척수손상 후 숨뇌와 척수회색질기둥 앞뿔에 분포하는 calbindin D-28K와 parvalbumin면역반응세포에 관한 형태학적 연구
Morphological studies on the calbindin D-28K and parvalbumin immunoreactive neurons in the medulla oblongata and ventral horn of the spinal cord gray matter after spinal cord injury in rats
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척수를 손상시키지 않은 정상 군과 완전히 손상시킨 군 그리고 각각 오른쪽과 왼쪽을 반씩 손상시킨 후 5, 10일동안 사육한 흰쥐의 숨뇌와 각 척수 분절의 회색질기둥 앞뿔에서 칼슘결합단백질인 calbindin D-28K (CB) 와 parvalbumin (PV)에 단세포군 항체를 이용하여 면역조직화학적 방법으로 염색하여 CB-IR세포와 PV-IR세포 의 분포양상과 세포의 크기 및 형태에 대하여 관찰하여 다음과 같은 결과를 얻었다. 1. 숨뇌의 그물핵에서 CB-IR세포와 PV-IR세포를 관찰할 수 있었다. 2. CB-IR세포와 PV-IR세포는 척수 각 분절의 회색질기둥 앞뿔의 앞가쪽핵, 뒤안쪽핵, 뒤가쪽핵, 뒤안쪽핵, 중앙핵 등에서 관찰할 수 있었다. 특히 허리분절에서 강한 양성 반응을 나타내었다. 3. 척수 손상 후 5일간 사육한 실험군의 경우 완전히 손상 받은 가슴분절 아래부위에서는 CB-IR세포와 PV-IR세 포는 드물게 관찰되었다. 4. 척수 손상 후 10일간 사육한 실험군에서 왼쪽 손상부위와 오른쪽 손상부위에서 CB-IR세포와 PV-IR세포의 수는 거의 비슷하였다. 5. CB-IR세포와 PV-IR세포는 방추형, 타원형, 원형, 삼각형이었으며 크기는 40~50 µm 정도였고, 모양은 홑극, 두 극, 뭇극, 수평형이었다. 이상의 실험 결과를 요약하면 척수 손상 후 5, 10일간 사육한 실험군에서 CB-IR세포와 PV-IR세포는 각 척수분 절의 회색질기둥 앞뿔의 VII~X층에서 가장 많이 관찰 할 수 있었다.
Abstract
This study was examined and compared the immunocytochemical distribution of the two calcium-binding proteins calbindin D-28K and parvalbumin immunreactive neurons in the medulla oblongata and spinal cord after transection of spinal cord in rats. In this experiment, calbindin D-28K immnunoreactive neurons were mainly found in many pyramidal cells distributed medulla oblongata and spina1 cord of rats. Parvalbumin immunoreactive cells were demonstrated in all lamina of the gray matter of the spinal cord. These immunoreactive cells had the most high density in the severa1 nuclei of the ventra1 horn of the all segments of the spina1 cord. Calbindin D-28K neuropil labeling was strongly noted in spina1 all segments of the spinal cord. In contrast parva1bumin immunoreactive, little differences were found in distribution, size and morphology of calbindin D-28K cell body or neuropil staining in the spinal cord. The number of parvalbumin immunoreactive cells were more than twice in the medulla oblongata and spinal cord compared to the calbindin D-28K immunoreactive cells. Calbindin D-28K and parvalbumin-immmoreactive somata were round, ova1, spind1e and polygona1 in shape, and the immunoreactive neurons were unipolar, bipolar, multipolar and horizontal in shape. The diameters of the somata of the two immunoreactive neurons were 40~50 µm, respectively. Also dendrites of two immunoreactive neurons were densely arrayed in network. These results suggest that CB-IR and PV-IR most high density in the of the VII~X layers in the ventra1 horn of the all segments of the spina1 cord.
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