본문 바로가기
KERD
논문KCI 등재

PEP-1-GLRX1 Protein Exhibits Anti-Inflammatory Effects by Inhibiting the Activation of MAPK and NF-κB Pathways in Raw 264.7 Cells

2

조회

0

다운로드

1

인용

0

참고문헌

키워드

주제분류화학

초록

Glutaredoxin 1 (GLRX1) has been recognized as an important regulator of redox signaling. Although GLRX1 plays an essential role in cell survival as an antioxidant protein, the function of GLRX1 protein in inflammatory response is still under investigation. Therefore, we wanted to know whether transduced PEP-1-GLRX1 protein inhibits lipopolysaccharide (LPS)- and 12-O-tetradecanoyl phorbol-13-acetate (TPA)-induced inflammation. In LPS-exposed Raw 264.7 cells, PEP-1-GLRX1 inhibited cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), activation of mitogen activated protein kinases (MAPKs) and nuclear factor-kappaB (NF-κB) expression levels. In a TPA-induced mouse-ear edema model, topically applied PEP-1-GLRX1 transduced into ear tissues and significantly ameliorated ear edema. Our data reveal that PEP-1-GLRX1 attenuates inflammation in vitro and in vivo, suggesting that PEP-1-GLRX1 may be a potential therapeutic protein for inflammatory diseases.

Abstract

Glutaredoxin 1 (GLRX1) has been recognized as an important regulator of redox signaling. Although GLRX1 plays an essential role in cell survival as an antioxidant protein, the function of GLRX1 protein in inflammatory response is still under investigation. Therefore, we wanted to know whether transduced PEP-1-GLRX1 protein inhibits lipopolysaccharide (LPS)- and 12-O-tetradecanoyl phorbol-13-acetate (TPA)-induced inflammation. In LPS-exposed Raw 264.7 cells, PEP-1-GLRX1 inhibited cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), activation of mitogen activated protein kinases (MAPKs) and nuclear factor-kappaB (NF-κB) expression levels. In a TPA-induced mouse-ear edema model, topically applied PEP-1-GLRX1 transduced into ear tissues and significantly ameliorated ear edema. Our data reveal that PEP-1-GLRX1 attenuates inflammation in vitro and in vivo, suggesting that PEP-1-GLRX1 may be a potential therapeutic protein for inflammatory diseases.

참고문헌 (54)

