본문 바로가기
KERD
논문KCI 등재

Changes of Neuropathic Pain in nNOS Knock-out Mice

이영호|김민정|이건수|양준영|송수성|목우균
대한체질인류학회|2005.12|해부·생물인류학 (Anatomy & Biological Anthropology)|제18권 제4호|pp. 303-312|DOI 10.11637/aba.2005.18.4.303

1

조회

0

다운로드

0

인용

0

참고문헌

키워드

Nitric oxidenNOSnitric oxide신경병증성 통증Neuropathic painAllodynia이질통
주제분류해부학

초록

신경손상 후 척수나 후근신경절에서 NO 생성 변화가 이질통(allodynia)에 관여하는 것으로 알려져 있다. 반면 nNOS의 발현이 이질통의 유발과 유지에 관여하지 않는다는 보고도 있다. 본 연구에서는 감각신경계통의 nNOS의 발현이 신경병증성 통증에서의 역할을 규명하고자 하였다. 정상조군 및 nNOS 결핍 생쥐에서 말초신경 손상 후 von Frey 및 아세톤 테스트를 시행하였으며, 이들 쥐에 nNOS 억제제를 투여하여 통증의 변화를 확인하였다. nNOS 결핍 생쥐에서는 말초신경 손상 후 정상대조군 생쥐보다 물리적 이질통이 약간 감소하였다. 정상대조군에서는 nNOS 억제제인 L-NAME 투여에 의해 물리적 이질통이 약간 감소하였다. 반면에, gabapentin은 정상대조 군 및 nNOS 결핍 생쥐 모두에서 신경병증성 통증(물리적 및 냉 이질통)을 현저히 감소시켰다. 이상의 결과는 생쥐의 신경병증성 모델에서 감각신경계의 nNOS의 발현이 물리적 이질통의 유발과 유지에 일부 관여하고 있음을 보여준다.

Abstract

Changes in nitric oxide production in spinal cord or dorsal root ganglion have been known to contribute to allodynia after nerve injury. However, regulation of nNOS expression was also reported not to be responsible for the development and/or maintenance of neuropathic allodynia. The aim of this study was to elucidate role of nNOS expression in the sensory nervous system in neuropathic pain. Von Frey and acetone tests were performed in a model of peripheral neuropathy, ligation of 5th lumbar and 6th lumbar spinal nerves, in wild type and nNOS (-/-) mice. The effect of nNOS inhibitor was evaluated in neuropathic pain behavior in the mice. Mechanical allodynia was slightly reduced in nNOS (-/-) mice compared with wild type mice after peripheral neuropathy. nNOS inhibitor, L-NAME, reduced minimally mechanical allodynia, not cold allodynia, but gabapentin reduced remarkably neuropathic pain behavior (mechanical and cold allodynia) in both wild type and nNOS (-/-) mice. These results suggested that nNOS expression in the sensory nervous system may be partially associated with development and/or maintenance of mechanical allodynia in a mouse model of peripheral neuropathy.

참고문헌 (36)

