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Interleukin-8 (IL-8) 분비에 미치는 neuropeptides의 영향에 관한 연구

The effect of neuropeptides on secretion of Interleukin-8 (IL-8)

박상혁|최경규|박상진|김경준
대한치과보존학회 (Korean Academy Of Conservative Dentistry)|2006.05|Restorative Dentistry and Endodontics|제31권 제3호|pp. 153-160

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참고문헌

주제분류치의학

초록

Interleukin-8(IL-8) 분비에 미치는 neuropeptides의 영향에 관한 연구

Abstract

We investigated the secretion of Interleukin-8(IL-8) from ginviva and periodontal ligament stimulated with Substance P(SP) and Calcitonin Gene-related Peptide(CGRP). Gingiva(GF), periodontal ligament(PDLF) and pulp(PF) tissues were collected from extracted intact 3rd molars.Cultured cells were stimulated with different concentrations of SP for 4 hrs, and stimulated with SP, CGRP and Tumor Necrosis Factor-α(TNF-α) for 8 hrs. Then RNase Protection Assay was carried out. ELISA was performed using supernatants of stimulated cells for quantitative analysis of IL-8. Results were assessed using student t-test with significance of P<0.05.According to this study, the results were as follows:1. IL-8 mRNA was detected in all type of cells studied(PF, GF, and PDLF).2. IL-8 mRNA expression was not increased after stimulating 4 hrs with SP(10-5M) and SP(10-8M) compared with Mock stimulation in all type of cells studied.3. IL-8 mRNA expression was not increased after stimulating 8 hrs with SP(10-4M) and CGRP(10-6M) compared with Mock stimulation in all type of cells studied.4. TNF-α(2 ng/ml) increased the expression of IL-8 mRNA in all kind of cells studied.5. The secretion of IL-8 from GF was increased 8 hrs after the stimulation with CGRP(10-6M)(P<0.05).6. The secretion of IL-8 from PDLF was increased 8 hrs after the stimulation with SP(10-4M)(P<0.05).Calcitonin Gene-related Peptide(CGRP) increased Interleukin-8(IL-8) which plays an important role in chemotaxis of neutrophil in Calcitonin Gene-related Peptide(CGRP) gingival tissue, whereas Substance P increased the secretion of IL-8 from periodontal ligament.

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