Tryptophan Negatively Regulates IgE-mediated Mast Cell Activation
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Mast cells are major immune cells in allergy to secrete allergic mediators by a degranulation process and make and secrete inflammatory lipids and cytokines in response to antigen stimulation. An amino acid tryptophan regulates immune functions. Tryptophan ameliorates inflammatory colitis in which mast cells are engaged. However, its effects on mast cells remain to be solved. We investigated the effect of tryptophan on IgE-mediated allergic responses in the mast cells and mice. IgEmediated passive cutaneous anaphylaxis (PCA) in mice were examined. Also IgE-mediated mast cell activation responses such as degranulation of stored granules and secretion of inflammatory lipid LTB4 and cytokines (TNF-α and IL-4) were measured. Intraperitoneal administration of tryptophan suppressed PCA in mice. Also, in the cellular level tryptophan inhibited IgE-mediated mast cell activation such as IgE-mediated degranulation and the production of LTB4. Also, it inhibited production of inflammatory cytokines TNF-α and IL-4. In summary, tryptophan suppressed IgE-mediated allergic activation in vivo and in vitro. Tryptophan supplementation is beneficial for IgE-mediated allergy.
Abstract
Mast cells are major immune cells in allergy to secrete allergic mediators by a degranulation process and make and secrete inflammatory lipids and cytokines in response to antigen stimulation. An amino acid tryptophan regulates immune functions. Tryptophan ameliorates inflammatory colitis in which mast cells are engaged. However, its effects on mast cells remain to be solved. We investigated the effect of tryptophan on IgE-mediated allergic responses in the mast cells and mice. IgEmediated passive cutaneous anaphylaxis (PCA) in mice were examined. Also IgE-mediated mast cell activation responses such as degranulation of stored granules and secretion of inflammatory lipid LTB4 and cytokines (TNF-α and IL-4) were measured. Intraperitoneal administration of tryptophan suppressed PCA in mice. Also, in the cellular level tryptophan inhibited IgE-mediated mast cell activation such as IgE-mediated degranulation and the production of LTB4. Also, it inhibited production of inflammatory cytokines TNF-α and IL-4. In summary, tryptophan suppressed IgE-mediated allergic activation in vivo and in vitro. Tryptophan supplementation is beneficial for IgE-mediated allergy.
참고문헌 (18)
- Bischoff SC, "Role of mast cells in allergic and non-allergic immune responses: comparison of human and murine data", Nat Rev Immunol, 7, pp.93~104 (2007)
- Gregory GD, "Mast cells in allergy and autoimmunity:implications for adaptive immunity", Methods Mol Biol, 315, pp.35~50 (2006)
- Gilfillan AM, "Integrated signalling pathways for mast-cell activation", Nat Rev Immunol, 6, pp.218~230 (2006)
- Kalesnikoff J, "New developments in mast cell biology", Nat Immunol, 9, pp.1215~1223 (2008)
- Moffett JR, "Tryptophan and the immune response", Immunol Cell Biol, 81, pp.247~265 (2003)
- Kim CJ, "l-Tryptophan exhibits therapeutic function in a porcine model of dextran sodium sulfate (DSS)-induced colitis", J Nutr Biochem, 21, pp.468~475 (2010)
- Hamilton MJ, "Essential role for mast cell tryptase in acute experimental colitis", Proc Natl Acad Sci U S A, 108, pp.290~295 (2011)
- Oellerich MF, "FOXOs and sirtuins in vascular growth, maintenance, and aging", Circ Res, 110, pp.1238~1251 (2012)
- Bekier E, "The inhibitory effect of nicotinamide on asthma-like symptoms and eosinophilia in guinea pigs, anaphylactic mast cell degranulation in mice, and histamine release from rat isolated peritoneal mast cells by compound 48-80", Int Arch Allergy Appl Immunol, 47, pp.737~748 (1974)
- Silwal P, "Adenine suppresses IgE-mediated mast cell activation", Mol Immunol, 65, pp.242~249 (2015)
- Park SK, "Src-like adaptor protein (SLAP) is upregulated in antigen-stimulated mast cells and acts as a negative regulator", Mol Immunol, 46, pp.2133~2139 (2009)
- Abbas AK, "Hypersensitivity disorders. In Cellular and molecular immunology", pp.407~444 (2012), Elsevier
- Bischoff SC, "Role of mast cells in allergic and non-allergic immune responses: comparison of human and murine data", Nat Rev Immunol, 7, pp.93~104 (2007)
- Boyce JA, "Mast cells and eicosanoid mediators: a system of reciprocal paracrine and autocrine regulation", Immunol Rev, 217, pp.168~185 (2007)
- Theoharides TC, "Mast cells and inflammation", Biochim Biophys Acta, 1822, pp.21~33 (2012)
- Rivera J, "Molecular regulation of mast cell activation", J Allergy Clin Immunol, 117, pp.1214~1225 (2006)
- Kawasaki H, "A tryptophan metabolite, kynurenine, promotes mast cell activation through aryl hydrocarbon receptor", Allergy, 69, pp.445~452 (2014)
- Uozumi N, "Role of cytosolic phospholipase A2 in allergic response and parturition", Nature, 390, pp.618~622 (1997)