  1. Medzhitov R, "Origin and physiological roles of inflammation", Nature, 454, pp.428~435 (2008)
  2. Tsaryk R, "The role of oxidative stress in pro-inflammatory activation of human endothelial cells on Ti6A14V alloy", Biomaterials, 34, pp.8075~8085 (2013)
  3. Fujiwara N, "Macrophages in inflammation", Curr Drug Targets Inflamm Allergy, 4, pp.281~286 (2005)
  4. Hiraiwa K, "Contribution of lung macrophages to the inflammatory responses induced by exposure to air pollutants", Mediators Inflamm, 2013, pp.619523~ (2013)
  5. Jin SE, "Anti-inflammatory effect and action mechanisms of traditional herbal formula Gamisoyo-san in RAW 264.7 macrophages", BMC Complement Altern Med, 16, pp.219~ (2016)
  6. Shen YZ, "Citral inhibits lipopolysaccharide-induced acute lung injury by activating PPAR-gamma", Eur J Pharmacol, 747, pp.45~51 (2015)
  7. Guo C, "Sophoraflavanone G from Sophora alopecuroides inhibits lipopolysaccharide-induced RAW 264.7 cells by targeting PI3K", Akt, JAK, HO-1 pathways, pp.Int Immunopharmacol, 2016[DOI]
  8. Ducut Sigala JL, "Activation of transcription factor NF-kappaB requires ELKS, an IkappaB kinase regulatory subunit", Science, 304, pp.1963~1967 (2004)
  9. Wang Y, "Gangliosdie GD 1a suppresses LPS-induced pro-inflammatory cytokines in RAW 264.7 macrophages by reducing MAPKs and NF-kappaB signaling pathways through TLR4", Int Immunopharmacol, 28, pp.136~145 (2015)
  10. Li MY, "Astaxanthin protects lipopolysaccharide-induced inflammatory response in Channa argus through inhibiting NF-B and MAPKs signaling pathways", Fish Shellfish Immunol, 86, pp.280~286 (2018)
  11. Haque MA, "Zerumbone suppresses the activation of inflammatory mediators in LPS-stimulated U937 macrophages through MyD88-dependent NF-B", MAPK, Int Immunopharmacol, pp.2018, 55[DOI]
  12. Arthur JS, "Mitogen-activated protein kinases in innate immunity", Nat Rev Immunol, 13, pp.679~692 (2013)
  13. Hwang PA, "Inhibition of lipopolysaccharide (LPS)-induced inflammatory responses by Sargassum hemiphyllum sulfated polysaccharide extract in RAW 264.7 macrophage cells", J Agric Food Chem, 59, pp.2062~2068 (2011)
  14. Lillig CH, "Glutaredoxin systems", Biochim Biophys Acta, 1780, pp.1304~1317 (2008)
  15. Okuda M, "Expression of glutaredoxin in human coronary arteries: its potential role in antioxidant protection against atherosclerosis", Arterioscler Thromb Vasc Biol, 21, pp.1483~1487 (2001)
  16. Pai HV, "What is the functional significance of the unique location of glutaredoxin 1 (GRx1) in the intermembrane space of mitochondria?", Antioxid Redox Signal, 9, pp.2027~2033 (2007)
  17. Peltoniemi M, "Expression of glutaredoxin is highly cell specific in human lung and is decreased by transforming growth factor-beta in vitro and in interstitial lung diseases in vivo", Hum Pathol, 35, pp.1000~1007 (2004)
  18. Cater MA, "Glutaredoxin1protects neuronal cells from copper-induced toxicity", Biometals, 27, pp.661~672 (2014)
  19. Ryu EJ, "PEP-1-glutaredoxin 1protects against hippocampal neuronal cell damage from oxidative stress via regulation of MAPK and apoptotic signaling pathways", Mol Med Rep, 18, pp.2216~2228 (2018)
  20. Joliot A, "Transduction peptides : from technology to physiology", Nat Cell Biol, 6, pp.189~196 (2004)
  21. Dolgova NV, "Copper chaperone Atox1 interacts with the metal-binding domain of Wilson's disease protein in cisplatin detoxification", Biochem J, 454, pp.147~156 (2013)
  22. Wadia JS, "Protein transduction technology", Curr Opin Biotechnol, 13, pp.52~56 (2002)
  23. van den Berg A, "Protein transduction domain delivery of therapeutic macromolecules", Curr Opin Biotechnol, 22, pp.888~893 (2011)
  24. Morris MC, "A peptide carrier for the delivery of biologically active proteins into mammalian cells", Nat Biotechonol, 19, pp.1173~1176 (2001)
  25. 여현지, "Protective effects of Tat-DJ-1 protein against streptozotocin-induced diabetes in a mice model", BMB Reports, 51(7), pp.362~367 (2018), 생화학분자생물학회
  26. Yeo HJ, "Tat-CIAPIN1 inhibits hippocampal neuronal cell damage through the MAPK and apoptotic signaling pathways", Free Radic Biol Med, 135, pp.68~78 (2019)
  27. 김대원, "Tat-ATOX1 inhibits inflammatory responses via regulation of MAPK and NF-κB pathways", BMB Reports, 51(12), pp.654~659 (2018), 생화학분자생물학회
  28. Kim MJ, "Transduced PEP-1-PON1 proteins regulate microglial activation and dopaminergic neuronal death in a Parkinson's disease model", Biomaterials, 64, pp.45~56 (2015)
  29. Kim YN, "Neuroprotective effects of PEP-1-carbonyl reductase 1 against oxidativestress-induced ischemic neuronal cell damage", Free Radic Biol Med, 69, pp.181~196 (2014)
