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Human selenium binding protein-1 (hSP56) is a negative regulator of HIF-1α and suppresses the malignant characteristics of prostate cancer cells

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In the present study, we demonstrate that ectopic expression of56-kDa human selenium binding protein-1 (hSP56) in PC-3cells that do not normally express hSP56 results in a markedinhibition of cell growth in vitro and in vivo. Down-regulationof hSP56 in LNCaP cells that normally express hSP56 results inenhanced anchorage-independent growth. PC-3 cellsexpressing hSP56 exhibit a significant reduction of hypoxiainducible protein (HIF)-1α protein levels under hypoxicconditions without altering HIF-1α mRNA (HIF1A) levels. Taken together, our findings strongly suggest that hSP56 plays acritical role in prostate cells by mechanisms including negativeregulation of HIF-1α, thus identifying hSP56 as a candidateanti-oncogene product.

Abstract

In the present study, we demonstrate that ectopic expression of56-kDa human selenium binding protein-1 (hSP56) in PC-3cells that do not normally express hSP56 results in a markedinhibition of cell growth in vitro and in vivo. Down-regulationof hSP56 in LNCaP cells that normally express hSP56 results inenhanced anchorage-independent growth. PC-3 cellsexpressing hSP56 exhibit a significant reduction of hypoxiainducible protein (HIF)-1α protein levels under hypoxicconditions without altering HIF-1α mRNA (HIF1A) levels. Taken together, our findings strongly suggest that hSP56 plays acritical role in prostate cells by mechanisms including negativeregulation of HIF-1α, thus identifying hSP56 as a candidateanti-oncogene product.

참고문헌 (31)

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