GRβ과발현이 스테로이드에 의한 IL-8 합성 및 분비에 미치는 영향
Effect of GRβ Over-expression on Steroid-induced Suppression of IL-8 Synthesis and Secretion
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Abstract
Background: There are few reports about the effect of GRβ on glucocorticoid-induced suppression of inflammatory cytokines, and GRβ as a dominant negative inhibitor of GRα signaling is still uncertain. Objective: We investigated the functional role of GRβ expression in glucocorticoid-induced suppression of IL-8 synthesis and secretion. Method: Expression of mRNA and secretion of IL-8 were measured by RT-PCR and ELISA, respectively, in the A549 lung epithelial cell lines. Suppression of IL-8 productioninduced by glucocorticoid was compared with or without pCMV GRβ over-expression. Result: Expression of mRNA and secretion of IL-8 were not affected with overexpression of GRβ. GRβ had some suppressive effects on IL-8 production, but was not significant. Conclusion: This study suggests that GRβ do not act as a dominant negative inhibitor of GRα in the steroid-induced suppression of IL-8 in the airway epithelial cell, and that GRβ expression may not be the major cause of steroid resistance.
참고문헌 (34)
- "multifactorial states of clinical importance", ki(t), pp.609~13
- "Cellular mechanisms contributing to inadequate response to glucocorticoid therapy", pp.33~9
- "Glucocorticoid resistance in asthma"
- "clinical characteristics of steroid-insensitive asthma", pp.594~601
- "Update on glucocorticoid action and resistance", pp.3~22
- "Association of glucocorticoid insensitivity with increased expression of glucocorticoid receptor β", pp.1567~74
- "Increased glucocorticoid receptor beta in airway cells of glucocorticoid-insensitive asthma", pp.1600~4
- "Glucocorticoid resistance in asthma is associated with elevated in vivo expression of the glucocorticoid receptor beta-isoform", stay(v dz), pp.943~50, Sousa AR
- "High constitutive glucocorticoid receptor β in human neutrophils enables them to reduce their spontaneous rate of cell death in response to corticosteroids", pp.585~94
- "The dominant degative activity of the human glucocorticoid receptor β isoform Specificity and mechanisms of action", pp.27857~66
- "Increased glucocorticoid receptor β expression converts mouse hybridoma cells to a corticosteroid-insensitive phenotype", pp.361~7
- "Molecular origins for the dominant negative function of human glucocorticoid receptor beta", pp.4319~30
- "Glucocorticoid receptor α and β in glucocorticoid dependent asthma", pp.7~13
- "Glucocorticoid receptor isoform α and β in in vitro cytokine- induced glucocorticoid insensitivity", pp.420~25
- "Evidence that the β-isoform of the human glucocorticoid receptor dose not act as a physiologically significant repressor", pp.26659~64
- "The dominant negative activity of the human glucocorticoid receptor β isoform Speceficity and mechanisms of action", pp.27857~66
- "Effect of GRβ expression on steroid-induced transcriptional activity in airway epithelial cells", pp.810~7
- "Essential involvement of interleukin-8", pp.559~8 64
- "Role of leukotriene B4 in bronchial hyperresponsiveness induced by interleukin 8", pp.306~11
- "Increase of chemokine levels in sputum precedes exacerbation of acute asthma attacks", pp.313~6
- "GM-CSF IL-1R TNF-αR and HLA-DR in nasal epithelial cells in allergic rhinitis", pp.~8
- "Expression of the potent inflammatory cytokines granulocyte-macrophage-colony- stimulating factor and interleukin-6 and interleukin-8 in bronchial epithelial cells of patients with asthma"
- "Proinflammaroty cytokines regulate human glucocorticoid receptor gene expression and lead to the accumulation of the dominant negative β isoform a mechanism for the generation of glucocorticoid resistance", pp.6865~70
- "Molecular mechanisms of corticosteroids in allergic diseases", pp.928~36
- "The human glucocorticoid receptor β isoform Expressionand putative function", pp.9550~9
- "Expression and subcellular distribution of the β-isoform of the human glucocorticoid receptor", pp.5028~38
- "Overexpression of the human glucocorticoid receptor α and β isoforms inhibits AP-1 and NF-κB activates hormone independently", pp.309~18
- "Defective glucocorticoid receptor nuclear translocation and altered histone acetylation patterns in glucocorticoid-resistant patients", pp.1100~8
- "Antagonism of glucocorticoid receptor transcriptional activation by the c-Jun N-terminal kinase", pp.2050~5
- "role in steroid-insensitive asthma", pp.649~57
- "Glucocorticoid receptor recruitment of histone deacetylase 2 inhibits interleukin-1β-induced histone H4 acetylation on lysine 8 and 12", pp.6891~903
- "Repression of IL-5 transcription by glucocorticoid receptor targets GATA3 signalling and involves histone deacetylase recruitment", in press
- "Corticosteroid resstance in chronic pulmonary disease inactivation of histone deacetylase", pp.731~3
- "Corticosteroid-insensitive asthma molecular mechanisms", pp.347~55