  30. 이수진, "PEP-1-paraoxonase 1 fusion protein prevents cytokine-induced cell destruction and impaired insulin secretion in rat insulinoma cells", BMB Reports, 51(10), pp.538~543 (2018), 생화학분자생물학회
  31. Chung S, "Glutaredoxin 1 regulates cigarette smoke-mediated lung inflammation through differential modulation of IB kinases in mice : impact on histone acetylation", Am J Physiol Lung Cell Mol Physiiol, 299, pp.L192~L203 (2010)
  32. Malik G, "Role of glutaredoxin-1 in cardioprotection : an insight with Glrx1transgenic and knockout animals", J Mol Cell Cardiol, 44, pp.261~269 (2008)
  33. Meyer LM, "Absence of glutaredoxn1 increase lens susceptibility to oxidative stress induced by UVR-B", Exp Eye Res, 89, pp.833~839 (2009)
  34. Aesif SW, "Ablation of glutaredoxin-1 attenuates lipopolysaccharide-induced lung inflammation and alveolar macrophage activation", Am J Respir Cell Mol Biol, 44, pp.491~499 (2011)
  35. Shi Q, "Geniposde suppresses LPS-induced nitric oxide, PGE2 and inflammatory cytokine by downregulating NF-kappaB, MAPK and AP-1 signaling pathways in macrophages", Int Immunopharmacol, 20, pp.298~306 (2014)
  36. Chen H, "Anti-inflammatory effects of chicanine on murine macrophage by down-regulating LPS-induced inflammatory cytokines in IkappaBalpha", MAPK, Eur J Pharmacol, pp.2014, 724[DOI]
  37. Yodkeeree S, "O-methybulbocapnine and dicentrine suppress LPS-induced inflammatory responses by blocking NF-B and AP-1 inactivation through inhibition MAPKs and Akt signaling in RAW264.7 macrophages", Biol Pharm Bull, 41, pp.1219~1227 (2018)
  38. Plotnikov A, "The MAPK cascades : signaling components, nuclear roles and mechanisms of nuclear translocation", Biochim Biophys Acta, 1813, pp.1619~1633 (2011)
  39. Qiang Z, "Inhibitory effect of different Dendrobium species on LPS-induced inflammation in macrophages via suppression of MAPK pathways", Chin J Nat Med, 16, pp.481~489 (2018)
  40. Harikrishnan H, "Anti-inflammatory effect of phyllanthus amarus Schum. &Thonn. Through inhibition of NF-κB, MAPK, and PI3K-Akt signaling pathways in LPS-induced human macrophages", BMC Complement Altern Med, 18, pp.224~ (2018)
  41. Islam SU, "Decursinol angelate inhibits LPS-induced macrophages polarization through modulation of the NF-κB and MAPK signaling pathways", Molecules, 23, pp.1880~ (2018)
  42. Reynaert NL, "Dynamic redox control of NF-kappaB through glutaredoxin-regulated S-glutathionylation of inhibitory kappaB kinase beta", Proc Natl Acad Sci U S A, 103, pp.13086~13091 (2006)
  43. Reynaert NL, "Modulation of glutaredoxin-1 expression in a mouse model of allergic airway disease", Am J Respir Cell Mol Biol, 36, pp.147~151 (2007)
  44. Poynter ME, "Rapid activation of nuclear factor-kappaB in airway epithelium in a murine model of allergic airway inflammation", Am J Pathol, 160, pp.1325~1334 (2002)
  45. Shelton MD, "Glutaredoxin regulates autocrine and paracrine proinflammatory responses in retinal glial(Muller)cells", J Biol Chem, 284, pp.4760~4766 (2009)
  46. El-Andaloussi S, "Cell-penetrating peptides : mechanisms and applications", Curr Pharm Des, 11, pp.3597~3611 (2005)
  47. Stanley PL, "Mouse skin inflammation induced by multiple topical applications of 12-O-tetradecanoyl phorbol-13-acetate", Skin Pharmacol, 4, pp.262~271 (1991)
  48. Kim MJ, "PEP-1-PON1 protein regulates inflammatory response in raw 264.7 macrophages and ameliorates inflammation in a TPA-induced animal model", PLoS One, 9, pp.e86034~ (2014)
  49. 박미경, "Suppression of Transglutaminase-2 is Involved in Anti-Inflammatory Actions of Glucosamine in 12-OTetradecanoylphorbol- 13-Acetate-Induced Skin Inflammation", Biomolecules & Therapeutics, 20(4), pp.380~385 (2012), 한국응용약물학회[DOI]
  50. Nagaraj M. Kulkarni, "Topical atorvastatin ameliorates 12-O-tetradecanoylphorbol-13- acetate induced skin inflammation by reducing cutaneous cytokine levels and NF-jB activation", Archives of Pharmacal Research, 38(6), pp.1238~1247 (2015), 대한약학회
  51. Kamiya T, "Copper chaperone antixodiant-1, Atox-1, is involved in the induction of SOD3 in THP-1 cells", Biometals, 31, pp.61~68 (2018)
  52. Kim SH, "Overexpression of extracellular superoxide dismutase(EC-SOD)in mouse skin plays a protective role in DMBA", TPA-induced tumor formation, 2005, pp.15, 333~341
  53. Bradford M, "A rapid and sensitive method for the quantitation of microgram quantities utilizing the principle of protein-dye binding", Anal Biochem, 72, pp.248~254 (1976)
  54. Ahn EH, "PEP-1-PEA-15 protects against toxin-induced neuronal damage in a mouse model of Parkinson’s disease", Biochim Biophys Acta, 1840, pp.1686~1700 (2014)