  1. "Modality-specific hyper-responsivityof regenerated cat cutaneous nociceptors", pp.897~906 (1999)
  2. "Systemic lidocaine in pain due to peripheral nerve injuryand predictors of response", 62, pp.218~225 (2004)
  3. "warm and cold allodynia in a rat model ofperipheral neuropathy", pp.341~344 (2004)
  4. "A peripheral mononeuropathy in ratthat produces disorders of pain sensation like those seen inman", 133, pp.87~107 (1988)
  5. "Gabapentin in the treatment ofneuropathic pain", 18, pp.5~11 (2004)
  6. "Nitric oxide synthase protein and mRNA arediscretely localized in neuronal populations of the mammalianCNS together with NADPH diaphorase", 7, pp.615~624 (1991)
  7. "Spinal pharmacology of tactileallodynia in diabetic rats", 122, pp.1478~1482 (1997)
  8. "Efficacy of spinalNMDA receptor antagonism in formalin hyperalgesia andnerve injury evoked allodynia in the rat", 280, pp.829~838 (1997)
  9. "Neuropathic pain inrats is associated with altered nitric oxide synthase activityin neural tissue", 138, pp.14~20 (1996)
  10. "Neuropathicpain is associated with alterations of nitric oxide synthaseimmunoreactivity and catalytic activity in dorsal root gangliaand spinal dorsal horn", 58, pp.161~171 (2002)
  11. "Nitirc oxide synthase and neuronal NADPH-diaphoraseare identical in brain and peripheral tissues", 88, pp.7797~7801 (1991)
  12. "Nitric oxide synthase- role as atrasmitter mediator in the brain and endocrine system", 47, pp.219~227 (1996)
  13. "NADPH-diaphorasereaction and c-JUN co-expression in rat dorsalroot ganglion neurons following sciatic nerve transection", pp.169~173 (1993), Long-lasting increase ofnitric oxide synthase immunoreactivity
  14. "Comparativeactivity of the anti-convulsants oxcarbazepine lamotrigine and gabapentin in a model ofneuropathic pain in the rat and guinea-pig", pp.355~362 (2003)
  15. "Gabapentin reverses mechanical allodynia induced bysciatic nerve ischemia and formalin-induced nociceptionin mice", 182, pp.427~434 (2003)
  16. "Role of nitric oxide in a spinal ligation model of neuropathicpain", 43 (2002)
  17. "Difficulties in stratifying neuropathic pain bymechanisms", 7, pp.353~357 (2003)
  18. "An experimental model for peripheralneuropathy produced by segmental spinal nerve ligation inthe rat", 50, pp.355~363 (1992)
  19. "The NMDAreceptorantagonist CPP abolishes neurogenic ‘wind-uppain’ after intrathecal administration in humans", 51, pp.249~253 (1992)
  20. "Gabapentin is a first line drug for the treatment of neuropathicpain in spinal cord injury", 29, pp.743~751 (2004)
  21. "Local nitric oxide synthase activityin a model of neuropathic pain", 10, pp.1846~1855 (1998)
  22. "Neuronal nitric oxide synthase mRNA upregulationin rat sensory neurons after spinal nerve ligation lack of a role in allodynia development", chaplan sr, pp.9201~9208 (1999)
  23. "Intrathecaltreatment with dextrorphan or ketamine potently reducespain-related behaviors in a rat model of peripheral mononeuropathy", 605, pp.164~168 (1993)
  24. "Central hyperexcitabilitytriggered by noxious inputs", pp.602~610 (1993)
  25. "Nitric oxidemediates the thermal hyperalgesia produced in a model ofneuropathic pain in the rat", 50, pp.7~10 (1992)
  26. "Cloning of induciblenitric oxide synthase in rat vascular smooth muscle cells", 191, pp.89~94 (1993)
  27. "Newer antiepileptic drugs:possible uses inthe treatment of neuropathic pain and migrane", pp.2506~2538 (2003)
  28. "Systemic ketamine attenuatesnociceptive behaviors in a rat model of peripheralneuropathy", 715, pp.51~62 (1996)
  29. "The nitric oxide-cyclic GMP pathway isrequired for nociceptive signalling at specific loci withinthe somatosensory pathway", 73, pp.649~655 (1996)
  30. "Therole of injury discharge in the induction of neuropathicpain behavior in rats", 46, pp.327~336 (1991)
  31. "Molecular cloningand expression of a cDNA encoding endothelial cell nitricoxide synthase", pp.15274~15276 (1992)
  32. "Increased nitric oxide synthase immunoreactivity inrat dorsal root ganglia in a neuropathic pain model", 169, pp.81~84 (1994)
  33. "on injured nerve attenuates neuropathy-induced thermal hyperalgesia in rats", pp.124~210 126 (1996)
  34. "Marked increase in nitric oxide synthase mRNA in ratdorsal root ganglia after peripheral axotomy", pp.11617~11621 (1992)
  35. "Role of nitric oxide in thedevelopment of thermal hyperesthesia induced by sciaticnerve constriction injury in the rat", 82, pp.1266~1273 (1995)
  36. "Nitric oxide mediates behavioralsigns of neuropathic pain in an experimental ratmodel", 9, pp.367~372 (1998)

이 간행물의 다른 논문 (7건)

부분계측값을 이용한 넙다리뼈 최대길이 산출한기환, 김이석, 박대균, 김덕임, 한승호, 이우영, 곽대순, 이제훈pp. 247-254넓적다리 앞가쪽유리피판술을 위한 가쪽넓은근의뿌리동맥과 피부분포영역신채수, 한승호, 남용석, 백두진pp. 255-262한국인 청소년 운동선수군과 일반인에서 ACE 유전자의삽입/결실 다형성의 분포에 관한 연구장민희, 이강오, 백두진, 이재구, 강병용pp. 263-270흰쥐 뇌에 분포하는 항원제공세포의 연령에 따른 변화정호석, 김형동, 유기수pp. 272-282Micro -computed tomography를 이용한 골다공증의새로운 형태학적 연구기법우대곤, 고창용, 김한성, 이법이pp. 283-290
원문 보기