HCBP6 upregulates human SREBP1c expression by binding to C/EBPβ-binding site in the SREBP1c promoter
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초록
Sterol regulatory element-binding protein-1c (SREBP1c) plays an important role in triglyceride (TG) homeostasis. Although our previous study showed that hepatitis C virus core-binding protein 6 (HCBP6) regulates SREBP1c expression to maintain intracellular TG homeostasis, the mechanism underlying this regulation is unclear. In the present study, we found that HCBP6 increased intracellular TG levels by upregulating SREBP1c expression. HCBP6 increased SREBP1c transcription by directly binding to the SREBP1c promoter (at the -139- to +359-bp region). Moreover, we observed that HCBP6 interacted with C/EBPβ-binding site in the SREBP1c promoter both in vitro and in vivo. These results indicate that HCBP6 upregulates human SREBP1c expression by binding to the C/EBPβ-binding site in the SREBP1c promoter.
Abstract
Sterol regulatory element-binding protein-1c (SREBP1c) plays an important role in triglyceride (TG) homeostasis. Although our previous study showed that hepatitis C virus core-binding protein 6 (HCBP6) regulates SREBP1c expression to maintain intracellular TG homeostasis, the mechanism underlying this regulation is unclear. In the present study, we found that HCBP6 increased intracellular TG levels by upregulating SREBP1c expression. HCBP6 increased SREBP1c transcription by directly binding to the SREBP1c promoter (at the -139- to +359-bp region). Moreover, we observed that HCBP6 interacted with C/EBPβ-binding site in the SREBP1c promoter both in vitro and in vivo. These results indicate that HCBP6 upregulates human SREBP1c expression by binding to the C/EBPβ-binding site in the SREBP1c promoter.
참고문헌 (34)
- Tilg H, "NAFLD and diabetes mellitus", Nat Rev Gastroenterol Hepatol, 1, pp.32~42 (2017)
- Jin J, "Increased expression of enzymes of triglyceride synthesis is essential for the development of hepatic steatosis", Cell Rep, 3, pp.831~843 (2013)[DOI]
- Farrell GC, "NAFLD in Asia--as common and important as in the West", Nat Rev Gastroenterol Hepatol, 5, pp.307~318 (2013)
- Hua X, "Hairpin orientation of sterol regulatory element-binding protein-2 in cell membranes as determined by protease protection", J Biol Chem, 49, pp.29422~29427 (1995)
- Lee SJ, "The structure of importin-beta bound to SREBP-2: nuclear import of a transcription factor", Science (New York, NY), 5650, pp.1571~1575 (2003)
- Eberle D, "SREBP transcription factors: master regulators of lipid homeostasis", Biochimie, 11, pp.839~848 (2004)
- Zhang X, "Liver X receptor activation increases hepatic fatty acid desaturation by the induction of SCD1 expression through an LXRalpha-SREBP1c-dependent mechanism", J Diabetes, 3, pp.212~220 (2014)
- Liu L, "Arginine Methylation of SREBP1a via PRMT5 Promotes De Novo Lipogenesis and Tumor Growth", Cancer Res, 5, pp.1260~1272 (2016)
- Park SH, "Epithelial Cholesterol Deficiency Attenuates Human Antigen R-linked Pro-inflammatory Stimulation via an SREBP2-linked Circuit", J Biol Chem, 47, pp.24641~24656 (2016)
- Deng X, "Regulation of the rat SREBP-1c promoter in primary rat hepatocytes", Biochem Biophys Res Commun, 1, pp.256~262 (2002)
- Cagen LM, "Insulin activates the rat sterol-regulatoryelement-binding protein 1c (SREBP-1c) promoter through the combinatorial actions of SREBP, LXR, Sp-1 and NF-Y cis-acting elements", Biochem J, pp.207~216 (2005)
