Melatonin이 MDPC-23 세포의 분화에 미치는 영향
Effects of Melatonin on MDPC-23 Cells Differentiation
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초록
Melatonin은 솔방울샘(pineal gland)에서 분비되는 주요 호르몬으로 신체의 여러 생리학적 및 병태생리학 적 조절에 관여한다. 최근의 연구들에 따르면 melatonin은 뼈모세포 분화와 바탕질의 석회화를 촉진시켜 골성장 조절에 있어 중요한 역할을 하는 것으로 알려졌다. 본 연구에서는 상아질모세포 전구세포인 MDPC-23 세포를 이용하여 melatonin이 상아질모세포 분화를 촉진시 키는 지를 확인하였다. MDPC-23 세포에 분화 배양액과 melatonin을 첨가하여 분화를 유도시킨 후, RT-PCR을 이 용하여 dentin sialophosphoprotein (DSPP), osteocalcin (OC), alkaline phosphatase (ALP)와 nuclear factor I-C (NFI-C) 의 발현 변화를 분석하여 다음과 같은 결과들을 얻었다. 석회화 결절의 형성은 melatonin의 농도가 증가할수록 촉진되었으며, 특히 분화 15일째에 대조군에 비해 석회 화 결절의 형성이 강하게 관찰되었다. DSPP와 OC의 발현은 melatonin의 농도증가에 따라 증가된 반면에, ALP의 발현은 melatonin의 농도에 따라 변화가 없었다. NFI-C의 발현은 melatonin의 농도증가에 따라 배양 초기에 대조 군에 비해 크게 감소하였다. 위의 결과들을 종합하면 melatonin은 상아질모세포 전구세포인 MDPC-23 세포의 분화와 석회화를 촉진시키며, 상아질 형성에 중요한 역할을 하는 것으로 생각된다.
Abstract
Melatonin is the major hormone released from the pineal gland and regulates a variety of physiological and pathophysiological processes. According to the recent studies the melatonin plays an important role in regulation of bone growth. The purpose of this study was to determine whether melatonin promotes the cell differentiation and nodules formation in MDPC-23 pre-odontoblast cell line. MDPC-23 cells were cultured for up to 15 days in growth media containing differentiation medium with melatonin or without melatonin. Cultures were stained with Alizarin-S. The expression of the mRNAs for DSPP, OC, ALP and NFI-C were analyzed by RT-PCR. The results were as follows. Cultures containing melatonin at day 15 showed extensive mineralization as compared with control cultures. Melatonin increased the expression of DSPP and OC mRNAs in MDPC-23 cells in a concentration- dependent manner. However, melatonin did not changed ALP expression. Melatonin markedly decreased mRNA expression of NFI-C in early stage cultures as compared with control cultures. These results demonstrated that melatonin is capable of promoting MDPC-23 cells differentiation and mineralization and suggested that melatonin may play an important role in dentin formation.
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