: The average body weight of the three male and eight female felines investigated in this study was1.61 ± 0.19 kg. The mean total retinal thickness of the felines before injection was 221.32 ± 9.82 μm, with asignificant decrease in the retinal thickness at 2, 3, and 4 weeks following injections of 186.41 ± 35.42, 174.56± 31.94, and 175.35 ± 33.84 μm, respectively (p = 0.028, 0.027, and 0.027, respectively). The thickness of theouter nuclear layer was 57.49 ± 8.03 μm before injection and 29.26 ± 17.87, 25.62 ± 13.88, and 31.60 ± 18.38μm at 2, 3, and 4 weeks, respectively, after injection (p = 0.028, 0.028, 0.046, respectively). Conclusions: In a feline model of retinal degeneration using IAA, the total retinal thickness and the thickness ofthe outer nuclear layer were shown to decrease significantly on OCT.
Abstract
Purpose: We investigated structural changes in the retina by using optical coherence tomography (OCT) in afeline model of retinal degeneration using iodoacetic acid (IAA). Methods: We examined 22 eyes of 11 felines over 2 years of age. The felines had fasted for 12 hours and wereintravenously injected with IAA 20 mg/kg of body weight. OCT (Spectralis OCT) was performed at the pointwhere the ends of the retinal vessels collected in the lateral direction from the optic nerve head and areacentralis. Similarly, OCT was performed four times at 1-week intervals following injections, at which point thefelines were sacrificed and histologic examinations were performed. Using OCT, the thickness of each layer ofthe retina was measured. Results: The average body weight of the three male and eight female felines investigated in this study was1.61 ± 0.19 kg. The mean total retinal thickness of the felines before injection was 221.32 ± 9.82 μm, with asignificant decrease in the retinal thickness at 2, 3, and 4 weeks following injections of 186.41 ± 35.42, 174.56± 31.94, and 175.35 ± 33.84 μm, respectively (p = 0.028, 0.027, and 0.027, respectively). The thickness of theouter nuclear layer was 57.49 ± 8.03 μm before injection and 29.26 ± 17.87, 25.62 ± 13.88, and 31.60 ± 18.38μm at 2, 3, and 4 weeks, respectively, after injection (p = 0.028, 0.028, 0.046, respectively). Conclusions: In a feline model of retinal degeneration using IAA, the total retinal thickness and the thickness ofthe outer nuclear layer were shown to decrease significantly on OCT.
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