흰쥐 심장근육에 분포하는 MHC class II 양성반응 수지상세포의 연령에 따른 변화
Age-related Changes of MHC Class II Positive Dendritic Cells in Cardiac Muscle of Rat
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초록
심장에서는 면역반응에 중요한 역할을 하는 major histocompatibility complex (MHC) class II 양성 면역반 응 수지상세포가 분포한다고 알려져 있으나 연령에 따른 세포수의 차이와 형태 변화에 대해서는 아직 밝혀져 있 지 않다. 이에 연구자들은 나이가 다른 실험동물을 비교하여 연령에 따른 변화를 밝히고자 이 연구를 시행하였다. 실험동물로는 1개월, 12개월, 24개월의 수컷 Sprague-Dawley계 흰쥐 15마리를 사용하였다. 흰쥐에 3.5% chloral hydrate (1 mL/100 g)를 복강내주사하여 마취시킨 후 심장을 절취하여 동결절편을 제작하였다. 동결절편에 일차항 체인 mouse anti-rat MHC class II antibody를 이용하여 면역염색을 시킨 후 심장근육에 분포하는 양성 면역반응 수 지상세포들을 광학현미경으로 검경과 계수하였다. 흰쥐 심장근육에 분포하는 수지상세포들의 수는 생후 1개월 때는 단위면적(0.2mm2) 당 평균 1.4개, 생후 12개 월 때는 평균 2.8개, 그리고 생후 24개월 때는 평균 4.6개로 나이가 많아짐에 따라 세포수는 증가하였다. 세포의 형태도 나이가 많아짐에 따라 세포질돌기가 많아지고 길어졌으며, 일부 세포들은 군집을 이루는 경우가 많았다. 그러므로 연령이 증가함에 따라 수지상세포의 수와 형태의 변화는 면역기능에 영향을 미칠 것으로 생각된다.
Abstract
Cardiac dendritic cells are considered to play an important role in the immunoresponse of the heart. However, It is unknown that changes of shapes and numbers of these cells in the heart. The aim of this study is to reveal age-related changes of MHC class II positive dendritic cells in cardiac muscle of rat. Male Sprague-Dawley rats (1 month, 12 months, and 24 months old) were used in this study. Animals were deeply anesthetized with 3.5% chloral hydrate (1 mL/100 g) and hearts removed. Immunostaining was done according to standard methods used routinely. In brief, tissue sections were incubated with primary antibodies generated in mouse anti-rat MHC class II antibody for single immunostains. Tissue sections were observed by using light microscope and dendritic cells were counted. Average numbers of MHC class II-positive dendritic cells were 1.4 cells per unit area (0.2 mm2) at 1 month old rat, 2.8 cells at 12 months old rat, and 4.6 cells at 24 months old rat, and then numbers of dendritic cells were increased according to ages. According as age increases, cytoplasmic processes of MHC class II-immunoreactive dendritic cells became longer and more complex and aggregated together. It’s suggested that age-related changes of MHC class II positive dendritic cells in the cardiac muscle would be related to immunity.
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