관련 논문AI추천

Tat-ATOX1 inhibits inflammatory responses via regulation of MAPK and NF-κB pathways권현정, 김대원, 박진서, 신민재, 음원식, 이성호, 이승호, 최수영, 최연주, 한규형생화학분자생물학회 · BMB Reports2018 · 제51권 제12호Tat-GSTpi suppresses inflammatory responses by regulating ROS/MAPKs/apoptosis signaling pathwaysChan Hee Lee, Dae Won Kim, Duk-Soo Kim, Eun Ji Yeo, Gi Soo Youn, Hyeon Ji Yeo, Hyun Jung Kwon, Jong Kook Park, Jung Hwan Park, Keun Wook Lee, Lee Re Lee, Min Jea Shin, Na Yeon Kim, Soo Young Choi, Su Min Kim, Su Yeon Kwon, Won Sik Eum, Yeon Joo Choi생화학분자생물학회 · BMB Reports2025 · 제58권 제6호Transduced Tat-Annexin protein suppresses inflammationassociated gene expression in lipopolysaccharide (LPS)-stimulated Raw 264.7 cells강태천, 권오신, 김대원, 박은영, 박종훈, 박진서, 백수선, 음원식, 이선화, 조성우, 최수영, 한규형, 황현숙생화학분자생물학회 · BMB Reports2011 · 제44권 제7호Anti-inflammatory effects of proanthocyanidin-rich red rice extract via suppression of MAPK, AP-1 and NF-κB pathways in Raw 264.7 macrophagesPornngarm Limtrakul, Pornsiri Pitchakarn, Supachai Yodkeeree, Wanisa Punfa한국영양학회 · Nutrition Research and Practice2016 · 제10권 제3호Transduced Tat-CIAPIN1 reduces the inflammatory response on LPS- and TPA-induced damages김대원, 김덕수, 김민영, 김정수, 신민재, 엄원식, 여현지, 유지호, 최수영생화학분자생물학회 · BMB Reports2019 · 제52권 제12호Anti-inflammatory effects of a novel compound, MPQP, through the inhibition of IRAK1 signaling pathways in LPS-stimulated RAW 264.7 macrophages김바름, 조사연, 조영창생화학분자생물학회 · BMB Reports2018 · 제51권 제6호

이 간행물의 다른 논문 (8건)