노화 흰쥐 뇌에서 Kv1.2의 분포에 관한 면역조직화학적 연구
Immunohistochemical Study on the Distribution of Kv1.2 in Aged Rat Brain
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본 연구에서는 흰쥐 뇌에서 Kv1.2 면역염색성의 노화에 따른 변화를 규명하였다. 성숙 흰쥐와 노화 흰쥐를 사용하였고 면역조직화학법을 이용하여 염색한 후 영상분석을 실시하였다. 노화 흰쥐의 대뇌겉질에서, Kv1.2 면역염색성은 피라미드 세포의 가지돌기와 세포체에서 주로 관찰되었다. 띠겉 질, 변연계아래 겉질 및 조롱박겉질 등의 대뇌겉질의 여러 부위에서 면역염색성을 나타내는 신경세포들의 수가 증가했다. 노화 흰쥐의 고유해마의 CA1 부위에서는 몇몇 중간 크기의 신경세포의 세포체와 돌기에서 중간정도의 Kv1.2 면역염색성을 보였다. 노화 흰쥐의 편도핵에서는 Kv1.2 면역염색성을 나타내는 신경세포의 세포체에서 염 색정도가 증가한 반면, 염색된 신경세포의 수는 대조군과 비교하여 별다른 차이를 보이지 않았다. 노화에 따른 염 색성의 증가가 가장 뚜렷한 부위는 뇌줄기였다. Kv1.2 면역염색성을 나타내는 신경세포의 수와 염색강도는 얼굴 신경핵, 솔기핵, 다리뇌 및 숨뇌 그물체에서 증가하였다. 본 연구에서 노화에 따른 신경퇴행성 변화의 복합적인 기전에 대해서는 정확히 밝히지 못했지만, Kv1.2 채널 발현의 노화에 따른 변화에 대한 본 연구는 노화 및 노화와 관련된 신경퇴행성질환의 병태생리의 이해에 도움을 줄 것이라 사료된다.
Abstract
In the present study, we demonstrated age-related changes in Kv1.2 immunoreactivity in the rat brain for the first time. Twelve adult (4~6 month old) and 15 aged (20~29 month old) Sprague-Dawley rats were examined in this study. Immunohistochemistry was performed in accordance with the free-floating method, and densitometric measurement using a NIH image program (Scion Image) determined the staining density. In the cerebral cortex of aged rats, there was a significant increase in the number of Kv1.2-immunoreactive neurons in the cingulate cortex, infralimbic cortex and piriform cortex, compare to adult rats. In the hippocampal CA1-3 regions, moderate Kv1.2 immunoreactivity was found in the cell bodies and processes of some medium to large-sized neurons in aged rats. The intensity was increased in the cell bodies of Kv1.2-positive neurons in the amygdala of aged rats, whereas the number of immunoreactive neurons was not significantly increased. It was noteworthy that age-related changes in Kv1.2-immunoreactive neurons were prominent in the facial nuclei, raphe magnus nuclei, and pontine and medullary reticular formation. Although the present study has not addressed multiple mechanisms contributing to neuronal degeneration during aging, the first demonstration of age-related changes in Kv1.2 immnuoreactivity may offer a comprehensive understanding of the pathophysiology of aging and age-related neurodegenerative diseases such as Alzheimer’s disease.
참고문헌 (16)
- Ajmani RS, "Hemodynamic changes during aging associated with cerebral blood flow and impaired cognitive function", 21, pp.257~269 (2000)
- Chung YH, "Immunohistochemical study on the distribution of voltage-gated K+ channels in rat brain following transient focal ischemia", 308, pp.157~160 (2001)
- Conforti L, "Selective inhibition of a slow-inactivating voltage-dependent K+ channel in rat PC12 cells by hypoxia", 502, pp.293~305 (1997)
- Conforti L, "O2-sensitive K+ channels: role of the Kv1.2 -subunit in mediating the hypoxic response", 524, pp.783~793 (2000)
- Duprat F, "Susceptibility of cloned K+ channels to reactive oxygen species", 92, pp.11796~11800 (1995)
- Gao TM, "Changes in membrane properties of CA1 pyramidal neurons after transient forebrain ischemia in vivo", 90, pp.771~780 (1999)
- Mitani A, "Delayedneuronal death is induced without post-ischemic hyperexcitability continuous multiple-unit recording from ischemicCA1 neurons", pp.819~823 (1991)
- Koller H, "Time course of inwardly rectifying K+ current reduction in glial cells surrounding ischemic brain lesions", 872, pp.194~198 (2000)
- Leblond J, "Hypoxic changes in hippocampalneurons", pp.1~14 (1989)
- Lee J-M, "Brain tissue responses to ischemia", 106, pp.723~731 (2000)
- Matsuura H, "Effects of oxidant stress on steadystatebackground currents in isolated ventricular myocytes" (1991)
- Petito CK, "Delayed neuronal recovery andneuronal death in rat hippocampus following severe cerebralischemia possible relationship to abnormalities inneuronal processes"
- Brierley JB, "Temporal profile of neuronaldamage in a model of transient forebrain ischemia", pp.491~498 (1982)
- Stocker M, "Regulation of fast inactivation of clonedmammalian IKA channels by cysteine oxidation", pp.711~714 (1991), Heinemann SH
- Urban L, "Postischemicsynaptic physiology in area CA1 of the gerbilhippocampus studied in vitro", pp.3966~3975 (1989)
- Zola Morgan S, "Enduring memory impairment in monkeys afterischemic damage to the hippocampus", pp.2582~2596 (1992)