칼륨 섭취에 따른 콩팥 사이세포들의 변화
Response of Renal Intercalated Cells to Dietary Potassium Intake
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현대 인류는 고대시대 인류보다 칼륨 (K+) 섭취가 현저히 부족하다. 콩팥의 집합관은 칼륨 항상성을 조절하는 곳으로, 다양한 세포들로 구성되어 있다. 본 연구의 목적은 칼륨 섭취에 따른 집합관 사이세포들 (intercalated cells)의 반응을 관찰하는 것이다. C57BL/6 생쥐를 대조군 (1% 칼륨), 저칼륨식이 (<0.01%), 또는 고칼륨식이 (5%) 군으로 나누고, 사료를 1주간 섭취시켰다. 콩팥 조직을 2% paraformaldehyde-lysine-periodate로 관류고정한 후, H&E염색 및 면역조직화학법을 시행하였다. 혈중 칼륨 농도는 대조군 (4.3±0.41 mmol/L)에 비해 저칼륨식이 섭취군(2.6±0.53 mmol/L)에서 유의하게 감소하였으며, 고칼륨식이군 (5.0±0.38 mmol/L)에서는 증가하였다. 저칼륨식이로 인한 형태변화는 집합관에서 뚜렷하게 나타났으며, 세포들이 증식하고 비대되었다. 이미지 분석 결과, A형 사이세포들은 저칼륨식이군에서 크기가 약 1.6배 증가하였으며, 고칼륨식이군에서는 감소하였다. 이와 반대로, B형과 NANB형 사이세포들은 저칼륨식이군에서 크기가 감소하고, 고칼륨식이군에서는 증가하는 양상을 보였다. 이상의 결과로 집합관 사이세포들의 형태는 칼륨 섭취에 따라 크게 달라질 수 있음을 증명하였다. 칼륨 불균형으로 인한 콩팥의 구조 변화는 현대 인류의 심혈관 및 콩팥질환을 이해하는 데 도움을 줄 수 있을 것으로 생각된다.
Abstract
While ancient humans consumed enough potassium (K+), modern humans have insufficient K+ intake. The renal collecting duct plays an important role in regulating K+ homeostasis. There are at least three different types of cells in the collecting duct. The purpose of this study is to examine the effects of dietary K+ intake on collecting duct intercalated cells. C57BL/6 mice had free access to a control (1%), low (<0.01%), or high (5%) K+ diet for 1 week. Kidney tissue were processed for light microscopic immunohistochemistry and image analysis. Excessive dietary K+ intake or deficiency significantly changed blood K+ concentrations in mice. The size of type A intercalated cells increased by about 1.6 times in the low K+ group and decreased in the high K+ group. Conversely, the size of type B intercalated cells decreased in the low K+ group and increased in the high K+ group. The response of NANB cells to dietary K+ intake was generally like type B cells. These results demonstrated that renal intercalated cells vary greatly with dietary K+ intake. The structural changes in response to K+ imbalance may be closely related to cardiovascular and renal disorders in modern humans.
