Tight junction protein 1 is regulated by transforming growth factor-β and contributes to cell motility in NSCLC cells
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Tight junction protein 1 (TJP1), a component of tight junction,has been reported to play a role in protein networks as anadaptor protein, and TJP1 expression is altered during tumordevelopment. Here, we found that TJP1 expression was increasedat the RNA and protein levels in TGF-β-stimulatedlung cancer cells, A549. SB431542, a type-I TGF-β receptorinhibitor, as well as SB203580, a p38 kinase inhibitor, significantlyabrogated the effect of TGF-β on TJP1 expression. Diphenyleneiodonium, an NADPH oxidase inhibitor, also attenuatedTJP1 expression in response to TGF-β in lung cancercells. When TJP1 expression was reduced by shRNA lentiviralparticles in A549 cells (A549-sh TJP1), wound healing wasmuch lower than in cells infected with control viral particles. Taken together, these data suggest that TGF-β enhances TJP1expression, which may play a role beyond structural supportin tight junctions during cancer development.
Abstract
Tight junction protein 1 (TJP1), a component of tight junction,has been reported to play a role in protein networks as anadaptor protein, and TJP1 expression is altered during tumordevelopment. Here, we found that TJP1 expression was increasedat the RNA and protein levels in TGF-β-stimulatedlung cancer cells, A549. SB431542, a type-I TGF-β receptorinhibitor, as well as SB203580, a p38 kinase inhibitor, significantlyabrogated the effect of TGF-β on TJP1 expression. Diphenyleneiodonium, an NADPH oxidase inhibitor, also attenuatedTJP1 expression in response to TGF-β in lung cancercells. When TJP1 expression was reduced by shRNA lentiviralparticles in A549 cells (A549-sh TJP1), wound healing wasmuch lower than in cells infected with control viral particles. Taken together, these data suggest that TGF-β enhances TJP1expression, which may play a role beyond structural supportin tight junctions during cancer development.
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