Capsaicin, a component of red peppers, stimulates protein kinase CKII activity
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Protein kinase CKII (CKII), a heterotetramer composed of two catalytic (α or α’) subunits and two regulatory (β) subunits,plays a critical role in cell proliferation and anti-apoptosis. Recently,capsaicin was shown to trigger apoptosis. Therefore,we examined the effect of capsaicin on CKII activity. Although capsaicin induced apoptotic death in HeLa cells, CKII activity was increased in the cytosolic fraction of HeLa cells after treatment. Capsaicin did not change the expression of the CKIIαand CKIIβ proteins. Capsaicin stimulated the catalytic activity of recombinant CKII tetramer, but not the CKIIα subunit. Moreover,capsaicin enhanced the autophosphorylation of CKIIαand CKIIβ. Taken together, our data suggest that capsaicin stimulates the phosphotransferase activity of CKII holoenzyme by interacting with the CKIIβ subunit.
Abstract
Protein kinase CKII (CKII), a heterotetramer composed of two catalytic (α or α’) subunits and two regulatory (β) subunits,plays a critical role in cell proliferation and anti-apoptosis. Recently,capsaicin was shown to trigger apoptosis. Therefore,we examined the effect of capsaicin on CKII activity. Although capsaicin induced apoptotic death in HeLa cells, CKII activity was increased in the cytosolic fraction of HeLa cells after treatment. Capsaicin did not change the expression of the CKIIαand CKIIβ proteins. Capsaicin stimulated the catalytic activity of recombinant CKII tetramer, but not the CKIIα subunit. Moreover,capsaicin enhanced the autophosphorylation of CKIIαand CKIIβ. Taken together, our data suggest that capsaicin stimulates the phosphotransferase activity of CKII holoenzyme by interacting with the CKIIβ subunit.
참고문헌 (23)
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