- Payne Victoria A, "C", EBP transcription factors regulateSREBP1cgene expression during adipogenesis, 2010, pp.1, 215~224
- "Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi", 2002, 351~353
- Gao LL, "HCBP6 Modulates Triglyceride Homeostasis in Hepatocytes Via the SREBP1c", FASN Pathway, 2015, pp.10, 2375~2384
- Li K, "Hepatitis C virus protein binding protein", World Chinese J Digestol, 2, pp.215~217 (2002)
- Li Q, "Hepatitis C virus infection affects lipid metabolism", World Chinese J Digestol, 12, pp.1951~1954 (2003)
- Cheng J, "Correlation between hepatitis C virus infection and lipid metabolism", Chinese Hepatol, 1, pp.56~58 (2002)
- Li M, "MicroRNA-185-5p mediates regulation of SREBP2 expression by hepatitis C virus core protein", World J Gastroenterol, 15, pp.4517~4525 (2015)
- Khan M, "Interaction of the hepatitis C virus (HCV) core with cellular genes in the development of HCV-induced steatosis", Arch Virol, 11, pp.1735~1753 (2010)
- Kim KH, "HCV core protein induces hepatic lipid accumulation by activating SREBP1 and PPARgamma", Biochem Biophys Res Commun, 4, pp.883~888 (2007)
- Yamaguchi A, "Hepatitis C virus core protein modulates fatty acid metabolism and thereby causes lipid accumulation in the liver", Dig Dis Sci, 7, pp.1361~1371 (2005)
- Wang L, "Identification and evaluation promoter sequence and the transcription activation of Hcbp6 interaction with core protein of hepatitis C virus", Chinese J Hepatol, 2, pp.81~85 (2006)
- Jang H, "SREBP1c-CRY1 signalling represses hepatic glucose production by promoting FOXO1 degradation during refeeding", Nature Commun, pp.12180~ (2016)
- Wang Y, "Transcriptional regulation of hepatic lipogenesis", Nature Rev Mol Cell Biol, 11, pp.678~689 (2015)
- Deng X, "Expression of the rat sterol regulatory element-binding protein-1c gene in response to insulin is mediated by increased transactivating capacity of specificity protein 1 (Sp1)", J Biol Chem, 24, pp.17517~17529 (2007)
- Dif N, "Insulin activates human sterol-regulatory-elementbinding protein-1c (SREBP-1c) promoter through SRE motifs", Biochem J, 1, pp.179~188 (2006)
- Shen L, "Hepatic differentiated embryo-chondrocyte-expressed gene 1 (Dec1) inhibits sterol regulatory element-binding protein-1c (Srebp-1c) expression and alleviates fatty liver phenotype", J Biol Chem, 34, pp.23332~23342 (2014)
- Cheng J, "Molecular biological mechanism of hepatitis virus interaction with hepatocytes", Zhong Xi Yi Jie He Gan Bing Za Zhi, 6, pp.3~ (2003)
- Cheng J, "Study on cloning of new gene related to pathogenesis of chronic hepatitis virus infection", Da Lian Da Xue Xue Bao, 4, pp.4~ (2006)
- Tian J, "Insulin induction of SREBP-1c in rodent liver requires LXRalpha-C", EBPbeta complex, 2016, pp.29, 8182~8187
- Schroeder-Gloeckler JM, "CCAAT", enhancer-binding protein beta deletion reduces adiposity, hepatic steatosis, and diabetes in Lepr(db, J Biol Chem, pp.2007, 21[DOI]
- Rahman SM, "CCAAT", enhancing binding protein beta deletion in mice attenuates inflammation, endoplasmic reticulum stress, and lipid accumulation in diet-induced nonalcoholic steatohepatitis, 2007, pp.5, 1108~1117
- Xi Y, "HMGA2 promotes adipogenesis by activating C", EBPbeta-mediated expression of PPARgamma, 2016, pp.4, 617~623
- Chen G, "Central role for liver X receptor in insulin-mediated activation of Srebp-1c transcription and stimulation of fatty acid synthesis in liver", Proc Natl Acad Sci U S A, 31, pp.11245~11250 (2004)