참고문헌 (31)
- Youn JH, "Recent advances in understanding integrative control of potassium homeostasis", Annu Rev Physiol, 71, pp.381~401 (2009)
- He FJ, "Beneficial effects of potassium on human health", Physiol Plant, 133, pp.725~735 (2008)
- Palmer BF, "Achieving the Benefits of a High-Potassium, Paleolithic Diet, Without the Toxicity", Mayo Clin Proc, 91, pp.496~508 (2016)
- Lee HS, "Sodium and potassium intake patterns and trends in South Korea", J Hum Hypertens, 27, pp.298~303 (2013)
- Perez V, "Sodium-to-potassium ratio and blood pressure, hypertension, and related factors", Adv Nutr, 5, pp.712~741 (2014)
- McDonough AA, "Cardiovascular benefits associated with higher dietary K(+) vs. lower dietary Na(+): evidence from population and mechanistic studies", Am J Physiol Endocrinol Metab, 312, pp.E348~E356 (2017)
- Kamel KS, "Renal potassium physiology : integration of the renal response to dietary potassium depletion", Kidney Int, 93, pp.41~53 (2018)
- Adrogué HJ, "Sodium surfeit and potassium deficit : keys to the pathogenesis of hypertension", J Am Soc Hypertens, 8, pp.203~213 (2014)
- Brunner HR, "The influence of potassium administration and of potassium deprivation on plasma renin in normal and hypertensive subjects", J Clin Invest, 49, pp.2128~2138 (1970)
- Krishna GG, "Increased blood pressure during potassium depletion in normotensive men", N Engl J Med, 320, pp.1177~1182 (1989)
- Ferraro PM, "Dietary Protein and Potassium, Diet-Dependent Net Acid Load, and Risk of Incident Kidney Stones", Clin J Am Soc Nephrol, 11, pp.1834~1844 (2016)
- Dreier R, "Effect of Increased Potassium Intake on Adrenal Cortical and Cardiovascular Responses to Angiotensin II : A Randomized Crossover Study", J Am Heart Assoc, 10, pp.e018716~ (2021)
- Wang WH, "Regulation of potassium(K)handling in the renal collecting duct", Pflugers Arch, 458, pp.157~168 (2009)
- Feraille E, "Coordinated Control of ENaC and Na+, K+-ATPase in Renal Collecting Duct", J Am Soc Nephrol, 27, pp.2554~2563 (2016)
- Aronson PS, "Effects of pH on potassium : new explanations for old observations", J Am Soc Nephrol, 22, pp.1981~1989 (2011)
- Gennari FJ, "Role of the kidney in potassium homeostasis : lessons from acid-base disturbances", Kidney Int, 8, pp.1~5 (1975)
- Roy A, "Collecting duct intercalated cell function and regulation", Clin J Am Soc Nephrol, 10, pp.305~324 (2015)
- Kim J, "Intercalated cell subtypes in connecting tubule and cortical collecting duct of rat and mouse", J Am Soc Nephrol, 10, pp.1~12 (1999)
- Han KH, "Expression of ammonia transporter family members, Rh B glycoprotein and Rh C glycoprotein, in the developing rat kidney", Am J Physiol Renal Physiol, 299, pp.F187~F198 (2010)
- Han KH, "Effect of hypokalemia on renal expression of the ammonia transporter family members, Rh B Glycoprotein and Rh C Glycoprotein, in the rat kidney", Am J Physiol Renal Physiol, 301, pp.F823~F832 (2011)
- Kim HY, "Effect of reduced renal mass on renal ammonia transporter family, Rh C glycoprotein and Rh B glycoprotein, expression", Am J Physiol Renal Physiol, 293, pp.F1238~F1247 (2007)
- Park EY, "Proposed mechanism in the change of cellular composition in the outer medullary collecting duct during potassium homeostasis", Histol Histopathol, 27, pp.1559~1577 (2012)
- Kim YH, "Immunocytochemical localization of pendrin in intercalated cell subtypes in rat and mouse kidney", Am J Physiol Renal Physiol, 283, pp.F744~F754 (2002)
- Wall SM, "Localization of pendrin in mouse kidney", Am J Physiol Renal Physiol, 284, pp.F229~F241 (2003)
- Fung TT, "Dietary patterns and the risk of coronary heart disease in women", Arch Intern Med, 161, pp.1857~1862 (2001)
- Cohn JN, "New guidelines for potassium replacement in clinical practice : a contemporary review by the National Council on Potassium in Clinical Practice", Arch Intern Med, 160, pp.2429~2436 (2000)
- Wall SM, "The role of pendrin in blood pressure regulation", Am J Physiol Renal Physiol, 310, pp.F193~F203 (2016)
- Jacques T, "Overexpression of pendrin in intercalated cells produces chloride-sensitive hypertension", J Am Soc Nephrol, 24, pp.1104~1113 (2013)
- Susan M. Wall, "Renal intercalated cells and blood pressure regulation", Kidney Research and Clinical Practice, 36(4), pp.305~317 (2017), 대한신장학회
- Wall SM, "The Renal Physiology of Pendrin-Positive Intercalated Cells", Physiol Rev, 100, pp.1119~1147 (2020)
- Verlander JW, "Localization of the ammonium transporter proteins RhBG and RhCG in mouse kidney", Am J Physiol Renal Physiol, 284, pp.F323~F337 (2003)