Tetrahydropteridines possess antioxidant roles to guard against glucose-induced oxidative stress in Dictyostelium discoideum
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Glucose effects on the vegetative growth of Dictyostelium discoideum Ax2 were studied by examining oxidative stress and tetrahydropteridine synthesis in cells cultured with different concentrations (0.5X, 7.7 g L-1; 1X, 15.4 g L-1; 2X, 30.8 g L-1) of glucose. The growth rate was optimal in 1X cells (cells grown in 1X glucose) but was impaired drastically in 2X cells, below the level of 0.5X cells. There were glucose-dependent increases in reactive oxygen species (ROS) levels and mitochondrial dysfunction in parallel with the mRNA copy numbers of the enzymes catalyzing tetrahydropteridine synthesis and regeneration. On the other hand, both the specific activities of the enzymes and tetrahydropteridine levels in 2X cells were lower than those in 1X cells, but were higher than those in 0.5X cells. Given the antioxidant function of tetrahydropteridines and both the beneficial and harmful effects of ROS, the results suggest glucose-induced oxidative stress in Dictyostelium, a process that might originate from aerobic glycolysis, as well as a protective role of tetrahydropteridines against this stress.
Abstract
Glucose effects on the vegetative growth of Dictyostelium discoideum Ax2 were studied by examining oxidative stress and tetrahydropteridine synthesis in cells cultured with different concentrations (0.5X, 7.7 g L-1; 1X, 15.4 g L-1; 2X, 30.8 g L-1) of glucose. The growth rate was optimal in 1X cells (cells grown in 1X glucose) but was impaired drastically in 2X cells, below the level of 0.5X cells. There were glucose-dependent increases in reactive oxygen species (ROS) levels and mitochondrial dysfunction in parallel with the mRNA copy numbers of the enzymes catalyzing tetrahydropteridine synthesis and regeneration. On the other hand, both the specific activities of the enzymes and tetrahydropteridine levels in 2X cells were lower than those in 1X cells, but were higher than those in 0.5X cells. Given the antioxidant function of tetrahydropteridines and both the beneficial and harmful effects of ROS, the results suggest glucose-induced oxidative stress in Dictyostelium, a process that might originate from aerobic glycolysis, as well as a protective role of tetrahydropteridines against this stress.
참고문헌 (25)
- Klein, R., "Dictyopterin, 6-(D-threo-1,2-dihydroxypropyl)-pterin, a new natural isomer of L-biopterin. Isolation from vegetative cells of Dictyostelium discoideum and identification", Eur. J. Biochem, 187, pp.665~669 (1990)
- Werner, E. R., "Tetrahydrobiopterin: biochemistry and pathophysiology", Biochem. J, 438, pp.397~414 (2011)
- Hye-Lim Kim, "Maintenance of cellular tetrahydrobiopterin homeostasis", BMB Reports, 43(9), pp.584~592 (2010), 생화학분자생물학회
- Kim, H. L, "Tetrahydropteridine deficiency impairs mitochondrial function in Dictyostelium discoideum Ax2", FEBS Lett, 581, pp.5430~5434 (2007)
- Kim, H. L., "Tetrahydrobiopterin is functionally distinguishable from tetrahydrodictyopterin in Dictyostelium discoideum Ax2", FEBS Lett, 585, pp.3047~3051 (2011)
- Choi, Y. K., "D-threo-tetrahydrobiopterin is synthesized via 1’-oxo-2'-D-hydroxy-tetrahydropterin in Dictyostelium discoideum Ax2", FEBS Lett, 579, pp.3085~3089 (2005)
- Franke, J., "A defined minimal medium for axenic strains of D. Discoideum", Proc. Natl. Acad. Sci. U.S.A, 74, pp.2157~2161 (1977)
- Lu, Y. H., "Influence of medium components on growth kinetics of Dictyostelium discoideum", World J. Microbiol. Biotechnol, 24, pp.491~499 (2008)
- Brownlee, M., "Biochemistry and molecular cell biology of diabetic complications", Nature, 414, pp.813~820 (2001)
- Landolfo, S., "ROS accumulation and oxidative damage to cell structures in Saccharomyces cerevisiae wine strains during fermentation of high-sugar-containing medium", Biochim. Biophys. Acta, 1780, pp.892~898 (2008)
- Cotter, D. A., "Germination and mitochondrial damage in spores of Dictyostelium discoideum following supraoptimal heating", Arch. Microbiol, 103, pp.163~168 (1975)
- Bacon, C. W, "Effects of the Self-Inhibitor of Dictyostelium discoideum on Spore Metabolism", J. Gen. Microbiol, 76, pp.331~344 (1973)
- Bartosz, G., "Reactive oxygen species: Destroyers or messengers?", Biochem. Pharmacol, 77, pp.1303~1315 (2009)
- Trachootham, D., "Targeting cancer cells by ROS-mediated mechanisms: a radical therapeutic approach?", Nature Reviews Drug Discovery, 8, pp.579~591 (2009)
- Davis, K. J., "Degradation of oxidized proteins by the 20S proteasome", Biochimie, 83, pp.301~310 (2001)
- Schauer, T. M., "Proteasomes from Dictyostelium discoideum: characterization of structure and function", J. Struct. Biol, 111, pp.135~147 (1993)
- Pfeiffer, T., "Cooperation and competition in the evolution of ATP-producing pathways", Science, 292, pp.504~507 (2001)
- Lunt, S. Y., "Aerobic glycolysis: meeting the metabolic requirements of cell proliferation", Annu. Rev. Cell Dev. Biol, 27, pp.441~464 (2011)
- Barros, M. H., "Higher respiratory activity decreases mitochondrial reactive oxygen release and increases life span in Saccharomyces cerevisiae", J. Biol. Chem, 279, pp.49883~49888 (2004)
- Brand, K. A., "Aerobic glycolysis by proliferating cells: a protective strategy against reactive oxygen species", FASEB J, 11, pp.388~395 (1997)
- Turrens, J. F., "Mitochondrial formation of reactive oxygen species", J. Physiol, 552, pp.335~344 (2003)
- Watts, D. J., "Growth of myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture", Biochem. J, 119, pp.171~174 (1970)
- Morrissey, J. H., "Pattern formation in Dictyostelium discoideum: an analysis of mutants altered in cell proportioning", Dev. Biol, 15, pp.1~8 (1981)
- Choi, Y. K., "Functional role of sepiapterin reductase in the biosynthesis of tetrahydropteridines in Dictyostelium discoideum Ax2", Biochim. Biophys. Acta, 1760, pp.877~882 (2006)
- Xu, J., "Proteasome-dependent degradation of guanosine 5’-triphosphate cyclohydrolase I causes tetrahydrobiopterin deficiency in diabetes mellitus", Circulation, 116, pp.944~953 (2007)